FlyBase Allele Report: Dmel\Rab5[HMS00147]

General Information

Symbol

Dmel\Rab5^HMS00147

Species

D. melanogaster

Name

FlyBase ID

FBal0263144

Feature type

allele

Associated gene

Dmel\Rab5

Associated Insertion(s)

Carried in Construct

P{TRiP.HMS00147}

Also Known As

Rab5 RNAi, Rab5^RNAi, UAS-Rab5-RNAi, HMS00147, UAS-Rab5 RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2011.7.19)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2011.7.19)

Mutagen

in vitro construct

(Ni et al., 2011.7.19)

Progenitor genotype

Carried in construct

P{TRiP.HMS00147}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2011.7.19)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMS00147

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of kidney disease

CEA

(Xi et al., 2024)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates amyotrophic lateral sclerosis type 15

modeled by Hsap\UBQLN2^WT.UAS

(Kim et al., 2023)

modeled by Hsap\UBQLN2^P4X.UAS

(Kim et al., 2023)

modeled by Hsap\UBQLN2^P497H.UAS

(Kim et al., 2023)

exacerbates amyotrophic lateral sclerosis type 8

modeled by Vap33^{P58S.UAS.Tag:FLAG}

(Sanhueza et al., 2015)

ameliorates epilepsy

modeled by zyd¹

(Weiss et al., 2019)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | third instar larval stage, with Hsim\VP16^AD.nrv2, Scer\GAL4^{DBD.wrapper.932i}

(Coutinho-Budd et al., 2017)

melanotic mass phenotype, with Scer\GAL4^{SPARC-MI00329-GAL4}

(Zang et al., 2015)

Phenotype Manifest In

embryonic/larval cell body glial cell | third instar larval stage, with Hsim\VP16^AD.nrv2, Scer\GAL4^{DBD.wrapper.932i}

(Coutinho-Budd et al., 2017)

fat body, with Scer\GAL4^{SPARC-MI00329-GAL4}

(Zang et al., 2015)

melanotic mass, with Scer\GAL4^{SPARC-MI00329-GAL4}

(Zang et al., 2015)

nephrocyte | larval stage, with Scer\GAL4^sns.PU

(Xi et al., 2024)

nephrocyte diaphragm | larval stage, with Scer\GAL4^sns.PU

(Xi et al., 2024)

Detailed Description

Statement

Reference

Expression of Rab5^HMS00147 under the control of CtxGlia-SplitGal4 (Hsim\VP16^AD.nrv2 and Scer\GAL4^{DBD.wrapper.932i}) leads to an altered cortex glial morphology in third instar larvae when compared to controls.

(Coutinho-Budd et al., 2017)

Expression of Rab5^HMS00147 under the control of Scer\GAL4^{SPARC-MI00329-GAL4} results in melanisation of the fat body.

(Zang et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Rab5^HMS00147, Scer\GAL4^ninaE.PT is a suppressor | partially of decreased cell number | adult stage | progressive phenotype of Hsap\BIN1^UAS.1, Scer\GAL4^ninaE.PT

(Lambert et al., 2022)

NOT Suppressor of

Statement

Reference

Rab5^HMS00147, Scer\GAL4^ptc.PU is a non-suppressor of lethal - all die before end of P-stage phenotype of Hsap\KIF5B::Hsap\RET^UAS.cDa, Scer\GAL4^ptc.PU

(Das and Cagan, 2017)

Phenotype Manifest In

Enhanced by

Statement

Reference

Rab5^HMS00147, Scer\GAL4^sns.PU has nephrocyte | larval stage phenotype, enhanceable by cnc^JF02006, Scer\GAL4^sns.PU

(Xi et al., 2024)

Rab5^HMS00147, Scer\GAL4^sns.PU has nephrocyte diaphragm | larval stage phenotype, enhanceable by cnc^JF02006, Scer\GAL4^sns.PU

(Xi et al., 2024)

Suppressed by

Statement

Reference

Rab5^HMS00147, Scer\GAL4^sns.PU has nephrocyte | larval stage phenotype, suppressible | partially by G6pd^UAS.cLa, Scer\GAL4^sns.PU

(Xi et al., 2024)

Rab5^HMS00147, Scer\GAL4^sns.PU has nephrocyte diaphragm | larval stage phenotype, suppressible | partially by G6pd^UAS.cLa, Scer\GAL4^sns.PU

(Xi et al., 2024)

Rab5^HMS00147, Scer\GAL4^sns.PU has nephrocyte | larval stage phenotype, suppressible | partially by Cat^UAS.cAa, Scer\GAL4^sns.PU

(Xi et al., 2024)

Rab5^HMS00147, Scer\GAL4^sns.PU has nephrocyte diaphragm | larval stage phenotype, suppressible | partially by Cat^UAS.cAa, Scer\GAL4^sns.PU

(Xi et al., 2024)

Suppressor of

Statement

Reference

Rab5^HMS00147, Scer\GAL4^ninaE.PT is a suppressor | partially of outer photoreceptor cell | adult stage | decreased number phenotype of Hsap\BIN1^UAS.1, Scer\GAL4^ninaE.PT

(Lambert et al., 2022)

Rab5^HMS00147, Scer\GAL4^Cg.PA is a suppressor of fat cell | third instar larval stage phenotype of Scer\GAL4^Cg.PA, shot^GL01286

(Sun et al., 2019)

Rab5^HMS00147, Scer\GAL4^Cg.PA is a suppressor of endoplasmic reticulum | third instar larval stage phenotype of Scer\GAL4^Cg.PA, shot^GL01286

(Sun et al., 2019)

Rab5^HMS00147, Scer\GAL4^Cg.PA is a suppressor of nucleus | third instar larval stage phenotype of Scer\GAL4^Cg.PA, shot^GL01286

(Sun et al., 2019)

Other

Statement

Reference

Rab5^HMS00147, Scer\GAL4^Ugt36A1.PK, Tbc1d8-9^HA has nephrocyte phenotype

(Milosavljevic et al., 2022)

Rab5^HMS00147, Scer\GAL4^Ugt36A1.PK, Tbc1d8-9^HA has intracellular vesicle phenotype

(Milosavljevic et al., 2022)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

34832

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00147}attP2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Rab5^HMS00147

Rab5^RNAi

(Weiss et al., 2019)

Name Synonyms

Secondary FlyBase IDs

References (21)

Report Sections

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