UAS regulatory sequences drive expression of pins coding sequences where two of the GoLoco C-terminal domains are mutated (amino acid replacements R486F and R570F). The coding sequence is tagged at the N-terminal with Tag:HA.
pinsΔGL1,2.UAS.Tag:HA/Scer\GAL4wor.PA partially rescues pinsP62
Expression of rapsΔGL1,2.Scer\UAS.T:Ivir\HA1 via the neuroblast-specific Scer\GAL4wor.PA driver partially rescues the mitotic spindle orientation defect in rapsP62 mutants cells.