UAS regulatory sequences drive expression of a truncated form of Hsap\TARDBP that lacks the N-terminal 251 amino acids and in which 5 serine residues have been substituted with non-phosphorylatable alanine residues (amino acid replacements S379A, S403A, S404A, S409A and S410A). The coding sequence is tagged at the N-terminal end with EGFP.
Expression of Hsap\TARDBPS5A.ΔN251.Scer\UAS.T:Avic\GFP-EGFP after heat shock at 37[o]C under the control of Scer\GAL4Scer\FRT.Act5C results in the formation of a number of aggregates distributed within nuclear and cytoplasmic compartments (found in the salivary glands).
Expression of Hsap\TARDBPS5A.ΔN251.Scer\UAS.T:Avic\GFP-EGFP pan-neuronally under the control of Scer\GAL4elav-C155 results in the formation of aggregates in perikarya and dendrites of multi-dendritic dendrite-arborization (md-da) neurons. Most of the aggregates are located in the cytoplasmic/dendritic compartments, and few are observed in nuclei.
