Expression of Zasp52^{dsRNA.Scer\UAS} in larval muscle under the control of Scer\GAL4^how-24B does not result in any visible morphological defects in muscle structure. Electron-microscope analysis of larval muscle fibers 6 and 7 shows that silencing of Zasp52 with either Scer\GAL4^how-24B or Scer\GAL4^Act5C.PI shows that the myofibrils are less regularly and less densely packed than those of corresponding controls. The extent of such morphological alterations appears to be stronger in the case of Scer\GAL4^how-24B-driven knockdown rather than Scer\GAL4^Act5C.PI-driven knockdown.

Knockdown of Zasp52 in all tissues through Scer\GAL4^Act5C.PI-driven expression of Zasp52^{dsRNA.Scer\UAS} results in the muscle fiber 6 area becoming significantly larger than in controls. Expression of Zasp52^{dsRNA.Scer\UAS} under the control of Scer\GAL4^how-24B has no effect on muscle fiber 6 area.

Expression of Zasp52^{dsRNA.Scer\UAS} under the control of Scer\GAL4^how-24B leads to a significant decrease in the speed of locomotion and to a poorer performance in a 'roll over' test compared to controls.

Expression of Zasp52^{dsRNA.Scer\UAS} under the control of Scer\GAL4^elav-C155 leads to a weak decrease in the speed of locomotion and to a weakly poorer performance in a 'roll over' test compared to controls.

Expression of Zasp52^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Act5C.PI leads to a weak reduction in the speed of locomotion and to a significantly poorer performance in a 'roll over' test compared to controls.