FlyBase Allele Report: Dmel\E(spl)m8-HLH[123-179.CtD.UAS]

General Information

Symbol

Dmel\E(spl)m8-HLH^{123-179.CtD.UAS}

Species

D. melanogaster

Name

FlyBase ID

FBal0264411

Feature type

allele

Associated gene

Dmel\E(spl)m8-HLH

Associated Insertion(s)

Carried in Construct

P{UAS-E(spl)m8-HLH.123-179}

Key Links

Transgenic product class

inframe_deletion

(Kahali et al., 2010)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Kahali et al., 2010)

Mutagen

in vitro construct

(Kahali et al., 2010)

Progenitor genotype

Carried in construct

P{UAS-E(spl)m8-HLH.123-179}

Cytology

Description

UASt regulatory sequences drive expression of the C-terminal 123-179 residues of E(spl)m8-HLH. The phosphorylation sites in this sequence are retained and thus it should possess autoinhibitory activity.

(Kahali et al., 2010)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

E(spl)m8-HLH

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

scutellar bristle, with Scer\GAL4^455.2

(Kahali et al., 2010)

Detailed Description

Statement

Reference

Expression of E(spl)^{123-179.CtD.Scer\UAS} in the proneural clusters, under the control of Scer\GAL4^455.2 elicits ectopic macrochaeta on the scutellum of 62-78% of flies, with an average penetrance of approximately 70%.

(Kahali et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^455.2/E(spl)m8-HLH^{123-179.CtD.UAS} rescues E(spl)m8-HLH^UAS.cKa

(Kahali et al., 2010)

Scer\GAL4^sca-109-68/E(spl)m8-HLH^{123-179.CtD.UAS} rescues E(spl)m8-HLH^SD.UAS

(Kahali et al., 2010)

Comments

Co-expression of E(spl)^{123-179.CtD.Scer\UAS} under the control of Scer\GAL4^sca-109-68 strongly rescues the reduced eye found upon expression of E(spl)^SD.Scer\UAS in the developing eye morphogenetic furrow (Scer\GAL4^sca-109-68) and significantly restores the aberrant ommatidial size and patterning.

Co-expression of E(spl)^{123-179.CtD.Scer\UAS} restores 2-3 of the scutellar macrochaeta lost in a Scer\GAL4^455.2>E(spl)^Scer\UAS.cKa background. Often, socket cells and misshapen macrochaeta are found.

(Kahali et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

E(spl)^{123-179.CtD.Scer\UAS}

E(spl)m8-HLH^{123-179.CtD.Scer\UAS}

E(spl)m8-HLH^{123-179.CtD.UAS}

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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