FlyBase Allele Report: Dmel\p38b[kd.UAS.Tag:FLAG]

General Information

Symbol

Dmel\p38b^{kd.UAS.Tag:FLAG}

Species

D. melanogaster

Name

FlyBase ID

FBal0264806

Feature type

allele

Associated gene

Dmel\p38b

Associated Insertion(s)

Carried in Construct

P{UAS-p38b.kd.FLAG}

Also Known As

UAS-p38b^KD

Key Links

Transgenic product class

missense_variant

(Vrailas-Mortimer et al., 2011)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Vrailas-Mortimer et al., 2011)

Mutagen

in vitro construct

(Vrailas-Mortimer et al., 2011)

Progenitor genotype

Carried in construct

P{UAS-p38b.kd.FLAG}

Cytology

Description

UASt regulatory sequences drive expression of a mutant form of p38b in which the lysine residue at 53 has been substituted for an arginine. The construct is tagged at the C-terminal end with Tag:FLAG.

(Vrailas-Mortimer et al., 2011)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

p38b

Tagged with

Tag:FLAG

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal circadian rhythm, with Scer\GAL4^cry.PZ

(Vrailas-Mortimer et al., 2014)

abnormal circadian rhythm, with Scer\GAL4^P0.5.Pdf

(Vrailas-Mortimer et al., 2014)

abnormal locomotor behavior, with Scer\GAL4^Mef2.PR

(Vrailas-Mortimer et al., 2011)

abnormal neuroanatomy, with Scer\GAL4^cry.PZ

(Vrailas-Mortimer et al., 2014)

abnormal neuroanatomy, with Scer\GAL4^P0.5.Pdf

(Vrailas-Mortimer et al., 2014)

Phenotype Manifest In

Detailed Description

Statement

Reference

Overexpression of p38b^{kd.Scer\UAS.T:Zzzz\FLAG} in clock neurons using either Scer\GAL4^P0.5.Pdf or Scer\GAL4^cry.PZ results in a significant increase in arrhythmicity and period under free-running conditions.

(Vrailas-Mortimer et al., 2014)

Expression of p38b^{kd.Scer\UAS.T:Zzzz\FLAG} in muscles under the control of Scer\GAL4^Mef2.PR results in aberrant walking behaviour in 3 day old female flies.

(Vrailas-Mortimer et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^cry.PZ, p38b^{kd.UAS.Tag:FLAG} has abnormal circadian rhythm phenotype, enhanceable by per⁰¹/per[+]

(Vrailas-Mortimer et al., 2014)

Scer\GAL4^cry.PZ, p38b^{kd.UAS.Tag:FLAG} has abnormal neuroanatomy phenotype, enhanceable by per⁰¹/per[+]

(Vrailas-Mortimer et al., 2014)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

A per⁰¹ heterozygous background enhances the arrhythmicity and tau phenotype found upon expression of p38b^{kd.Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^cry.PZ.

(Vrailas-Mortimer et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

p38Kb^KD

(Ryan et al., 2021)

p38b^{kd.Scer\UAS.T:Zzzz\FLAG}

p38b^{kd.UAS.Tag:FLAG}

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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