FlyBase Allele Report: Dmel\Hsepi[UAS.cKa]

General Information

Symbol

Dmel\Hsepi^UAS.cKa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Kamimura

FlyBase ID

FBal0265578

Feature type

allele

Associated gene

Dmel\Hsepi

Associated Insertion(s)

Carried in Construct

P{UAS-Hsepi.K}

Key Links

Transgenic product class

cDNA

(Kamimura et al., 2011)

wild_type

(Kamimura et al., 2011)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Kamimura et al., 2011)

wild_type

(Kamimura et al., 2011)

Mutagen

in vitro construct

(Kamimura et al., 2011)

Progenitor genotype

Carried in construct

P{UAS-Hsepi.K}

Cytology

Description

UAS drives expression of a Hsepi cDNA.

(Kamimura et al., 2011)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Hsepi

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

partially lethal - majority die, with Scer\GAL4^hh.PU

(Kamimura et al., 2011)

viable, with Scer\GAL4^Act.PU

(Kamimura et al., 2011)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of Hsepi^Scer\UAS.cKa with Scer\GAL4^hh.PU results in 40% lethality.

(Kamimura et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Hsepi^UAS.cKa, Scer\GAL4^Act.PU is a suppressor of partially lethal - majority die phenotype of Scer\GAL4^Act.PU, sfl^UAS.cKa

(Kamimura et al., 2011)

Hsepi^UAS.cKa, Scer\GAL4^hh.PU is a suppressor of partially lethal - majority die phenotype of Scer\GAL4^hh.PU, sfl^UAS.cKa

(Kamimura et al., 2011)

Hsepi^UAS.cKa, Scer\GAL4^Act.PU is a suppressor of partially lethal - majority die phenotype of Hs6st^UAS.cKa, Scer\GAL4^Act.PU

(Kamimura et al., 2011)

Hsepi^UAS.cKa, Scer\GAL4^hh.PU is a suppressor of partially lethal - majority die phenotype of Hs6st^UAS.cKa, Scer\GAL4^hh.PU

(Kamimura et al., 2011)

Other

Statement

Reference

Hs2st^UAS.cKa, Hsepi^UAS.cKa, Scer\GAL4^Act.PU has viable phenotype

(Kamimura et al., 2011)

Hs2st^UAS.cKa, Hsepi^UAS.cKa, Scer\GAL4^hh.PU has viable phenotype

(Kamimura et al., 2011)

Phenotype Manifest In

Other

Statement

Reference

Hsepi^UAS.cKa, Scer\GAL4^hh.PU, sfl^UAS.cKa has anterior crossvein phenotype

(Kamimura et al., 2011)

Hsepi^UAS.cKa, Scer\GAL4^hh.PU, sfl^UAS.cKa has wing margin phenotype

(Kamimura et al., 2011)

Hs6st^UAS.cKa, Hsepi^UAS.cKa, Scer\GAL4^hh.PU has anterior crossvein phenotype

(Kamimura et al., 2011)

Hs6st^UAS.cKa, Hsepi^UAS.cKa, Scer\GAL4^hh.PU has posterior crossvein phenotype

(Kamimura et al., 2011)

Hs6st^UAS.cKa, Hsepi^UAS.cKa, Scer\GAL4^hh.PU has wing margin phenotype

(Kamimura et al., 2011)

Hs2st^UAS.cKa, Hsepi^UAS.cKa, Scer\GAL4^hh.PU has wing margin phenotype

(Kamimura et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Hsepi^Scer\UAS.cKa with sfl^Scer\UAS.cKa using Scer\GAL4^Act.PU results in 58% lethality.

Co-expression of Hsepi^Scer\UAS.cKa with Hs6st^Scer\UAS.cKa using Scer\GAL4^Act.PU results in 62% lethality.

Co-expression of Hsepi^Scer\UAS.cKa with Hs2st^Scer\UAS.cKa using Scer\GAL4^Act.PU results in 0% lethality.

Co-expression of Hsepi^Scer\UAS.cKa with sfl^Scer\UAS.cKa using Scer\GAL4^hh.PU results in 39% lethality.

Co-expression of Hsepi^Scer\UAS.cKa with Hs6st^Scer\UAS.cKa using Scer\GAL4^hh.PU results in 57% lethality.

Co-expression of Hsepi^Scer\UAS.cKa with Hs2st^Scer\UAS.cKa using Scer\GAL4^hh.PU results in 0% lethality.

Co-expression of Hsepi^Scer\UAS.cKa with sfl^Scer\UAS.cKa using Scer\GAL4^hh.PU results in posterior wing margin notching in 5% of flies and loss of the anterior crossvein in 2% of flies.

Co-expression of Hs2st^Scer\UAS.cKa with Hsepi^Scer\UAS.cKa using Scer\GAL4^hh.PU results in posterior wing margin notching in 3% of flies.

Co-expression of Hs6st^Scer\UAS.cKa with Hsepi^Scer\UAS.cKa using Scer\GAL4^hh.PU results in posterior wing margin notching in 4% of flies, loss of the anterior crossvein in 4 % of flies, and ectopic posterior cross vein tissue in 29% of flies.

(Kamimura et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Hsepi^Scer\UAS.cKa

Hsepi^UAS.cKa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Kamimura

Secondary FlyBase IDs

References (1)

Report Sections

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