FlyBase Allele Report: Dmel\sfl[UAS.cKa]

General Information

Symbol

Dmel\sfl^UAS.cKa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Kamimura

FlyBase ID

FBal0265577

Feature type

allele

Associated gene

Dmel\sfl

Associated Insertion(s)

Carried in Construct

P{UAS-sfl.K}

Key Links

Transgenic product class

cDNA

(Kamimura et al., 2011)

wild_type

(Kamimura et al., 2011)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Kamimura et al., 2011)

wild_type

(Kamimura et al., 2011)

Mutagen

in vitro construct

(Kamimura et al., 2011)

Progenitor genotype

Carried in construct

P{UAS-sfl.K}

Cytology

Description

UAS drives expression of a sfl cDNA.

(Kamimura et al., 2011)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

sfl

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size, with Scer\GAL4^hh.PU

(Kamimura et al., 2011)

partially lethal - majority die, with Scer\GAL4^Act.PU

(Kamimura et al., 2011)

partially lethal - majority die, with Scer\GAL4^hh.PU

(Kamimura et al., 2011)

Phenotype Manifest In

anterior crossvein, with Scer\GAL4^hh.PU

(Kamimura et al., 2011)

wing disc, with Scer\GAL4^hh.PU

(Kamimura et al., 2011)

wing margin, with Scer\GAL4^hh.PU

(Kamimura et al., 2011)

Detailed Description

Statement

Reference

Expression of sfl^Scer\UAS.cKa using Scer\GAL4^Act.PU does not result in any detectable apoptosis in the wing disc, eye-antennal disc or central nervous system.

Expression of sfl^Scer\UAS.cKa using Scer\GAL4^hs.PU and a 30 minute heat shock during the third larval instar stage does not result in lethality or any morphological abnormality.

Expression of sfl^Scer\UAS.cKa with Scer\GAL4^Act.PU results in 83% lethality and with Scer\GAL4^hh.PU results in 96% lethality.

Expression of sfl^Scer\UAS.cKa using Scer\GAL4^hh.PU results in posterior wing margin notching in 13% of flies and loss of the anterior crossvein in 25% of flies.

Expression of sfl^Scer\UAS.cKa using Scer\GAL4^hh.PU significantly decreases the size of the posterior compartment of the wing disc.

(Kamimura et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^Act.PU, sfl^UAS.cKa has partially lethal - majority die phenotype, suppressible by Hsepi^UAS.cKa, Scer\GAL4^Act.PU

(Kamimura et al., 2011)

Scer\GAL4^Act.PU, sfl^UAS.cKa has partially lethal - majority die phenotype, suppressible by Hs2st^UAS.cKa, Scer\GAL4^Act.PU

(Kamimura et al., 2011)

Scer\GAL4^hh.PU, sfl^UAS.cKa has partially lethal - majority die phenotype, suppressible by Hsepi^UAS.cKa, Scer\GAL4^hh.PU

(Kamimura et al., 2011)

Scer\GAL4^hh.PU, sfl^UAS.cKa has partially lethal - majority die phenotype, suppressible by Hs2st^UAS.cKa, Scer\GAL4^hh.PU

(Kamimura et al., 2011)

Other

Statement

Reference

Hs6st^UAS.cKa, Scer\GAL4^hh.PU, sfl^UAS.cKa has lethal phenotype

(Kamimura et al., 2011)

Hs6st^UAS.cKa, Scer\GAL4^hh.PU, sfl^UAS.cKa has abnormal size phenotype

(Kamimura et al., 2011)

Hs6st^UAS.cKa, Scer\GAL4^Act.PU, sfl^UAS.cKa has partially lethal - majority die phenotype

(Kamimura et al., 2011)

Phenotype Manifest In

Other

Statement

Reference

Hsepi^UAS.cKa, Scer\GAL4^hh.PU, sfl^UAS.cKa has anterior crossvein phenotype

(Kamimura et al., 2011)

Hsepi^UAS.cKa, Scer\GAL4^hh.PU, sfl^UAS.cKa has wing margin phenotype

(Kamimura et al., 2011)

Hs2st^UAS.cKa, Scer\GAL4^hh.PU, sfl^UAS.cKa has wing margin phenotype

(Kamimura et al., 2011)

Hs6st^UAS.cKa, Scer\GAL4^hh.PU, sfl^UAS.cKa has wing disc phenotype

(Kamimura et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Simultaneous expression of Hs6st^Scer\UAS.cKa and sfl^Scer\UAS.cKa using Scer\GAL4^Act.PU does not result in any detectable apoptosis in the wing disc, eye-antennal disc or central nervous system.

Simultaneous expression of Hs6st^Scer\UAS.cKa and sfl^Scer\UAS.cKa using Scer\GAL4^hs.PU and a 30 minute heat shock during the third larval instar stage does not result in lethality or any morphological abnormality.

Co-expression of Hsepi^Scer\UAS.cKa with sfl^Scer\UAS.cKa using Scer\GAL4^Act.PU results in 58% lethality.

Co-expression of Hs2st^Scer\UAS.cKa with sfl^Scer\UAS.cKa using Scer\GAL4^Act.PU results in 24% lethality.

Co-expression of Hs6st^Scer\UAS.cKa with sfl^Scer\UAS.cKa using Scer\GAL4^Act.PU results in 69% lethality.

Co-expression of Hsepi^Scer\UAS.cKa with sfl^Scer\UAS.cKa using Scer\GAL4^hh.PU results in 39% lethality.

Co-expression of Hs2st^Scer\UAS.cKa with sfl^Scer\UAS.cKa using Scer\GAL4^hh.PU results in 0% lethality.

Co-expression of Hs6st^Scer\UAS.cKa with sfl^Scer\UAS.cKa using Scer\GAL4^hh.PU results in 100% lethality.

Co-expression of Hsepi^Scer\UAS.cKa with sfl^Scer\UAS.cKa using Scer\GAL4^hh.PU results in posterior wing margin notching in 5% of flies and loss of the anterior crossvein in 2% of flies.

Co-expression of Hs2st^Scer\UAS.cKa with sfl^Scer\UAS.cKa using Scer\GAL4^hh.PU results in posterior wing margin notching in 3% of flies.

Co-expression of sfl^Scer\UAS.cKa and Hs6st^Scer\UAS.cKa using Scer\GAL4^hh.PU significantly decreases the size of the posterior compartment of the wing disc.

(Kamimura et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

sfl^Scer\UAS.cKa

sfl^UAS.cKa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Kamimura

Secondary FlyBase IDs

References (1)

Report Sections

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