FlyBase Allele Report: Dmel\Ran[UASp.Tag:HA]

General Information

Symbol

Dmel\Ran^UASp.Tag:HA

Species

D. melanogaster

Name

FlyBase ID

FBal0265625

Feature type

allele

Associated gene

Dmel\Ran

Associated Insertion(s)

Carried in Construct

P{UASp-Ran.C}

Key Links

Transgenic product class

wild_type

(Cesario and McKim, 2011)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Cesario and McKim, 2011)

Mutagen

in vitro construct

(Cesario and McKim, 2011)

Progenitor genotype

Carried in construct

P{UASp-Ran.C}

Cytology

Description

UASp regulatory sequences drive expression of wild-type Ran which is tagged at the N-terminal end with three copies of Tag:HA.

(Cesario and McKim, 2011)

Allele components

Component

Use(s)

Regulatory region(s)

UASp

binary expression system - regulatory region

Encoded product / tool

Ran

Tagged with

Tag:HA

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Huntington's disease

modeled by Hsap\HTT^{N548.Q128.UAS}

(Grima et al., 2017)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

fertile, with Scer\GAL4^{VP16.nanos.UTR}

(Cesario and McKim, 2011)

viable, with Scer\GAL4^αTub84B.PL

(Cesario and McKim, 2011)

Phenotype Manifest In

Detailed Description

Statement

Reference

Spindle and karyosome morphology appears normal in oocytes expressing ran^{Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}.

Zygotes derived from females expressing ran^{Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} undergo normal synchronous divisions, with wild-type spindle assembly and chromosome organisation.

(Cesario and McKim, 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Suppressor of

Statement

Reference

Ran^UASp.Tag:HA, Scer\GAL4^{VP16.nanos.UTR} is a non-suppressor of female sterile phenotype of Scer\GAL4^{VP16.nanos.UTR}, sub^{ΔNT.UASp.EGFP}

(Cesario and McKim, 2011)

Phenotype Manifest In

NOT Suppressor of

Statement

Reference

Ran^UASp.Tag:HA, Scer\GAL4^{VP16.nanos.UTR} is a non-suppressor of spindle | ectopic phenotype of Scer\GAL4^{VP16.nanos.UTR}, sub^{ΔNT.UASp.EGFP}

(Cesario and McKim, 2011)

Ran^UASp.Tag:HA, Scer\GAL4^{VP16.nanos.UTR} is a non-suppressor of oocyte phenotype of Scer\GAL4^{VP16.nanos.UTR}, sub^{ΔNT.UASp.EGFP}

(Cesario and McKim, 2011)

Additional Comments

Genetic Interactions

Statement

Reference

The frequency of the ectopic spindle phenotype seen in the oocytes of females expressing sub^{ΔNT.Scer\UAS.P\T.T:Avic-EGFP} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} is not suppressed by co-expression of ran^{Scer\UAS.P\T.T:Ivir\HA1}.

(Cesario and McKim, 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Ran^UASp.Tag:HA/Scer\GAL4^αTub84B.PL rescues Ran^G0075

(Cesario and McKim, 2011)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Ran^{Scer\UAS.P\T.T:Ivir\HA1}

Ran^UASp.Tag:HA

ran^{Scer\UAS.P\T.T:Ivir\HA1}

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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