FlyBase Allele Report: Dmel\Ran[T24N.UASp.Tag:HA]

General Information

Symbol

Dmel\Ran^{T24N.UASp.Tag:HA}

Species

D. melanogaster

Name

FlyBase ID

FBal0265626

Feature type

allele

Associated gene

Dmel\Ran

Associated Insertion(s)

Carried in Construct

P{UASp-Ran.T24N}

Key Links

Transgenic product class

missense_variant

(Cesario and McKim, 2011)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Cesario and McKim, 2011)

Mutagen

in vitro construct

(Cesario and McKim, 2011)

Progenitor genotype

Carried in construct

P{UASp-Ran.T24N}

Cytology

Description

UASp regulatory sequences drive expression of a GDP-locked form of Ran (amino acid replacement T24N) which is tagged at the N-terminal end with three copies of Tag:HA.

(Cesario and McKim, 2011)

Allele components

Component

Use(s)

Regulatory region(s)

UASp

binary expression system - regulatory region

Encoded product / tool

Ran

Tagged with

Tag:HA

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates amyotrophic lateral sclerosis

modeled by Zzzz\GGGGCC^58.UAS.EGFP

(Freibaum et al., 2015)

exacerbates frontotemporal dementia

modeled by Zzzz\GGGGCC^58.UAS.EGFP

(Freibaum et al., 2015)

exacerbates Huntington's disease

modeled by Hsap\HTT^{N548.Q128.UAS}

(Grima et al., 2017)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female sterile, with Scer\GAL4^{VP16.nanos.UTR}

(Cesario and McKim, 2011)

lethal, with Scer\GAL4^αTub84B.PL

(Cesario and McKim, 2011)

male sterile, with Scer\GAL4^{VP16.nanos.UTR}

(Cesario and McKim, 2011)

visible

(Freibaum et al., 2015)

Phenotype Manifest In

annulate lamellae | oogenesis, with Scer\GAL4^{VP16.nanos.UTR}

(Hampoelz et al., 2019)

eye

(Freibaum et al., 2015)

karyosome, with Scer\GAL4^{VP16.nanos.UTR}

(Cesario and McKim, 2011)

meiotic spindle, with Scer\GAL4^{VP16.nanos.UTR}

(Cesario and McKim, 2011)

oocyte, with Scer\GAL4^{VP16.nanos.UTR}

(Hampoelz et al., 2019)

Detailed Description

Statement

Reference

The expression of Ran^{T24N.UASp.Tag:HA} under the control of Scer\GAL4^VP16.nos.UTR leads to fewer annulate lamellae in the ooplasm.

(Hampoelz et al., 2019)

Ran^{T24N.Scer\UAS.P\T.T:Ivir\HA1} causes a rough eye phenotype.

(Freibaum et al., 2015)

Expression of ran^{T24N.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} results in a drastic reduction in female fertility, with an average of only 6.8 progeny per female (compared to 62.1 per wild-type female). Males expressing ran^{T24N.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} are completely sterile.

Defects in spindle and karyosome morphology are often seen in oocytes expressing ran^{T24N.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}. The microtubules are often not tapered at the spindle poles and the chromosomes are often disorganised and fail to condense into a single round or oval karyosome.

Chiasmate chromosome segregation is not affected in females expressing ran^{T24N.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}. However, the segregation of achiasmate chromosomes is disrupted in these animals.

The majority of zygotes derived from females expressing ran^{T24N.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} arrest development without any evidence of embryonic mitotic divisions. Two types of zygote are seen. Approximately half contain the unfused female and male meiotic products and lack organised microtubules, while the remaining half have no visible nuclei.

(Cesario and McKim, 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Ran^{T24N.UASp.Tag:HA}, Scer\GAL4^{VP16.nanos.UTR} has female sterile phenotype, suppressible by sub^{ΔNT.UASp.EGFP}, Scer\GAL4^{VP16.nanos.UTR}

(Cesario and McKim, 2011)

Enhancer of

Statement

Reference

Ran^{T24N.UASp.Tag:HA} is an enhancer of visible phenotype of Scer\GAL4^GMR.PU, Zzzz\GGGGCC^58.UAS.EGFP

(Freibaum et al., 2015)

Suppressor of

Statement

Reference

Ran^{T24N.UASp.Tag:HA}, Scer\GAL4^{VP16.nanos.UTR} is a suppressor | partially of female sterile phenotype of Scer\GAL4^{VP16.nanos.UTR}, sub^{ΔNT.UASp.EGFP}

(Cesario and McKim, 2011)

Phenotype Manifest In

Enhancer of

Statement

Reference

Ran^{T24N.UASp.Tag:HA} is an enhancer of eye phenotype of Scer\GAL4^GMR.PU, Zzzz\GGGGCC^58.UAS.EGFP

(Freibaum et al., 2015)

Suppressor of

Statement

Reference

Ran^{T24N.UASp.Tag:HA}, Scer\GAL4^{VP16.nanos.UTR} is a suppressor of spindle | ectopic phenotype of Scer\GAL4^{VP16.nanos.UTR}, sub^{ΔNT.UASp.EGFP}

(Cesario and McKim, 2011)

Ran^{T24N.UASp.Tag:HA}, Scer\GAL4^{VP16.nanos.UTR} is a suppressor of oocyte phenotype of Scer\GAL4^{VP16.nanos.UTR}, sub^{ΔNT.UASp.EGFP}

(Cesario and McKim, 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of ran^{T24N.Scer\UAS.P\T.T:Ivir\HA1} and sub^{ΔNT.Scer\UAS.P\T.T:Avic-EGFP} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} increases female fertility compared to the fertility of females expressing either ran^{T24N.Scer\UAS.P\T.T:Ivir\HA1} or sub^{ΔNT.Scer\UAS.P\T.T:Avic-EGFP} separately.

The ectopic spindle phenotype seen in the oocytes of females expressing sub^{ΔNT.Scer\UAS.P\T.T:Avic-EGFP} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} is completely suppressed by co-expression of ran^{T24N.Scer\UAS.P\T.T:Ivir\HA1}.

The arrest in embryonic development seen in zygotes derived from females expressing ran^{T24N.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} is partially suppressed by co-expression of sub^{ΔNT.Scer\UAS.P\T.T:Avic-EGFP}.

(Cesario and McKim, 2011)

Xenogenetic Interactions

Statement

Reference

Presence of the Ran^{T24N.Scer\UAS.P\T.T:Ivir\HA1} mutation enhances the rough eye phenotype seen in flies expressing Zzzz\GGGGCC^{58.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^GMR.PU.

(Freibaum et al., 2015)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Ran^{T24N.Scer\UAS.P\T.T:Ivir\HA1}

Ran^{T24N.UASp.Tag:HA}

ran^{T24N.Scer\UAS.P\T.T:Ivir\HA1}

ran^T24N

(Cesario and McKim, 2011)

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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