FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\pzg66
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General Information
Symbol
Dmel\pzg66
Species
D. melanogaster
Name
FlyBase ID
FBal0265712
Feature type
allele
Associated gene
Dmel\pzg
Associated Insertion(s)
P{3'SUPor-P}pzg66
Carried in Construct
Key Links
Genomic Maps

Allele class

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - molecular evidence

(Kugler et al., 2011)
Mutagen
Delta2-3 transposase
(Kugler et al., 2011)
Progenitor genotype
P{SUPor-P}KG04911
(Kugler et al., 2011)
Associated Insertion(s)
P{3'SUPor-P}pzg66
Cytology
Description

Imprecise excision of the progenitor insertion, resulting in a 7083bp deletion within the inserted P{SUPor-P} element and a deletion of 839bp of pzg sequences, including the transcription and translation start sites. A 3' part of the original P{SUPor-P} insertion remains.

(Kugler et al., 2011)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
deletion
3L:21,286,043..21,286,881 [+]
Comment:

A deletion resulting from the imprecise excision of P{SUPor-P}KG04911 which removes7083 bp within the P-element and 839 bp of pzg sequences.

(Kugler et al., 2011)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Homozygous embryos show normal development. Homozygous larvae show a developmental delay and are smaller and thinner than wild-type larvae. The mutant larvae show an almost linear mortality rate with age, and none of them survive more than 150 hours. They moult only once during this time, reaching the second instar stage, but then there is no further increase in size. The addition of 20-hydroxyecdysone to the diet results in almost 90% of mutants surviving to to the second instar and nearly all of them reach the third instar.

      Homozygous first and second instar larvae show significantly reduced mouth-hook contractions per time interval relative to controls. They are able to take up food, as assayed by the presence of coloured yeast in the gut in a feeding assay. Homozygous larvae are more sluggish than control larvae and remain dispersed on the plates in the feeding assay, in contrast to wild-type larvae which reach the central food source more quickly.

      (Kugler et al., 2011)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhancer of
      Statement
      Reference

      pzg66/pzg[+] is an enhancer of melanotic mass phenotype | adult stage | dominant phenotype of hopTum

      (Kugler et al., 2011)
      Phenotype Manifest In
      Enhancer of
      Statement
      Reference

      pzg66/pzg[+] is an enhancer of melanotic mass | adult stage phenotype of hopTum

      (Kugler et al., 2011)

      pzg66/pzg[+] is an enhancer of lamellocyte | larval stage | increased number phenotype of hopTum

      (Kugler et al., 2011)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The penetrance of the melanotic mass phenotype seen in hopTum/+ females is enhanced if they also carry one copy of pzg66.

      The incidence of lamellocytes as a percentage of the total hemocytes in hemolymph preparations is increased in hopTum/+ ; pzg66/+ larvae compared to hopTum/+ larvae.

      (Kugler et al., 2011)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      pzg66 is rescued by Scer\GAL4da.G32/pzgUAS.cKa

      (Kugler et al., 2011)
      Comments

      Expression of pzgScer\UAS.cKa under the control of Scer\GAL4da.G32 rescues the lethality of homozygous pzg66 larvae, in a dose-dependent manner.

      (Kugler et al., 2011)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      pzg66
      (Li et al., 2017, Kugler et al., 2011)
      Name Synonyms
      Secondary FlyBase IDs
        References (2)