FlyBase Allele Report: Dmel\Lapsyn[zg1]

General Information

Symbol

Dmel\Lapsyn^zg1

Species

D. melanogaster

Name

FlyBase ID

FBal0265770

Feature type

allele

Associated gene

Dmel\Lapsyn

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Richier et al., 2017)

Mutagen

P-element activity

(Guan et al., 2011)

Progenitor genotype

P{SUPor-P}Lapsyn^KG09421

(Guan et al., 2011)

Cytology

Description

A deletion removing the 5' UTR and the first two coding exons of Lapsyn resulting from the imprecise excision of P{SUPor-P}CG15658^KG09421.

(Guan et al., 2011)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2R:21,193,461..21,198,205 [+]

Comment:

Approximate boundaries of a deletion resulting from the imprecise excision of P{SUPor-P}Lapsyn^KG09421, which extends from the insertion site into the Lapsyn gene, removing the first two coding exons.

(Guan et al., 2011)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal learning | dominant | third instar larval stage

(Guan et al., 2011)

abnormal learning | third instar larval stage, with Lapsyn^UAS.cGa, Scer\GAL4^elav-C155

(Guan et al., 2011)

abnormal neuroanatomy | dominant | third instar larval stage

(Guan et al., 2011)

lethal - all die before end of P-stage, with Lapsyn^UAS.cGa, Scer\GAL4^elav-C155

(Guan et al., 2011)

lethal - all die during embryonic stage, with Lapsyn^UAS.cGa, Scer\GAL4^Mhc.PU

(Guan et al., 2011)

lethal - all die during embryonic stage (with Df(2R)Exel6072)

(Guan et al., 2011)

lethal - all die during embryonic stage | recessive

(Guan et al., 2011)

some die during embryonic stage, with Lapsyn^UAS.cGa, Scer\GAL4^Mhc.PU

(Guan et al., 2011)

some die during embryonic stage (with Df(2R)Exel6072)

(Guan et al., 2011)

some die during embryonic stage | recessive

(Guan et al., 2011)

some die during P-stage, with Lapsyn^UAS.cGa, Scer\GAL4^elav-C155

(Guan et al., 2011)

Phenotype Manifest In

NMJ bouton | increased number | third instar larval stage

(Guan et al., 2011)

synapse | third instar larval stage

(Guan et al., 2011)

Detailed Description

Statement

Reference

Homozygous Lapsyn^zg1 mutants do not show any obvious developmental defects in the formation of the central (CNS) or peripheral (PNS) nervous systems. CNS commissure formation and PNS axonal path-finding both appear normal in the mutants.

Heterozygous Lapsyn^zg1 third instar larvae display abnormal synaptic structure with supernumerary satellite boutons at the neuromuscular junction.

Heterozygous Lapsyn^zg1 larvae show normal olfactory and gustatory behaviours. In contrast to wild-type, associative learning in groups of reciprocally trained Lapsyn^zg1 heterozygotes is absent.

(Guan et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Lapsyn^zg1 has abnormal neuroanatomy | dominant | third instar larval stage phenotype, suppressible | partially by Adcy1^rut-1

(Guan et al., 2011)

Phenotype Manifest In

Suppressed by

Statement

Reference

Lapsyn^zg1 has NMJ bouton | third instar larval stage phenotype, suppressible | partially by Adcy1^rut-1

(Guan et al., 2011)

Lapsyn^zg1 has synapse | third instar larval stage phenotype, suppressible | partially by Adcy1^rut-1

(Guan et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

The increase in satellite bouton formation observed in Lapsyn^zg1 heterozygotes is partially suppressed in a rut¹ mutant background.

(Guan et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

Df(2R)Exel6072/Lapsyn^zg1 is partially rescued by Scer\GAL4^Act.PU/Lapsyn^UAS.cGa

(Guan et al., 2011)

Lapsyn^zg1 is partially rescued by Scer\GAL4^elav-C155/Lapsyn^UAS.cGa

(Guan et al., 2011)

Lapsyn^zg1 is partially rescued by Scer\GAL4^Act.PU/Lapsyn^UAS.cGa

(Guan et al., 2011)

Lapsyn^zg1 is partially rescued by Lapsyn^UAS.cGa/Scer\GAL4^αTub84B.PL

(Guan et al., 2011)

[+]/Lapsyn^zg1 is partially rescued by Scer\GAL4^elav-C155/Lapsyn^UAS.cGa

(Guan et al., 2011)

Not rescued by

Lapsyn^zg1 is not rescued by Lapsyn^UAS.cGa/Scer\GAL4^Mhc.PU

(Guan et al., 2011)

Comments

Overexpression of Lapsyn^Scer\UAS.cGa under the control of Scer\GAL4^Act.PU partially rescues the lethality of both Lapsyn^zg1/Df(2R)Exel6072 and homozygous Lapsyn^zg1 mutants. The rescued homozygous Lapsyn^zg1 animals emerge as adults and show no obvious behavioural abnormalities. Similar results are obtained using Scer\GAL4^αTub84B.PL.

Overexpression of Lapsyn^Scer\UAS.cGa under the control of Scer\GAL4^elav-C155 partially rescues the lethality of homozygous Lapsyn^zg1. The rescued animals survive to the pupal stage, but they fail to eclose as adults.

Overexpression of Lapsyn^Scer\UAS.cGa via Scer\GAL4^Mhc.PU fails to rescue the lethality of homozygous Lapsyn^zg1. Homozygous Lapsyn^zg1 mutants expressing Lapsyn^Scer\UAS.cGa under the control of Scer\GAL4^Mhc.PU die as embryos.

Presynaptic expression of Lapsyn^Scer\UAS.cGa driven by Scer\GAL4^elav-C155 can rescue the satellite bouton phenotype of heterozygous Lapsyn^zg1. However, expression of Lapsyn^Scer\UAS.cGa under the control of Scer\GAL4^elav-C155 fails to rescue the learning defects in heterozygous Lapsyn^zg1 larvae.

(Guan et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Lapsyn^zg1

(Guan et al., 2011)

lapsyn^ZG1

(Richier et al., 2017)

Name Synonyms

Secondary FlyBase IDs

References (2)

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