FB2026_02 , released June 18, 2026
FB2026_02 , released June 18, 2026
Allele: Dmel\MRG15RNAi.tetO
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General Information
Symbol
Dmel\MRG15RNAi.tetO
Species
D. melanogaster
Name
FlyBase ID
FBal0265940
Feature type
allele
Associated gene
Dmel\MRG15
Associated Insertion(s)
Carried in Construct
P{tetO-MRG15.RNAi}
Key Links
Transgenic product class
RNAi_reagent
(Zhang et al., 2010)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Zhang et al., 2010)
Mutagen
in vitro construct
(Zhang et al., 2010)
Progenitor genotype
Carried in construct
P{tetO-MRG15.RNAi}
Cytology
Description

tetO regulatory sequences drive expression of two copies of MRG15 coding sequences from +314 to +1272bp oriented in an inverted repeat that forms a dsRNA hairpin when expressed.

(Zhang et al., 2010)
Allele components
Component
Use(s)
Regulatory region(s)
tetO
Encoded product / tool
MRG15
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Induction of P{tetO-MRG15.dsRNA}4-1 through the presence of doxycycline and Tn10\tetRAct5C.T:Hsim\VP16 decreases male life span by -7%, line P{tetO-MRG15.dsRNA}4-3 by -10% and line P{tetO-MRG15.dsRNA}53 by -7.32%. In contrast, the effects on female life span are generally smaller and less consistently observed: line P{tetO-MRG15.dsRNA}4-1 decreases female life span by -8%, line P{tetO-MRG15.dsRNA}4-3 decreases female life span by -16.7%, while line P{tetO-MRG15.dsRNA}53 causes a small decrease in female life span.

      Induction of P{tetO-MRG15.dsRNA}4-1 through the presence of doxycycline and Tn10\tetRAct5C.T:Hsim\VP16 decreases female fertility by -50%, while line P{tetO-MRG15.dsRNA}53 decreases female fertility by -72% and line P{tetO-MRG15.dsRNA}4-3 by -45%.

      Induction of P{tetO-MRG15.dsRNA}4-1 through the presence of doxycycline and Tn10\tetRAct5C.T:Hsim\VP16 decreases the survival rate of male larvae by 9% and female larvae by 13.5%. Induction of P{tetO-MRG15.dsRNA}4-3 decreases larval survival by 4.4% in males and 10.2% in females, whereas line P{tetO-MRG15.dsRNA}53 decreases male survival by 24.5% with the female survival decrease being statistically insignificant.

      (Zhang et al., 2010)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      MRG15RNAi.tetO
      MRG15dsRNA.Tn10\tetO
      MRG15dsRNA.tetO
      Name Synonyms
      Secondary FlyBase IDs
        References (2)