FlyBase Allele Report: Tn10\tetR[rtTA.Act5C]

General Information

Symbol

Tn10\tetR^rtTA.Act5C

Species

b. Transposon Tn10

Name

FlyBase ID

FBal0091143

Feature type

allele

Associated gene

Tn10\tetR

Associated Insertion(s)

Carried in Construct

P{rtTA.B}

Also Known As

rtTA

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Ryu et al., 2001, Bieschke et al., 1998)

Progenitor genotype

Carried in construct

P{rtTA.B}

Cytology

Description

The constitutive Act5C promoter and 5' untranslated region regulate expression of the rtTA driver.

(Bieschke et al., 1998)

Allele components

Component

Use(s)

Regulatory region(s)

Act5C

Encoded product / tool

rtTA

binary expression system - small molecule-regulated driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

organism | ubiquitous

(Bieschke et al., 1998)

adult stage

organism | ubiquitous

(Bieschke et al., 1998)

somatic cell

(Khokhar et al., 2008)

adult stage | female

ovary

(Khokhar et al., 2008)

follicle cell

(Khokhar et al., 2008)

Additional Information

Statement

Reference

Tn10 etR^rtTA.Act5C drives tetracycline-inducible expression in all somatic cells. Expression is also induced by tetracycline derivatives such as doxycycline.

(Khokhar et al., 2008)

Tn10 etR^rtTA.Act5C drives tetracycline-inducible expression ubiquitously. Expression is also induced by tetracycline derivatives such as doxycycline.

(Bieschke et al., 1998)

Marker for

Reflects expression of

Act5C

(Khokhar et al., 2008, Bieschke et al., 1998)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal heat stress response | tetracycline conditional, with Hsp22^tetO.cBa

(Bhole et al., 2004)

abnormal oxidative stress response | tetracycline conditional, with Hsp22^tetO.cBa

(Bhole et al., 2004)

female semi-sterile, with MRG15^{MT1.tetO.Tag:HA}

(Zhang et al., 2010)

female semi-sterile, with MRG15^RNAi.tetO

(Zhang et al., 2010)

increased cell death | P-stage, with Hsap\UBB^tetO.cHa

(Hoe et al., 2011)

lethal, with PdL3A^PdL3A

(Landis et al., 2001)

lethal, with PdL45C1^PdL45C1

(Landis et al., 2001)

lethal, with PdL72A3^PdL72A3

(Landis et al., 2001)

lethal, with PdL75A3^PdL75A3

(Landis et al., 2001)

lethal | RU486 conditional, with ash1^PdL11A3

(Landis et al., 2001)

lethal | RU486 conditional, with PdL2B1^PdL2B1

(Landis et al., 2001)

lethal | RU486 conditional, with PdL14B1^PdL14B1

(Landis et al., 2001)

lethal | RU486 conditional, with PdL43C1^PdL43C1

(Landis et al., 2001)

lethal | RU486 conditional, with PdL73A1^PdL73A1

(Landis et al., 2001)

lethal - all die before end of P-stage, with Hsap\UBB^tetO.cHa

(Hoe et al., 2011)

long lived, with cher^PdL8S64

(Landis et al., 2003)

long lived, with fwd^PdL8S25

(Landis et al., 2003)

long lived, with Pcyt1^PdL8R128

(Landis et al., 2003)

long lived, with Rdh^PdL3E36

(Landis et al., 2003)

long lived, with Sod2^tetO.cCa

(Curtis et al., 2007)

long lived, with Sugb^PdL2C33

(Landis et al., 2003)

long lived, with VhaSFD^PdL4G14

(Landis et al., 2003)

long lived | RU486 conditional, with PdL73A1^PdL73A1

(Landis et al., 2001)

long lived | RU486 conditional, with α-Man-IIa^PdL19B3

(Landis et al., 2001)

melanotic mass phenotype | P-stage, with Hsap\UBB^tetO.cHa

(Hoe et al., 2011)

short lived, with MRG15^RNAi.tetO

(Zhang et al., 2010)

short lived, with PdL14B1^PdL14B1

(Landis et al., 2001)

short lived | :heat sensitive | tetracycline conditional, with Hsp22^tetO.cBa

(Bhole et al., 2004)

short lived | heat sensitive, with Sod2^tetO.cCa

(Curtis et al., 2007)

short lived | RU486 conditional, with ash1^PdL11A3

(Landis et al., 2001)

short lived | RU486 conditional, with PdL2B1^PdL2B1

(Landis et al., 2001)

short lived | RU486 conditional, with PdL14B1^PdL14B1

(Landis et al., 2001)

short lived | RU486 conditional, with PdL35B1^PdL35B1

(Landis et al., 2001)

short lived | RU486 conditional, with PdL43C1^PdL43C1

(Landis et al., 2001)

short lived | RU486 conditional, with pum^PdL58A2

(Landis et al., 2001)

short lived | tetracycline conditional, with Sod2^tetO.cFa

(Ford et al., 2007)

some die during P-stage, with Hsap\UBB^tetO.cHa

(Hoe et al., 2011)

visible, with pum^PdL3B2

(Landis et al., 2001)

visible, with pum^PdL3G

(Landis et al., 2001)

visible | adult stage | RU486 conditional, with α-Man-IIa^PdL19B3

(Landis et al., 2001)

visible | RU486 conditional, with PdL35B1^PdL35B1

(Landis et al., 2001)

visible | RU486 conditional, with pum^PdL58A2

(Landis et al., 2001)

Phenotype Manifest In

dorsal appendage | drug conditional, with dbr^3M1753

(Khokhar et al., 2008)

dorsal appendage | drug conditional, with edl^4M127

(Khokhar et al., 2008)

dorsal appendage | drug conditional, with foxo^Y2-26

(Khokhar et al., 2008)

dorsal appendage | drug conditional, with wmd^pter-2-4

(Khokhar et al., 2008)

egg chorion | drug conditional, with foxo^Y2-26

(Khokhar et al., 2008)

egg chorion | drug conditional, with ush^3-38

(Khokhar et al., 2008)

eye | RU486 conditional, with α-Man-IIa^PdL19B3

(Landis et al., 2001)

micropyle | drug conditional, with dbr^3M1753

(Khokhar et al., 2008)

pupa, with Hsap\UBB^tetO.cHa

(Hoe et al., 2011)

wing, with pum^PdL3B2

(Landis et al., 2001)

wing, with pum^PdL3G

(Landis et al., 2001)

wing | RU486 conditional, with PdL35B1^PdL35B1

(Landis et al., 2001)

wing | RU486 conditional, with pum^PdL58A2

(Landis et al., 2001)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Product binds DNA only in the presence of tetracycline.

(Bieschke et al., 1998)

Carried in a plasmid and transfected into S2 cells.

(Ryu et al., 2001)

Carried in plasmid "Tet-VP16", transfected into S2 cells and used in transcription assays.

(Ryu and Arnosti, 2003)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

Tet-VP16

(Ryu et al., 2001)

Tn10\tetR^{Act5C.T:Hsim\VP16}

Tn10\tetR^rtTA.Act5C

pSC101\tetR^{Act5C.T:Hsim\VP16}

rtTA

(Bieschke et al., 1998)

tetR::VP16^Act5C.PB

tetR^{Act5C.T:Hsim\VP16}

Name Synonyms

reverse tetracycline transactivator

(Bieschke et al., 1998)

Secondary FlyBase IDs

References (14)

Report Sections

Open Close