FB2026_02 , released June 18, 2026
Recombinant Construct: P{rtTA.B}
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General Information
Symbol
P{rtTA.B}
FlyBase ID
FBtp0009645
Feature type
transgenic_transposable_element
Component Allele(s)
Tn10\tetRrtTA.Act5C
Also Known As
rtTA
Size
Expression Data
Associated insertion(s)
10 available
Molecular map
Description and Uses
Construct components
Component
Use(s)
Marker(s)
w+mC
Component allele Tn10\tetRrtTA.Act5C
Transgenic product class
Component
Use(s)
Regulatory region(s)
Act5C
Encoded product / tool
rtTA
Description

The constitutive Act5C promoter and 5' untranslated region regulate expression of the rtTA driver.

(Bieschke et al., 1998)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
larval stage
organism | ubiquitous
(Bieschke et al., 1998)
adult stage
organism | ubiquitous
(Bieschke et al., 1998)
somatic cell
(Khokhar et al., 2008)
adult stage | female
ovary
(Khokhar et al., 2008)
follicle cell
(Khokhar et al., 2008)
Additional Information
Statement
Reference

Tn10 etRrtTA.Act5C drives tetracycline-inducible expression in all somatic cells. Expression is also induced by tetracycline derivatives such as doxycycline.

(Khokhar et al., 2008)

Tn10 etRrtTA.Act5C drives tetracycline-inducible expression ubiquitously. Expression is also induced by tetracycline derivatives such as doxycycline.

(Bieschke et al., 1998)
 
Marker for
Reflects expression of
Act5C
(Khokhar et al., 2008, Bieschke et al., 1998)
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments

construct_comment: Adaptation of rtTA (Tn10\tetR- T:Hsim \VP16 transactivator fusion protein) to Drosophila by use of Act5C promoter; used to test efficacy of a tetR-tetO binary system in Drosophila.

(Bieschke et al., 1998)
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
References (13)