FlyBase Gene Report: Tn10\tetR

FB2026_01 , released March 12, 2026

FB2026_01 , released March 12, 2026

Gene: Tn10\tetR

General Information

Symbol

Tn10\tetR

Species

b. Transposon Tn10

Name

tetracyclin repressor

Annotation Symbol

Feature Type

engineered_foreign_gene

FlyBase ID

FBgn0016944

Gene Model Status

Stock Availability

6 publicly available

Gene Summary

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Interactive Fly

Also Known As

tTA, rtTA, tetR

Foreign Gene Data

Foreign sequence; species == bacterial transposon; gene == 'tetR, tetracyclin repressor'; EMBL:X00694 .

(FlyBase Curators, 1992-)

Summaries

Alleles, Insertions, Transgenic Constructs

Classical and Insertion Alleles ( 0 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 29 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Tn10\tetR

Transgenic constructs containing regulatory region of Tn10\tetR

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with CaMKII^T287D.tetO, Scer\GAL4^29BD

Tn10\tetR^tTA.UAS

lethal, with CaMKII^T287D.tetO, Scer\GAL4^elav-C155

Tn10\tetR^tTA.UAS

lethal, with CaMKII^T287D.tetO, Scer\GAL4^MJ85b

Tn10\tetR^tTA.UAS

lethal, with PdL3A^PdL3A

Tn10\tetR^rtTA.Act5C

lethal, with PdL45C1^PdL45C1

Tn10\tetR^rtTA.Act5C

lethal, with PdL72A3^PdL72A3

Tn10\tetR^rtTA.Act5C

lethal, with PdL75A3^PdL75A3

Tn10\tetR^rtTA.Act5C

lethal, with Ras64B^V12.tetO

Tn10\tetR^tTA.Hsp26

lethal | female, with msl-2^NOPU.tetO

lethal | female, with Ras64B^V12.tetO

Tn10\tetR^tTA.Yp3

lethal | female limited, with hid^tetO.cHa

Tn10\tetR^tTA.Yp1

lethal | RU486 conditional, with ash1^PdL11A3

Tn10\tetR^rtTA.Act5C

lethal | RU486 conditional, with PdL2B1^PdL2B1

Tn10\tetR^rtTA.Act5C

lethal | RU486 conditional, with PdL14B1^PdL14B1

Tn10\tetR^rtTA.Act5C

lethal | RU486 conditional, with PdL43C1^PdL43C1

Tn10\tetR^rtTA.Act5C

lethal | RU486 conditional, with PdL73A1^PdL73A1

Tn10\tetR^rtTA.Act5C

lethal | tetracycline conditional, with Scer\GAL4^salm-459.2, rpr^tetO.cSa

Tn10\tetR^{rtTA(M2-alt).UAS}

lethal - all die before end of P-stage, with Hsap\UBB^tetO.cHa

Tn10\tetR^rtTA.Act5C

Sterility

Allele

female semi-sterile, with MRG15^{MT1.tetO.Tag:HA}

Tn10\tetR^rtTA.Act5C

female semi-sterile, with MRG15^RNAi.tetO

Tn10\tetR^rtTA.Act5C

Other Phenotypes

Allele

abnormal courtship behavior, with CaMKII^T287D.tetO, Scer\GAL4^30Y

Tn10\tetR^tTA.UAS

abnormal courtship behavior, with CaMKII^T287D.tetO, Scer\GAL4^MJ85b

Tn10\tetR^tTA.UAS

abnormal courtship behavior | male | tetracycline conditional, with CaMKII^T287D.tetO, Scer\GAL4^30Y

Tn10\tetR^tTA.UAS

abnormal courtship behavior | male | tetracycline conditional, with CaMKII^T287D.tetO, Scer\GAL4^30Y, Scer\GAL80^ChAT.3.3P

Tn10\tetR^tTA.UAS

abnormal heat stress response | tetracycline conditional, with Hsp22^tetO.cBa

Tn10\tetR^rtTA.Act5C

abnormal neurophysiology | drug conditional, with Bper\ptxA^tetO.cCa, Scer\GAL4^{Orco.2.642.Hsim\VP22}

Tn10\tetR^{rtTA(M2-alt).UAS}

abnormal oxidative stress response | tetracycline conditional, with Hsp22^tetO.cBa

Tn10\tetR^rtTA.Act5C

abnormal smell perception | drug conditional, with Bper\ptxA^tetO.cCa, Scer\GAL4^{Orco.2.642.Hsim\VP22}

Tn10\tetR^{rtTA(M2-alt).UAS}

decreased body size, with CaMKII^T287D.tetO, Scer\GAL4^MJ85b

Tn10\tetR^tTA.UAS

increased cell death | P-stage, with Hsap\UBB^tetO.cHa

Tn10\tetR^rtTA.Act5C

long lived, with cher^PdL8S64

Tn10\tetR^rtTA.Act5C

long lived, with fwd^PdL8S25

Tn10\tetR^rtTA.Act5C

long lived, with Pcyt1^PdL8R128

Tn10\tetR^rtTA.Act5C

long lived, with Rdh^PdL3E36

Tn10\tetR^rtTA.Act5C

long lived, with Scer\GAL4^Toll-6-D42, hebe^tetO.cLa

Tn10\tetR^{rtTA(M2-alt).UAS}

long lived, with Scer\GAL4^Toll-6-D42, magu^tetO.cLa

Tn10\tetR^{rtTA(M2-alt).UAS}

long lived, with Sod2^tetO.cCa

Tn10\tetR^rtTA.Act5C

long lived, with Sugb^PdL2C33

Tn10\tetR^rtTA.Act5C

long lived, with VhaSFD^PdL4G14

Tn10\tetR^rtTA.Act5C

long lived | male limited, with Hsap\UBB^tetO.cHa, Scer\GAL4^da.G32

Tn10\tetR^{rtTA(M2-alt).UAS}

long lived | RU486 conditional, with PdL73A1^PdL73A1

Tn10\tetR^rtTA.Act5C

long lived | RU486 conditional, with α-Man-IIa^PdL19B3

Tn10\tetR^rtTA.Act5C

melanotic mass phenotype | P-stage, with Hsap\UBB^tetO.cHa

Tn10\tetR^rtTA.Act5C

short lived, with MRG15^RNAi.tetO

Tn10\tetR^rtTA.Act5C

short lived, with PdL14B1^PdL14B1

Tn10\tetR^rtTA.Act5C

short lived | :heat sensitive | tetracycline conditional, with Hsp22^tetO.cBa

Tn10\tetR^rtTA.Act5C

short lived | heat sensitive, with Sod2^tetO.cCa

Tn10\tetR^rtTA.Act5C

short lived | male limited, with Hsap\UBB^+1.tetO, Scer\GAL4^da.G32

Tn10\tetR^{rtTA(M2-alt).UAS}

short lived | RU486 conditional, with ash1^PdL11A3

Tn10\tetR^rtTA.Act5C

short lived | RU486 conditional, with PdL2B1^PdL2B1

Tn10\tetR^rtTA.Act5C

short lived | RU486 conditional, with PdL14B1^PdL14B1

Tn10\tetR^rtTA.Act5C

short lived | RU486 conditional, with PdL35B1^PdL35B1

Tn10\tetR^rtTA.Act5C

short lived | RU486 conditional, with PdL43C1^PdL43C1

Tn10\tetR^rtTA.Act5C

short lived | RU486 conditional, with pum^PdL58A2

Tn10\tetR^rtTA.Act5C

short lived | tetracycline conditional, with Sod2^tetO.cFa

Tn10\tetR^rtTA.Act5C

some die during P-stage, with Hsap\UBB^tetO.cHa

Tn10\tetR^rtTA.Act5C

visible, with pum^PdL3B2

Tn10\tetR^rtTA.Act5C

visible, with pum^PdL3G

Tn10\tetR^rtTA.Act5C

visible | adult stage | RU486 conditional, with α-Man-IIa^PdL19B3

Tn10\tetR^rtTA.Act5C

visible | RU486 conditional, with PdL35B1^PdL35B1

Tn10\tetR^rtTA.Act5C

visible | RU486 conditional, with pum^PdL58A2

Tn10\tetR^rtTA.Act5C

visible | tetracycline conditional, with Scer\GAL4^salm-459.2, rpr^tetO.cSa

Tn10\tetR^{rtTA(M2-alt).UAS}

wild-type, with Scr^{DD.tetO.Tag:HA}

Tn10\tetR^tTA.hs

Phenotype manifest in

Allele

adult abdomen, with CaMKII^T287D.tetO, Scer\GAL4^29BD

Tn10\tetR^tTA.UAS

dorsal appendage | drug conditional, with dbr^3M1753

Tn10\tetR^rtTA.Act5C

dorsal appendage | drug conditional, with edl^4M127

Tn10\tetR^rtTA.Act5C

dorsal appendage | drug conditional, with foxo^Y2-26

Tn10\tetR^rtTA.Act5C

dorsal appendage | drug conditional, with wmd^pter-2-4

Tn10\tetR^rtTA.Act5C

egg chorion | drug conditional, with foxo^Y2-26

Tn10\tetR^rtTA.Act5C

egg chorion | drug conditional, with ush^3-38

Tn10\tetR^rtTA.Act5C

embryo | dorsal closure stage, with Scr^{AA.tetO.Tag:HA}

Tn10\tetR^tTA.hs

embryo | dorsal closure stage, with Scr^tetO.Tag:HA

Tn10\tetR^tTA.hs

embryonic head segment | dorsal, with Antp^tetO.cBa

Tn10\tetR^tTA.hs

eye, with Antp^tetO.cBa

Tn10\tetR^tTA.ey.3.5

eye | RU486 conditional, with α-Man-IIa^PdL19B3

Tn10\tetR^rtTA.Act5C

eye | tetracycline conditional, with Scer\GAL4^salm-459.2, rpr^tetO.cSa

Tn10\tetR^{rtTA(M2-alt).UAS}

larval head, with Scr^{AA.tetO.Tag:HA}

Tn10\tetR^tTA.hs

larval head, with Scr^tetO.Tag:HA

Tn10\tetR^tTA.hs

micropyle | drug conditional, with dbr^3M1753

Tn10\tetR^rtTA.Act5C

prothoracic ventral denticle belt | embryonic stage, with Antp^tetO.cBa

Tn10\tetR^tTA.hs

pupa, with Hsap\UBB^tetO.cHa

Tn10\tetR^rtTA.Act5C

salivary gland | ectopic, with Scr^{AA.tetO.Tag:HA}

Tn10\tetR^tTA.hs

salivary gland | ectopic, with Scr^tetO.Tag:HA

Tn10\tetR^tTA.hs

wing, with CaMKII^T287D.tetO, Scer\GAL4^29BD

Tn10\tetR^tTA.UAS

wing, with pum^PdL3B2

Tn10\tetR^rtTA.Act5C

wing, with pum^PdL3G

Tn10\tetR^rtTA.Act5C

wing | RU486 conditional, with PdL35B1^PdL35B1

Tn10\tetR^rtTA.Act5C

wing | RU486 conditional, with pum^PdL58A2

Tn10\tetR^rtTA.Act5C

wing | tetracycline conditional, with Scer\GAL4^salm-459.2, rpr^tetO.cSa

Tn10\tetR^{rtTA(M2-alt).UAS}

Orthologs

Downloads

Download All DIOPT Orthologs

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Drosophila melanogaster (Fruit fly) (0)

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

Class of Gene

engineered_foreign_gene

Stocks and Reagents

Stocks (6)

26801

P{ey-tTA}3, ry⁵⁰⁶

91381

w^*; M{fln-tTA.G}ZH-86Fa

27891

y¹ w^*; P{UAS-tTA.S}4

26802

y¹ w^*; P{Hsp70.tTA.Ba}2

26803

y¹ w^*; P{Hsp70.tTA.Ba}3

92005

w¹¹¹⁸; P{UAS-rtTA-M2-alt}901, P{tetO-PTX.C}20f/CyO

Other Comments

A component of the tetracyclin (tet)-regulated transactivation system: Tn10\tetR-regulated transactivating protein under the control of regulatory sequences is crossed to a strain containing the coding sequence for a gene of interest downstream of the Tn10\tetO operator sequences. Expression of the gene can be tightly controlled by Tn10\tetR. This system is used to study SAR function in vivo, a synthetic SAR-binding protein is overexpressed by means of the Tet system.

(Girard et al., 1998)

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

TetR

(Hoe et al., 2011, Ryu et al., 2001, Nilsson et al., 2000)

Tn10\tetR

pSC101\tetR

rtTA

(Haussmann et al., 2013, Li and Tower, 2009, Curtis et al., 2007, Khokhar et al., 2004, Landis et al., 2001)

tTA

(Mehren and Griffith, 2004, Horn et al., 2003, Horn et al., 2002, Stebbins and Yin, 2001, Bello et al., 1998)

tTa

(Thomas et al., 2000)

tetR

(Schuh et al., 2007, Schuh et al., 2007, Alphey, 2002)

Name Synonyms

tetracyclin repressor

Secondary FlyBase IDs

FBgn0024597

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 0 )

References (48)