FlyBase Allele Report: Dmel\Zfrp8[GL00541]

General Information

Symbol

Dmel\Zfrp8^GL00541

Species

D. melanogaster

Name

FlyBase ID

FBal0266241

Feature type

allele

Associated gene

Dmel\Zfrp8

Associated Insertion(s)

Carried in Construct

P{TRiP.GL00541}

Also Known As

GL00541

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2011.7.19)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2011.7.19)

Mutagen

in vitro construct

(Ni et al., 2011.7.19)

Progenitor genotype

Carried in construct

P{TRiP.GL00541}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2011.7.19)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-GL00541

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

female germline stem cell, with Scer\GAL4^{VP16.nanos.UTR}

(Minakhina et al., 2014)

ovariole, with Scer\GAL4^{VP16.nanos.UTR}

(Minakhina et al., 2014)

ovary | adult stage, with Scer\GAL4^nanos.PU

(Minakhina et al., 2016)

spectrosome, with Scer\GAL4^{VP16.nanos.UTR}

(Minakhina et al., 2014)

Detailed Description

Statement

Reference

Expression of Zfrp8^GL00541 under the control of Scer\GAL4^nos.PU results in morphological defects in the ovary.

(Minakhina et al., 2016)

Expression of Zfrp8^GL00541 under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} results in ovarian defects that increase in severity with age. The organisation of the germarium is almost normal in females less than 2 days old, and contains germline stem cells that give rise to a few egg chambers. Ovarioles of 7-10 day old females either contain a large cyst of cells instead of an organised germarium and egg chambers or contain ovarioles that have lost most or all of the germline cells. The spectrosomes of the mutant germline stem cells are often replaced by a fusome-like structure.

(Minakhina et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^nanos.PU, Zfrp8^GL00541 has ovary | adult stage phenotype, enhanceable by RpS2[+]/RpS2^P

(Minakhina et al., 2016)

Scer\GAL4^nanos.PU, Zfrp8^GL00541 has ovary | adult stage phenotype, enhanceable by RpS2[+]/RpS2^PRW1

(Minakhina et al., 2016)

Suppressed by

Statement

Reference

Scer\GAL4^{VP16.nanos.UTR}, Zfrp8^GL00541 has ovary phenotype, suppressible by Hsap\PDCD2^UASp.EGFP, Scer\GAL4^{VP16.nanos.UTR}

(Minakhina et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

RpS2^P/+ or RpS2^PRW1/+ enhances the ovary defects of flies expressing Zfrp8^GL00541 under the control of Scer\GAL4^nos.PU.

(Minakhina et al., 2016)

Xenogenetic Interactions

Statement

Reference

Co-expression of Hsap\PDCD2^{Scer\UAS.P\T.T:Avic\GFP-EGFP} suppresses the ovarian defects caused by expression of Zfrp8^GL00541 under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}.

(Minakhina et al., 2014)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

36581

y¹ sc^* v¹ sev²¹; P{TRiP.GL00541}attP2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Zfrp8^GL00541

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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