FlyBase Allele Report: Dmel\Nop60B[GL00555]

General Information

Symbol

Dmel\Nop60B^GL00555

Species

D. melanogaster

Name

FlyBase ID

FBal0266255

Feature type

allele

Associated gene

Dmel\Nop60B

Associated Insertion(s)

Carried in Construct

P{TRiP.GL00555}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2011.7.19)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2011.7.19)

Mutagen

in vitro construct

(Ni et al., 2011.7.19)

Progenitor genotype

Carried in construct

P{TRiP.GL00555}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2011.7.19)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-GL00555

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of dyskeratosis congenita

CEA

(Vicidomini et al., 2015)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased fecundity, with Scer\GAL4^nanos.PG

(Breznak et al., 2023)

female sterile, with Scer\GAL4^nanos.PG

(Breznak et al., 2023)

increased cell death | third instar larval stage, with Scer\GAL4^bi.PU

(Vicidomini et al., 2015)

increased cell number | third instar larval stage, with Scer\GAL4^bi.PU

(Vicidomini et al., 2015)

Phenotype Manifest In

Detailed Description

Statement

Reference

Third instar larvae (at 96 and 120h after egg deposition) with expression of Nop60B^GL00555 driven by Scer\GAL4^bi.PU show strong tissue disorganization and increased cell death along the A/P border in wing discs; there is evidence for apoptosis-induced proliferation (significant increase in proliferating cells). Epithelial-mesenchymal transition is induced in the peripodial membrane (cell fate changes) with silencing of Nop60B^GL00555 driven by Scer\GAL4^bi.PU in third instar larval wing discs.

(Vicidomini et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Nop60B^GL00555 has increased cell number | third instar larval stage phenotype, enhanceable by BacA\p35^UAS.cHa/Scer\GAL4^bi.PU

(Vicidomini et al., 2015)

Nop60B^GL00555, Scer\GAL4^bi.PU has increased cell death | third instar larval stage phenotype, enhanceable by BacA\p35^UAS.cHa, Scer\GAL4^bi.PU

(Vicidomini et al., 2015)

Suppressed by

Statement

Reference

Nop60B^GL00555, Scer\GAL4^bi.PU has increased cell death | third instar larval stage phenotype, suppressible by wg^SH1281

(Vicidomini et al., 2015)

Phenotype Manifest In

Enhanced by

Statement

Reference

Nop60B^GL00555 has wing disc | third instar larval stage phenotype, enhanceable by BacA\p35^UAS.cHa/Scer\GAL4^bi.PU

(Vicidomini et al., 2015)

Suppressed by

Statement

Reference

Nop60B^GL00555, Scer\GAL4^nanos.PG has egg chamber | absent phenotype, suppressible | partially by raptor^UAS.Tag:HA, Scer\GAL4^nanos.PG

(Breznak et al., 2023)

Nop60B^GL00555, Scer\GAL4^nanos.PG has germarium phenotype, suppressible | partially by raptor^UAS.Tag:HA, Scer\GAL4^nanos.PG

(Breznak et al., 2023)

Nop60B^GL00555, Scer\GAL4^bi.PU has wing disc | third instar larval stage phenotype, suppressible by wg^SH1281

(Vicidomini et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

wg^SH1281 suppresses apoptosis-induced increases in proliferative activity in Scer\GAL4^bi.PU>Nop60B^GL00555 wing discs.

(Vicidomini et al., 2015)

Xenogenetic Interactions

Statement

Reference

Co-expression of BacA\p35^Scer\UAS.cHa enhances wing disc phenotypes, with 'undead' cells secreting high levels of wg along the A/P margin, in Scer\GAL4^bi.PU>Nop60B^GL00555 third instar larval wing discs; co-expression of BacA\p35^Scer\UAS.cHa also further increases mitotic activity.

(Vicidomini et al., 2015)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

36595

y¹ v¹; P{TRiP.GL00555}attP2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Nop60B^GL00555

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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