FlyBase Allele Report: Dmel\Polr2A[GL01068]

General Information

Symbol

Dmel\Polr2A^GL01068

Species

D. melanogaster

Name

FlyBase ID

FBal0266385

Feature type

allele

Associated gene

Dmel\Polr2A

Associated Insertion(s)

Carried in Construct

P{TRiP.GL01068}

Also Known As

UAS-Rpb1i

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2011.7.19)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2011.7.19)

Mutagen

in vitro construct

(Ni et al., 2011.7.19)

Progenitor genotype

Carried in construct

P{TRiP.GL01068}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2011.7.19)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-GL01068

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

wing, with Scer\GAL4^Bx-MS1096

(Lu et al., 2019)

Detailed Description

Statement

Reference

Expressing RpII215^GL01068 under the control of Scer\GAL4^Bx-MS1096 induces severely distorted, miniature-sized wings.

(Lu et al., 2019)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Polr2A^GL01068 is rescued by Polr2A^{20con.UAS.Tag:FLAG}/Scer\GAL4^Act5C.PI

(Lu et al., 2019)

Polr2A^GL01068 is rescued by Polr2A^{20con.UAS.Tag:FLAG}/Scer\GAL4^Bx-MS1096

(Lu et al., 2019)

Polr2A^GL01068 is rescued by Polr2A^{24con.UAS.Tag:FLAG}/Scer\GAL4^Act5C.PI

(Lu et al., 2019)

Polr2A^GL01068 is rescued by Polr2A^{24con.UAS.Tag:FLAG}/Scer\GAL4^Bx-MS1096

(Lu et al., 2019)

Polr2A^GL01068 is rescued by Polr2A^{29con.UAS.Tag:FLAG}/Scer\GAL4^Bx-MS1096

(Lu et al., 2019)

Polr2A^GL01068 is rescued by Polr2A^{29con.UAS.Tag:FLAG}/Scer\GAL4^Act5C.PI

(Lu et al., 2019)

Polr2A^GL01068 is rescued by Polr2A^{WT.res.UAS.Tag:FLAG}/Scer\GAL4^Act5C.PI

(Lu et al., 2019)

Polr2A^GL01068 is rescued by Polr2A^{WT.res.UAS.Tag:FLAG}/Scer\GAL4^Bx-MS1096

(Lu et al., 2019)

Polr2A^GL01068 is rescued by Scer\GAL4^Act5C.PI/Polr2A^{UAS.RR.Tag:FLAG}

(Gibbs et al., 2017)

Polr2A^GL01068 is rescued by Scer\GAL4^Act5C.PI/Polr2A^{CTDΔ4.UAS.RR.Tag:FLAG}

(Gibbs et al., 2017)

Partially rescued by

Polr2A^GL01068 is partially rescued by Scer\GAL4^Act5C.PI/Polr2A^{42con.UAS.Tag:FLAG}

(Lu et al., 2019)

Polr2A^GL01068 is partially rescued by Scer\GAL4^Bx-MS1096/Polr2A^{42con.UAS.Tag:FLAG}

(Lu et al., 2019)

Polr2A^GL01068 is partially rescued by Polr2A^{52con.UAS.Tag:FLAG}/Scer\GAL4^Bx-MS1096

(Lu et al., 2019)

Polr2A^GL01068 is partially rescued by Scer\GAL4^Act5C.PI/Polr2A^{human.UAS.Tag:FLAG}

(Lu et al., 2019)

Polr2A^GL01068 is partially rescued by Polr2A^{human.UAS.Tag:FLAG}/Scer\GAL4^Bx-MS1096

(Lu et al., 2019)

Polr2A^GL01068 is partially rescued by Polr2A^{CTDΔ1.UAS.RR.Tag:FLAG}/Scer\GAL4^Act5C.PI

(Gibbs et al., 2017)

Polr2A^GL01068 is partially rescued by Scer\GAL4^Act5C.PI/Polr2A^{CTDΔ3.UAS.RR.Tag:FLAG}

(Gibbs et al., 2017)

Not rescued by

Polr2A^GL01068 is not rescued by Scer\GAL4^Act5C.PI/Polr2A^{10con.UAS.Tag:FLAG}

(Lu et al., 2019)

Polr2A^GL01068 is not rescued by Polr2A^{10con.UAS.Tag:FLAG}/Scer\GAL4^Bx-MS1096

(Lu et al., 2019)

Polr2A^GL01068 is not rescued by Polr2A^{52con.UAS.Tag:FLAG}/Scer\GAL4^Act5C.PI

(Lu et al., 2019)

Polr2A^GL01068 is not rescued by Scer\GAL4^Act5C.PI/Polr2A^{CTDΔ2.UAS.RR.Tag:FLAG}

(Gibbs et al., 2017)

Comments

The lethality of flies expressing RpII215^GL01068 RNAi under the control of Scer\GAL4^Act5C.PI is rescued (to a varying degree) by co-expression of any of the following: RpII215^{Scer\UAS.RR.T:Zzzz\FLAG}, RpII215^{CTDΔ1.Scer\UAS.RR.T:Zzzz\FLAG}, RpII215^{CTDΔ3.Scer\UAS.RR.T:Zzzz\FLAG} or RpII215^{CTDΔ4.Scer\UAS.RR.T:Zzzz\FLAG} but no viable progeny is recovered upon co-expression of RpII215^{CTDΔ2.Scer\UAS.RR.T:Zzzz\FLAG}.

(Gibbs et al., 2017)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

36830

y¹ v¹; P{TRiP.GL01068}attP2/TM3, Sb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Polr2A^GL01068

RpII215^GL01068

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections