UASt regulatory sequences drive expression of RNAi-resistant (to RpII215GL01068) RpII215 with a deletion in the carboxy-terminal domain (CTD; 1579-1678), tagged at the C-terminus with 2xTag:FLAG.
Polr2ACTDΔ1.UAS.RR.Tag:FLAG/Scer\GAL4Act5C.PI partially rescues Polr2AGL01068
The lethality of flies expressing RpII215GL01068 RNAi under the control of Scer\GAL4Act5C.PI is rescued by co-expression of RpII215CTDΔ1.Scer\UAS.RR.T:Zzzz\FLAG.