FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Sema2bGFP,Tag:M(mCd8a)
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General Information
Symbol
Dmel\Sema2bGFP,Tag:M(mCd8a)
Species
D. melanogaster
Name
FlyBase ID
FBal0266713
Feature type
allele
Associated gene
Dmel\Sema2b
Associated Insertion(s)
Carried in Construct
PBac{Sema2b.TM}
Key Links
Transgenic product class
wild_type
(Wu et al., 2011)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Wu et al., 2011)
Mutagen
in vitro construct
(Wu et al., 2011)
Progenitor genotype
Sema2bTag:HA
(Wu et al., 2011)
Carried in construct
PBac{Sema2b.TM}
Cytology
Description

Sequences encoding the transmembrane region of Mmus\Cd8a and the GFP coding region have been introduced into the Sema2b coding sequence.

(Wu et al., 2011)
Allele components
Component
Use(s)
Regulatory region(s)
Sema2b
Encoded product / tool
Sema2b
Tagged with
GFP
Tag:M(mCd8a)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Sema-2bT:Avic\GFP,T:Mmus\Cd8a under the control of Scer\GAL4peb-GAL4 restores the dorsolateral/ventromedial axon bundle ratio in Sema-2bf02042 mutants. However, expression at 29[o]C results in differential Sema-2bT:Avic\GFP,T:Mmus\Cd8a expression between the two bundles, with a bias towards the ventromedial trajectory, in some cases almost completely removing the dorsolateral bundle. Sufficiently high levels of Sema-2b can thus force dorsolateral axons to choose the ventromedial trajectory.

      (Joo et al., 2013)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Sema2bGFP,Tag:M(mCd8a)/Scer\GAL4peb-GAL4 rescues Sema2bf02042

      (Joo et al., 2013)

      Sema2bGFP,Tag:M(mCd8a) rescues Df(2R)Sema2b-C4

      (Wu et al., 2011)
      Comments

      Sema-2b+tWa almost completely rescues the longitudinal connective defects of Df(2R)Sema-2b-C4 mutant embryos, including the defects seen in the intermediate Fas2-positive longitudinal tracts.

      (Wu et al., 2011)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Sema-2bT:Avic\GFP,T:Mmus\Cd8a
      Sema2bGFP,Tag:M(mCd8a)
      Sema2bT:Avic\GFP,T:Mmus\Cd8a
      Name Synonyms
      Secondary FlyBase IDs
        References (3)