An intronless Myb cDNA in which the TPT amino acid sequence starting at position 445 has been replaced with AAA is expressed under control of the native Myb promoter. The construct is tagged with EGFP.
MybTPT445.EGFP/Scer\GAL4da.G32 fails to rescue MybMH107
Expression of MybTPT445.T:Avic\GFP-EGFP is unable to rescue MybMH107 mutant lethality at either 25[o]C or 29[o]C. Weak rescue is seen at 18[o]C.