FlyBase Allele Report: Dmel\Myb[MH107]

General Information

Symbol

Dmel\Myb^MH107

Species

D. melanogaster

Name

FlyBase ID

FBal0141652

Feature type

allele

Associated gene

Dmel\Myb

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

Mutagen

Delta2-3 transposase

(Manak et al., 2002)

Progenitor genotype

P{EP}Gβ13F^EP1071

(Manak et al., 2002)

Caused by aberration

Df(1)MH107

Cytology

Description

Deletion removing the promoter and 5' untranslated region of Myb (and the adjacent AlkB gene).

(Beall et al., 2002)

Imprecise excision of P{EP}Gβ13F^EP1071 resulted in deletion of a 3' portion of the original P element along with adjacent DNA including the entire AlkB gene as well the 5' untranslated region of Myb.

(Manak et al., 2002)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

centrosome

(Manak et al., 2002)

chromosome

(Wen et al., 2008)

condensed nuclear chromosome

(Manak et al., 2007)

dorsal appendage | somatic clone

(Beall et al., 2002)

egg chorion | somatic clone

(Beall et al., 2002)

embryonic/larval crystal cell

(Davidson et al., 2005)

embryonic/larval lymph gland

(Davidson et al., 2005)

embryonic/larval plasmatocyte

(Davidson et al., 2005)

G2 phase

(Manak et al., 2002)

hemocyte & nucleus

(Davidson et al., 2005)

mitosis & nuclear chromosome

(Manak et al., 2002)

mitotic anaphase

(Manak et al., 2002)

mitotic cell cycle

(Manak et al., 2002)

mitotic M phase

(Manak et al., 2002)

neuroblast

(Wen et al., 2008)

S phase

(Manak et al., 2002)

spindle

(Wen et al., 2008, Manak et al., 2002)

Detailed Description

Statement

Reference

Embryos derived from females carrying homozygous Myb^MH107 germline clones crossed to wild type males are able to hatch at a similar rate to wild type.

(Lewis et al., 2012)

Myb^MH107 mutant neuroblasts display characteristics of chromosome condensation and are dramatically delayed in prometaphase/metaphase with persistently abnormal spindles. The initially bipolar spindle poles migrate abnormally, and the spindle itself is often rotated, bowed and/or mis-attached.

(Wen et al., 2008)

Mosaic analysis in third instar larval imaginal eye discs of Myb^MH107 mutant clones and adjacent twin spots does not reveal any abnormalities associated with the mutant cells regarding cell size and cell number as well as cell doubling time. In Myb^MH107 mutant clones in larval brain, neither S phase nor M phase are altered, nor is there an unusual overlap of DNA synthesis and chromosome condensation. Myb^MH107 mutant cells contribute to normal appearing clones in pupal and adult eyes. Eyes composed of entirely of Myb^MH107 mutant cells appear normal.

Examination of Myb^MH107 mutant eye and wing imaginal discs reveals an increased frequency of cells with condensed chromosomes compared to controls. In Myb^MH107 mutant wing imaginal discs, small clustered masses of partially condensed chromatin are observed. Many individual nuclei contain several condensed masses adjacent to and within a single nuclear envelope, consistent with arrest and /or delay in early prophase.

The apoptotic response to ionizing radiation appears normal in Myb^MH107 mutant wing imaginal disc cells.

(Manak et al., 2007)

Homozygous larvae are completely devoid of crystal cells and plasmatocyte numbers are reduced. The lymph glands of homozygous third instar larvae are undersized compared to those of control animals. The reduced number of hemocytes in the lymph gland have aberrant nuclei. Somatic clones of homozygous hemocytes can be recovered and are seen both in the lymph gland and in the hemolymph. These hemocytes show a phagocytosis defect, showing a 57-68% decrease in phagocytosis compared to control cells.

(Davidson et al., 2005)

Homozygous clones in stage 10 egg chambers do not contain subnuclear foci of BrdU incorporation in contrast to surrounding normal cells. Homozygous Myb^MH107 clones (Df(1)MH107 clones in which AlkB function has been rescued by AlkB^+t9.8) in the region of the egg chamber normally responsible for dorsal appendage formation result in greatly reduced dorsal appendages. The chorion is missing under the mutant follicle cell patch.

(Beall et al., 2002)

Late third instar Myb^MH107 mutant wing discs have a lower proportion of cells with G2 DNA content than wild-type. These mutant wing discs also exhibit a 2-3 fold increase in the number of cells with condensed chromosomes compared to wild-type. In many cases the chromosomes in mutant cells become "hyper-condensed". Some mutant cells exhibit overlap between M-phase and S phase: chromsome condensation and DNA replication can be detected in the same cells. There is also a substantial increase in apoptosis in Myb^MH107 mutant discs. Myb^MH107 mutant cells in the third instar larval brain with condensed chromosomes often contain one or no centrosomes. Centromeres are formed in these mutant cells, but they exhibit a variety of abnormalities, including monopolar spindle attachement, multipolar spindles and abnormalities in ploidy: about 5% of all mutant mitoses are aneuploid and 1-2% are polyploid. There is also a decrease in the proportion of cells in anaphase in Myb^MH107 mutant third instar larval brains to 4%, compared with 9% in wild-type.

(Manak et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Myb^MH107, lin-52⁹³ has visible | somatic clone | adult stage phenotype, suppressible by mip130^1-36

(Lewis et al., 2012)

Myb^MH107 has lethal | recessive phenotype, suppressible by mip130^1-36

(Beall et al., 2004)

Myb^MH107 has lethal | recessive phenotype, suppressible by mip130^1-723+1-36

(Beall et al., 2004)

NOT Enhancer of

Statement

Reference

Myb^MH107 is a non-enhancer of female semi-sterile phenotype of mip130^1-36

(Beall et al., 2004)

Suppressor of

Statement

Reference

Myb^MH107 is a suppressor of abnormal size | third instar larval stage phenotype of Tl^10b

(Davidson et al., 2005)

Myb^MH107 is a suppressor of increased cell number | larval stage phenotype of Tl^10b

(Davidson et al., 2005)

Myb^MH107 is a suppressor of increased cell number | larval stage phenotype of hop^Tum

(Davidson et al., 2005)

NOT Suppressor of

Statement

Reference

Myb^MH107 is a non-suppressor of visible | somatic clone | adult stage phenotype of lin-52⁹³

(Lewis et al., 2012)

(Lewis et al., 2012)

Myb^MH107 is a non-suppressor of female semi-sterile phenotype of mip130^1-36

(Beall et al., 2004)

Myb^MH107 is a non-suppressor of female sterile phenotype of mip130^1-723+1-36

(Beall et al., 2004)

Phenotype Manifest In

Suppressed by

Statement

Reference

Myb^MH107, lin-52⁹³ has eye | somatic clone phenotype, suppressible by mip130^1-36

(Lewis et al., 2012)

Myb^MH107, lin-52⁹³ has ommatidium | somatic clone | adult stage phenotype, suppressible by mip130^1-36

(Lewis et al., 2012)

Myb^MH107, lin-52⁹³ has rhabdomere | somatic clone | adult stage phenotype, suppressible by mip130^1-36

(Lewis et al., 2012)

Myb^MH107 has hemocyte & nucleus phenotype, suppressible by Scer\GAL4^e33C/Ggal\B-Myb^UAS.cDa

(Davidson et al., 2005)

Myb^MH107 has embryonic/larval lymph gland phenotype, suppressible by Scer\GAL4^e33C/Ggal\B-Myb^UAS.cDa

(Davidson et al., 2005)

Myb^MH107 has embryonic/larval crystal cell phenotype, suppressible by Scer\GAL4^e33C/Ggal\B-Myb^UAS.cDa

(Davidson et al., 2005)

NOT suppressed by

Statement

Reference

Myb^MH107 has embryonic/larval crystal cell phenotype, non-suppressible by Scer\GAL4^e33C/Ggal\A-Myb^UAS.cDa

(Davidson et al., 2005)

Myb^MH107 has embryonic/larval crystal cell phenotype, non-suppressible by Scer\GAL4^e33C/Ggal\c-Myb^UAS.cDa

(Davidson et al., 2005)

NOT Suppressor of

Statement

Reference

Myb^MH107 is a non-suppressor of eye | somatic clone phenotype of lin-52⁹³

(Lewis et al., 2012)

Myb^MH107 is a non-suppressor of rhabdomere | somatic clone | adult stage phenotype of lin-52⁹³

(Lewis et al., 2012)

Myb^MH107 is a non-suppressor of ommatidium | somatic clone | adult stage phenotype of lin-52⁹³

(Lewis et al., 2012)

Myb^MH107 is a non-suppressor | germline clone | maternal effect of embryo | germline clone | maternal effect phenotype of lin-52⁹³

(Lewis et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Homozygous Myb^MH107 does not suppress the eye phenotypes seen in lin-52⁹³ mutant eye clones.

Homozygous mip130^1-36 suppresses the eye phenotypes seen in Myb^MH107, lin-52⁹³ double mutant clones. The resulting triple mutant eyes appear completely normal.

Approximately 2% of embryos derived from females carrying homozygous lin-52⁹³ Myb^MH107 double mutant germline clones crossed to wild type males are able to hatch, a similar rate to when females are maternally mutant for lin-52⁹³ alone.

(Lewis et al., 2012)

Hemocytes in the secondary lymph gland lobes of hop^Tum Myb^MH107 double mutant larvae fail to overproliferate and do not adopt the flattened shape characteristic of differentiated lamellocytes. Abnormal differentiation of lamellocytes is not seen in the secondary lymph gland lobes of Tl⁸ Myb^MH107 double mutant larvae. The lymph gland hemocytes fail to overproliferate in the double mutant larvae.

(Davidson et al., 2005)

Xenogenetic Interactions

Statement

Reference

Expression of Ggal\B-Myb^Scer\UAS.cDa under the control of Scer\GAL4^e33C restores circulating crystal cells and crystal cells in the lymph gland in homozygous Myb^MH107 larvae. The mutant phenotype seen in the Myb^MH107 lymph glands are also rescued by expression of Ggal\B-Myb^Scer\UAS.cDa under the control of Scer\GAL4^e33C. Expression of Ggal\c-Myb^Scer\UAS.cDa or Ggal\A-Myb^Scer\UAS.cDa under the control of Scer\GAL4^e33C does not rescue circulating crystal cells in Myb^MH107 larvae.

(Davidson et al., 2005)

Complementation and Rescue Data

Rescued by

Myb^MH107 is rescued by Myb^{K172.UAS.EGFP}/Scer\GAL4^da.G32

(Andrejka et al., 2011)

Myb^MH107 is rescued by Scer\GAL4^da.G32/Myb^{N180.UAS.EGFP}

(Andrejka et al., 2011)

Myb^MH107 is rescued by Scer\GAL4^da.G32/Myb^UAS.N.EGFP

(Andrejka et al., 2011)

Myb^MH107 is rescued by Myb^EGFP

(Andrejka et al., 2011)

Myb^MH107 is rescued by Scer\GAL4^da.G32/Myb^DKD36.EGFP

(Andrejka et al., 2011)

Myb^MH107 is rescued by Scer\GAL4^da.G32/Myb^PP374.EGFP

(Andrejka et al., 2011)

Myb^MH107 is rescued by Scer\GAL4^da.G32/Myb^FLK393.EGFP

(Andrejka et al., 2011)

Myb^MH107 is rescued by Myb^{UAS.N.mRFP(Unk)}

(Wen et al., 2008)

Myb^MH107 is rescued by Myb^UAS.N.EGFP

(Wen et al., 2008)

Myb^MH107 is rescued by Myb^{Cterm.UAS.EGFP}

(Wen et al., 2008)

Myb^MH107 is rescued by Scer\GAL4^en-e16E/Myb^UAS.cMa

(Manak et al., 2007)

Myb^MH107 is rescued by Scer\GAL4^e33C/Myb^UAS.cDa

(Davidson et al., 2005)

Partially rescued by

Myb^MH107 is partially rescued by Scer\GAL4^da.G32/Myb^{DKD36.UAS.EGFP}

(Andrejka et al., 2011)

Myb^MH107 is partially rescued by Myb^{ED94.UAS.EGFP}/Scer\GAL4^da.G32

(Andrejka et al., 2011)

Myb^MH107 is partially rescued by Scer\GAL4^da.G32/Myb^{C174.UAS.EGFP}

(Andrejka et al., 2011)

Myb^MH107 is partially rescued by Myb^{E193.UAS.EGFP}/Scer\GAL4^da.G32

(Andrejka et al., 2011)

Myb^MH107 is partially rescued by Scer\GAL4^da.G32/Myb^{DE196.UAS.EGFP}

(Andrejka et al., 2011)

Myb^MH107 is partially rescued by Scer\GAL4^da.G32/Myb^{PP374.UAS.EGFP}

(Andrejka et al., 2011)

Myb^MH107 is partially rescued by Scer\GAL4^da.G32/Myb^{FLK393.UAS.EGFP}

(Andrejka et al., 2011)

Myb^MH107 is partially rescued by Myb^{STP410.UAS.EGFP}/Scer\GAL4^da.G32

(Andrejka et al., 2011)

Myb^MH107 is partially rescued by Myb^{TP435.UAS.EGFP}/Scer\GAL4^da.G32

(Andrejka et al., 2011)

Myb^MH107 is partially rescued by Scer\GAL4^da.G32/Myb^{TPT445.UAS.EGFP}

(Andrejka et al., 2011)

Myb^MH107 is partially rescued by Myb^{EDL475.UAS.EGFP}/Scer\GAL4^da.G32

(Andrejka et al., 2011)

Myb^MH107 is partially rescued by Myb^{RK523.UAS.EGFP}/Scer\GAL4^da.G32

(Andrejka et al., 2011)

Myb^MH107 is partially rescued by Scer\GAL4^da.G32/Myb^{KW606.UAS.EGFP}

(Andrejka et al., 2011)

Myb^MH107 is partially rescued by Scer\GAL4^da.G32/Myb^{DQ617.UAS.EGFP}

(Andrejka et al., 2011)

Myb^MH107 is partially rescued by Myb^{Cterm.UAS.EGFP}/Scer\GAL4^da.G32

(Andrejka et al., 2011)

Myb^MH107 is partially rescued by Myb^KW606.EGFP/Scer\GAL4^da.G32

(Andrejka et al., 2011)

Myb^MH107 is partially rescued by Myb^DQ617.EGFP/Scer\GAL4^da.G32

(Andrejka et al., 2011)

Myb^MH107 is partially rescued by Scer\GAL4^da.G32/Myb^Cterm.EGFP

(Andrejka et al., 2011)

Not rescued by

Myb^MH107 is not rescued by Myb^{Nterm.UAS.EGFP}/Scer\GAL4^da.G32

(Andrejka et al., 2011)

Myb^MH107 is not rescued by Myb^TPT445.EGFP/Scer\GAL4^da.G32

(Andrejka et al., 2011)

Comments

Expression of Myb^{Scer\UAS.N.T:Avic\GFP-EGFP} under the control of Scer\GAL4^da.G32 rescues Myb^MH107 mutant lethality.

Expression of Myb^{Cterm.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^da.G32 rescues Myb^MH107 mutant lethality at 25[o]C, resulting in approximately 50% of the number of adult flies seen in wild type. The rescue is much weaker at 29[o]C.

Expression of Myb^{Nterm.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^da.G32 is unable to rescue Myb^MH107 mutant lethality. An increased number of PH3 positive cells are seen in the wing discs and these cells are delayed or arrested in prophase.

Expression of Myb^{TPT445.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^da.G32 partially rescues Myb^MH107 mutant lethality at 25[o]C, resulting in approximately 15% of the number of adult flies seen in wild type. No rescue is seen at 29[o]C.

Expression of Myb^{KW606.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^da.G32 partially rescues Myb^MH107 mutant lethality at 25[o]C, resulting in approximately 5% of the number of adult flies seen in wild type. No rescue is seen at 29[o]C.

Expression of Myb^{DQ617.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^da.G32 partially rescues Myb^MH107 mutant lethality at 25[o]C, resulting in approximately 10% of the number of adult flies seen in wild type. No rescue is seen at 29[o]C.

Expression of Myb^{DKD36.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^da.G32 partially rescues Myb^MH107 mutant lethality at 25[o]C, resulting in approximately 80% of the number of adult flies seen in wild type. The rescue is much weaker at 29[o]C.

Expression of Myb^{PP374.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^da.G32 partially rescues Myb^MH107 mutant lethality at 25[o]C, resulting in approximately 50% of the number of adult flies seen in wild type. The rescue is much weaker at 29[o]C.

Expression of Myb^{FLK393.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^da.G32 partially rescues Myb^MH107 mutant lethality at 25[o]C, resulting in approximately 30% of the number of adult flies seen in wild type. The rescue is much weaker at 29[o]C.

Expression of Myb^{EDL475.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^da.G32 partially rescues Myb^MH107 mutant lethality at 25[o]C, resulting in approximately 55% of the number of adult flies seen in wild type. The rescue is much weaker at 29[o]C.

Expression of Myb^{RK523.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^da.G32 partially rescues Myb^MH107 mutant lethality at 25[o]C, resulting in approximately 55% of the number of adult flies seen in wild type. The rescue is much weaker at 29[o]C.

Expression of Myb^{T:Avic\GFP-EGFP} fully rescues Myb^MH107 mutant lethality and fertility at 18[o]C, 25[o]C and 29[o]C.

Expression of Myb^{TPT445.T:Avic\GFP-EGFP} is unable to rescue Myb^MH107 mutant lethality at either 25[o]C or 29[o]C. Weak rescue is seen at 18[o]C.

Expression of Myb^{DQ617.T:Avic\GFP-EGFP} is unable to rescue Myb^MH107 mutant lethality at either 25[o]C or 29[o]C. Weak rescue is seen at 18[o]C.

Expression of Myb^{KW606.T:Avic\GFP-EGFP} is unable to rescue Myb^MH107 mutant lethality at either 25[o]C or 29[o]C. Weak rescue is seen at 18[o]C.

Expression of Myb^{Cterm.T:Avic\GFP-EGFP} is unable to rescue Myb^MH107 mutant lethality at either 25[o]C or 29[o]C. Partial rescue is seen at 18[o]C.

Expression of Myb^{DKD36.T:Avic\GFP-EGFP} rescues Myb^MH107 mutant lethality at 25[o]C and 29[o]C.

Expression of Myb^{PP374.T:Avic\GFP-EGFP} rescues Myb^MH107 mutant lethality at 25[o]C and 29[o]C.

Expression of Myb^{FLK393.T:Avic\GFP-EGFP} rescues Myb^MH107 mutant lethality at 25[o]C and 29[o]C.

Expression of Myb^{EDL475.T:Avic\GFP-EGFP} rescues Myb^MH107 mutant lethality at 25[o]C and 29[o]C.

Expression of Myb^{RK523.T:Avic\GFP-EGFP} rescues Myb^MH107 mutant lethality at 25[o]C and 29[o]C.

Expression of Myb^{ED94.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^da.G32 partially rescues Myb^MH107 mutant lethality at 25[o]C, resulting in approximately 60% of the number of adult flies seen in wild type.

Expression of Myb^{K172.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^da.G32 rescues Myb^MH107 mutant lethality at 25[o]C, resulting in a similar number of adult flies as in wild type.

Expression of Myb^{C174.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^da.G32 partially rescues Myb^MH107 mutant lethality at 25[o]C, resulting in approximately 85% of the number of adult flies seen in wild type.

Expression of Myb^{N180.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^da.G32 rescues Myb^MH107 mutant lethality at 25[o]C, resulting in more adult flies than are seen in wild type.

Expression of Myb^{E193.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^da.G32 partially rescues Myb^MH107 mutant lethality at 25[o]C, resulting in approximately 90% of the number of adult flies seen in wild type.

Expression of Myb^{DE196.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^da.G32 partially rescues Myb^MH107 mutant lethality at 25[o]C, resulting in approximately 65% of the number of adult flies seen in wild type.

Expression of Myb^{SPS389.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^da.G32 partially rescues Myb^MH107 mutant lethality at 25[o]C, resulting in approximately 85% of the number of adult flies seen in wild type.

Expression of Myb^{STP410.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^da.G32 partially rescues Myb^MH107 mutant lethality at 25[o]C, resulting in approximately 85% of the number of adult flies seen in wild type.

Expression of Myb^{TP435.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^da.G32 partially rescues Myb^MH107 mutant lethality at 25[o]C, resulting in approximately 25% of the number of adult flies seen in wild type.

(Andrejka et al., 2011)

Expression of Myb^Scer\UAS.cMa driven by Scer\GAL4^en-e16E in the posterior compartment of Myb^MH107 mutant wing discs rescues the condensed chromatin phenotype.

(Manak et al., 2007)

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Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Df(1)MH107

(Andrejka et al., 2011)

MH107

(Beall et al., 2002)

Myb^MH107

(Santana et al., 2020, Debruhl et al., 2013, Andrejka et al., 2011, Manak et al., 2007)

myb^M107

(Lewis et al., 2012)

myb^MH107

(Lewis et al., 2012, Beall et al., 2007)

Name Synonyms

Secondary FlyBase IDs

References (12)

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