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General Information
Symbol
Dmel\MybMH107
Species
D. melanogaster
Name
FlyBase ID
FBal0141652
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Deletion removing the promoter and 5' untranslated region of Myb (and the adjacent AlkB gene).

Imprecise excision of P{EP}Gβ13FEP1071 resulted in deletion of a 3' portion of the original P element along with adjacent DNA including the entire AlkB gene as well the 5' untranslated region of Myb.

Caused by aberration
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Embryos derived from females carrying homozygous MybMH107 germline clones crossed to wild type males are able to hatch at a similar rate to wild type.

MybMH107 mutant neuroblasts display characteristics of chromosome condensation and are dramatically delayed in prometaphase/metaphase with persistently abnormal spindles. The initially bipolar spindle poles migrate abnormally, and the spindle itself is often rotated, bowed and/or mis-attached.

Mosaic analysis in third instar larval imaginal eye discs of MybMH107 mutant clones and adjacent twin spots does not reveal any abnormalities associated with the mutant cells regarding cell size and cell number as well as cell doubling time. In MybMH107 mutant clones in larval brain, neither S phase nor M phase are altered, nor is there an unusual overlap of DNA synthesis and chromosome condensation. MybMH107 mutant cells contribute to normal appearing clones in pupal and adult eyes. Eyes composed of entirely of MybMH107 mutant cells appear normal.

Examination of MybMH107 mutant eye and wing imaginal discs reveals an increased frequency of cells with condensed chromosomes compared to controls. In MybMH107 mutant wing imaginal discs, small clustered masses of partially condensed chromatin are observed. Many individual nuclei contain several condensed masses adjacent to and within a single nuclear envelope, consistent with arrest and /or delay in early prophase.

The apoptotic response to ionizing radiation appears normal in MybMH107 mutant wing imaginal disc cells.

Homozygous larvae are completely devoid of crystal cells and plasmatocyte numbers are reduced. The lymph glands of homozygous third instar larvae are undersized compared to those of control animals. The reduced number of hemocytes in the lymph gland have aberrant nuclei. Somatic clones of homozygous hemocytes can be recovered and are seen both in the lymph gland and in the hemolymph. These hemocytes show a phagocytosis defect, showing a 57-68% decrease in phagocytosis compared to control cells.

Homozygous clones in stage 10 egg chambers do not contain subnuclear foci of BrdU incorporation in contrast to surrounding normal cells. Homozygous MybMH107 clones (Df(1)MH107 clones in which AlkB function has been rescued by AlkB+t9.8) in the region of the egg chamber normally responsible for dorsal appendage formation result in greatly reduced dorsal appendages. The chorion is missing under the mutant follicle cell patch.

Late third instar MybMH107 mutant wing discs have a lower proportion of cells with G2 DNA content than wild-type. These mutant wing discs also exhibit a 2-3 fold increase in the number of cells with condensed chromosomes compared to wild-type. In many cases the chromosomes in mutant cells become "hyper-condensed". Some mutant cells exhibit overlap between M-phase and S phase: chromsome condensation and DNA replication can be detected in the same cells. There is also a substantial increase in apoptosis in MybMH107 mutant discs. MybMH107 mutant cells in the third instar larval brain with condensed chromosomes often contain one or no centrosomes. Centromeres are formed in these mutant cells, but they exhibit a variety of abnormalities, including monopolar spindle attachement, multipolar spindles and abnormalities in ploidy: about 5% of all mutant mitoses are aneuploid and 1-2% are polyploid. There is also a decrease in the proportion of cells in anaphase in MybMH107 mutant third instar larval brains to 4%, compared with 9% in wild-type.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference
NOT Enhancer of
Statement
Reference

MybMH107 is a non-enhancer of female semi-sterile phenotype of mip1301-36

Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference
Phenotype Manifest In
Suppressed by
NOT suppressed by
NOT Suppressor of
Statement
Reference

MybMH107 is a non-suppressor of rhabdomere | somatic clone | adult stage phenotype of lin-5293

MybMH107 is a non-suppressor of ommatidium | somatic clone | adult stage phenotype of lin-5293

MybMH107 is a non-suppressor of eye | somatic clone phenotype of lin-5293

Additional Comments
Genetic Interactions
Statement
Reference

Homozygous MybMH107 does not suppress the eye phenotypes seen in lin-5293 mutant eye clones.

Homozygous mip1301-36 suppresses the eye phenotypes seen in MybMH107, lin-5293 double mutant clones. The resulting triple mutant eyes appear completely normal.

Approximately 2% of embryos derived from females carrying homozygous lin-5293 MybMH107 double mutant germline clones crossed to wild type males are able to hatch, a similar rate to when females are maternally mutant for lin-5293 alone.

Hemocytes in the secondary lymph gland lobes of hopTum MybMH107 double mutant larvae fail to overproliferate and do not adopt the flattened shape characteristic of differentiated lamellocytes. Abnormal differentiation of lamellocytes is not seen in the secondary lymph gland lobes of Tl8 MybMH107 double mutant larvae. The lymph gland hemocytes fail to overproliferate in the double mutant larvae.

Xenogenetic Interactions
Statement
Reference

Expression of Ggal\B-MybScer\UAS.cDa under the control of Scer\GAL4e33C restores circulating crystal cells and crystal cells in the lymph gland in homozygous MybMH107 larvae. The mutant phenotype seen in the MybMH107 lymph glands are also rescued by expression of Ggal\B-MybScer\UAS.cDa under the control of Scer\GAL4e33C. Expression of Ggal\c-MybScer\UAS.cDa or Ggal\A-MybScer\UAS.cDa under the control of Scer\GAL4e33C does not rescue circulating crystal cells in MybMH107 larvae.

Complementation and Rescue Data
Partially rescued by
Comments

Expression of MybScer\UAS.N.T:Avic\GFP-EGFP under the control of Scer\GAL4da.G32 rescues MybMH107 mutant lethality.

Expression of MybCterm.Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4da.G32 rescues MybMH107 mutant lethality at 25[o]C, resulting in approximately 50% of the number of adult flies seen in wild type. The rescue is much weaker at 29[o]C.

Expression of MybNterm.Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4da.G32 is unable to rescue MybMH107 mutant lethality. An increased number of PH3 positive cells are seen in the wing discs and these cells are delayed or arrested in prophase.

Expression of MybTPT445.Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4da.G32 partially rescues MybMH107 mutant lethality at 25[o]C, resulting in approximately 15% of the number of adult flies seen in wild type. No rescue is seen at 29[o]C.

Expression of MybKW606.Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4da.G32 partially rescues MybMH107 mutant lethality at 25[o]C, resulting in approximately 5% of the number of adult flies seen in wild type. No rescue is seen at 29[o]C.

Expression of MybDQ617.Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4da.G32 partially rescues MybMH107 mutant lethality at 25[o]C, resulting in approximately 10% of the number of adult flies seen in wild type. No rescue is seen at 29[o]C.

Expression of MybDKD36.Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4da.G32 partially rescues MybMH107 mutant lethality at 25[o]C, resulting in approximately 80% of the number of adult flies seen in wild type. The rescue is much weaker at 29[o]C.

Expression of MybPP374.Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4da.G32 partially rescues MybMH107 mutant lethality at 25[o]C, resulting in approximately 50% of the number of adult flies seen in wild type. The rescue is much weaker at 29[o]C.

Expression of MybFLK393.Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4da.G32 partially rescues MybMH107 mutant lethality at 25[o]C, resulting in approximately 30% of the number of adult flies seen in wild type. The rescue is much weaker at 29[o]C.

Expression of MybEDL475.Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4da.G32 partially rescues MybMH107 mutant lethality at 25[o]C, resulting in approximately 55% of the number of adult flies seen in wild type. The rescue is much weaker at 29[o]C.

Expression of MybRK523.Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4da.G32 partially rescues MybMH107 mutant lethality at 25[o]C, resulting in approximately 55% of the number of adult flies seen in wild type. The rescue is much weaker at 29[o]C.

Expression of MybT:Avic\GFP-EGFP fully rescues MybMH107 mutant lethality and fertility at 18[o]C, 25[o]C and 29[o]C.

Expression of MybTPT445.T:Avic\GFP-EGFP is unable to rescue MybMH107 mutant lethality at either 25[o]C or 29[o]C. Weak rescue is seen at 18[o]C.

Expression of MybDQ617.T:Avic\GFP-EGFP is unable to rescue MybMH107 mutant lethality at either 25[o]C or 29[o]C. Weak rescue is seen at 18[o]C.

Expression of MybKW606.T:Avic\GFP-EGFP is unable to rescue MybMH107 mutant lethality at either 25[o]C or 29[o]C. Weak rescue is seen at 18[o]C.

Expression of MybCterm.T:Avic\GFP-EGFP is unable to rescue MybMH107 mutant lethality at either 25[o]C or 29[o]C. Partial rescue is seen at 18[o]C.

Expression of MybDKD36.T:Avic\GFP-EGFP rescues MybMH107 mutant lethality at 25[o]C and 29[o]C.

Expression of MybPP374.T:Avic\GFP-EGFP rescues MybMH107 mutant lethality at 25[o]C and 29[o]C.

Expression of MybFLK393.T:Avic\GFP-EGFP rescues MybMH107 mutant lethality at 25[o]C and 29[o]C.

Expression of MybEDL475.T:Avic\GFP-EGFP rescues MybMH107 mutant lethality at 25[o]C and 29[o]C.

Expression of MybRK523.T:Avic\GFP-EGFP rescues MybMH107 mutant lethality at 25[o]C and 29[o]C.

Expression of MybED94.Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4da.G32 partially rescues MybMH107 mutant lethality at 25[o]C, resulting in approximately 60% of the number of adult flies seen in wild type.

Expression of MybK172.Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4da.G32 rescues MybMH107 mutant lethality at 25[o]C, resulting in a similar number of adult flies as in wild type.

Expression of MybC174.Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4da.G32 partially rescues MybMH107 mutant lethality at 25[o]C, resulting in approximately 85% of the number of adult flies seen in wild type.

Expression of MybN180.Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4da.G32 rescues MybMH107 mutant lethality at 25[o]C, resulting in more adult flies than are seen in wild type.

Expression of MybE193.Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4da.G32 partially rescues MybMH107 mutant lethality at 25[o]C, resulting in approximately 90% of the number of adult flies seen in wild type.

Expression of MybDE196.Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4da.G32 partially rescues MybMH107 mutant lethality at 25[o]C, resulting in approximately 65% of the number of adult flies seen in wild type.

Expression of MybSPS389.Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4da.G32 partially rescues MybMH107 mutant lethality at 25[o]C, resulting in approximately 85% of the number of adult flies seen in wild type.

Expression of MybSTP410.Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4da.G32 partially rescues MybMH107 mutant lethality at 25[o]C, resulting in approximately 85% of the number of adult flies seen in wild type.

Expression of MybTP435.Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4da.G32 partially rescues MybMH107 mutant lethality at 25[o]C, resulting in approximately 25% of the number of adult flies seen in wild type.

Expression of MybScer\UAS.cMa driven by Scer\GAL4en-e16E in the posterior compartment of MybMH107 mutant wing discs rescues the condensed chromatin phenotype.

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Synonyms and Secondary IDs (5)
Reported As
Name Synonyms
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    References (12)