FlyBase Allele Report: Hsap\FMR1[UAS.Tag:MYC]

General Information

Symbol

Hsap\FMR1^UAS.Tag:MYC

Species

H. sapiens

Name

FlyBase ID

FBal0267401

Feature type

allele

Associated gene

Hsap\FMR1

Associated Insertion(s)

Carried in Construct

P{UAS-FMR1.MYC}

Also Known As

pUAS-MYC-hFMR1, UAS-hFMR1

Key Links

Transgenic product class

wild_type

(Coffee et al., 2010)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Coffee et al., 2010)

Mutagen

in vitro construct

(Coffee et al., 2012, Coffee et al., 2010)

Progenitor genotype

Carried in construct

P{UAS-FMR1.MYC}

Cytology

Description

UASt drives expression of Hsap\FMR1 tagged with Tag:MYC.

(Coffee et al., 2010)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Hsap\FMR1

Tagged with

Tag:MYC

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^elav.PU/Hsap\FMR1^UAS.Tag:MYC is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Fmr1^Δ50M

(Coffee et al., 2012)

Scer\GAL4^ey-OK107/Hsap\FMR1^UAS.Tag:MYC is a suppressor of abnormal learning | adult stage phenotype of Fmr1^Δ50M

(Coffee et al., 2012)

Scer\GAL4^elav-C155/Hsap\FMR1^UAS.Tag:MYC is a suppressor of abnormal neuroanatomy phenotype of Fmr1^Δ50M

(Coffee et al., 2010)

Scer\GAL4^{VP16.nanos.UTR}/Hsap\FMR1^UAS.Tag:MYC is a suppressor of male sterile phenotype of Fmr1^Δ50M

(Coffee et al., 2010)

Phenotype Manifest In

Suppressor of

Statement

Reference

Scer\GAL4^elav.PU/Hsap\FMR1^UAS.Tag:MYC is a suppressor of embryonic/larval neuromuscular junction | third instar larval stage phenotype of Fmr1^Δ50M

(Coffee et al., 2012)

Scer\GAL4^elav.PU/Hsap\FMR1^UAS.Tag:MYC is a suppressor of NMJ bouton | increased number | third instar larval stage phenotype of Fmr1^Δ50M

(Coffee et al., 2012)

Scer\GAL4^elav.PU/Hsap\FMR1^UAS.Tag:MYC is a suppressor of s-LNv Pdf neuron | increased number | third instar larval stage phenotype of Fmr1^Δ50M

(Coffee et al., 2012)

Scer\GAL4^{VP16.nanos.UTR}/Hsap\FMR1^UAS.Tag:MYC is a suppressor of axonemal microtubule phenotype of Fmr1^Δ50M

(Coffee et al., 2010)

Scer\GAL4^elav-C155/Hsap\FMR1^UAS.Tag:MYC is a suppressor of s-LNv Pdf neuron phenotype of Fmr1^Δ50M

(Coffee et al., 2010)

Scer\GAL4^elav-C155/Hsap\FMR1^UAS.Tag:MYC is a suppressor of bouton phenotype of Fmr1^Δ50M

(Coffee et al., 2010)

Scer\GAL4^elav-C155/Hsap\FMR1^UAS.Tag:MYC is a suppressor of protocerebrum phenotype of Fmr1^Δ50M

(Coffee et al., 2010)

Scer\GAL4^elav-C155/Hsap\FMR1^UAS.Tag:MYC is a suppressor of embryonic/larval neuromuscular junction phenotype of Fmr1^Δ50M

(Coffee et al., 2010)

Scer\GAL4^{VP16.nanos.UTR}/Hsap\FMR1^UAS.Tag:MYC is a suppressor of spermatid axoneme phenotype of Fmr1^Δ50M

(Coffee et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\FMR1^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^elav.PU rescues the neuromuscular junction synapse architecture defects seen in Fmr1^Δ50M mutant third instar larvae. The increase in Futsch-positive loops is also fully rescued.

Expressing Hsap\FMR1^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^elav.PU rescues the expanded terminals and supernumerary synaptic boutons seen in the small ventrolateral neurons of Fmr1^Δ50M mutant adults.

Expression of Hsap\FMR1^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^ey-OK107 rescues the decreases in olfactory learning seen in Fmr1^Δ50M mutants.

(Coffee et al., 2012)

Expression of Hsap\FMR1^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^elav-C155 completely suppresses the synaptic overgrowth defects characterising Fmr1^Δ50M mutants.

Expression of Hsap\FMR1^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^elav-C155 fully rescues both the enlarged neuromuscular junctional area and increased synaptic branching that characterise Fmr1^Δ50M mutants.

Pre-synaptic expression of Hsap\FMR1^{Scer\UAS.T:Hsap\MYC} using Scer\GAL4^elav-C155 completely restores bouton number to control levels in Fmr1^Δ50M mutants.

Scer\GAL4^elav-C155>Hsap\FMR1^{Scer\UAS.T:Hsap\MYC} can strongly rescue the mini-bouton number in Fmr1^Δ50M mutants back to wild-type levels.

Expression of Hsap\FMR1^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} completely suppresses the male fecundity phenotype that characterises Fmr1^Δ50M mutants.

Expression of Hsap\FMR1^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} can fully restore the spermatid axoneme architecture in Fmr1^Δ50M mutants.

(Coffee et al., 2010)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Hsap\FMR1^{Scer\UAS.T:Hsap\MYC}

Hsap\FMR1^UAS.Tag:MYC

Name Synonyms

Secondary FlyBase IDs

References (3)

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