FlyBase Allele Report: Dmel\Afti[NIG.8538R]

General Information

Symbol

Dmel\Afti^NIG.8538R

Species

D. melanogaster

Name

FlyBase ID

FBal0267839

Feature type

allele

Associated gene

Dmel\Afti

Associated Insertion(s)

Carried in Construct

P{NIG.8538R}

Key Links

Transgenic product class

RNAi_reagent

(Kametaka et al., 2012)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Kametaka et al., 2012)

Mutagen

in vitro construct

(Kametaka et al., 2012)

Progenitor genotype

Carried in construct

P{NIG.8538R}

Cytology

Description

UASt drives expression of a Afti RNAi construct.

(Kametaka et al., 2012)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Afti

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size, with Scer\GAL4^ey.PU

(Kametaka et al., 2012)

lethal - all die before end of pupal stage, with Scer\GAL4^Act.PU

(Kametaka et al., 2012)

some die during pupal stage, with Scer\GAL4^Act.PU

(Kametaka et al., 2012)

Phenotype Manifest In

eye, with Scer\GAL4^ey.PU

(Kametaka et al., 2012)

ommatidium, with Scer\GAL4^ey.PU

(Kametaka et al., 2012)

Detailed Description

Statement

Reference

Scer\GAL4^GMR.PU-mediated expression results in a normal eye.

Scer\GAL4^ey.PU-mediated expression results in a rough eye with irregular alignment of ommatidia and bristles. Eyes also tend to be smaller with decreased number of ommatidia. Ommatidia are of irregular sizes with excess/fewer rhabdomeres than normal; however, each ommatidium contains normally polarized photoreceptor cells, suggesting that the phenotypes are caused by defects in photoreceptor clustering during ommatidial formation.

Irregularly aligned and multiplied photoreceptor cells are observed in the eye discs of Scer\GAL4^ey.PU, Afti^NIG.3002R larvae.

The specification of R8 neurons is disturbed in Scer\GAL4^ey.PU, AP-1σ^NIG.5864R third instar larval discs.

(Kametaka et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Afti^NIG.8538R, Scer\GAL4^ey.PU is an enhancer of partially lethal - majority die phenotype of AP-1μ^NIG.9388R, Scer\GAL4^ey.PU

(Kametaka et al., 2012)

Other

Statement

Reference

AP-1μ^NIG.9388R, Afti^NIG.8538R, Scer\GAL4^ey.PU has lethal | pharate adult stage phenotype

(Kametaka et al., 2012)

Phenotype Manifest In

Other

Statement

Reference

AP-1μ^NIG.9388R, Afti^NIG.8538R, Scer\GAL4^ey.PU has head phenotype

(Kametaka et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Afti^NIG.3002R in a Scer\GAL4^ey.PU, AP-47^NIG.9388R background results in complete loss of viable adults. These animals die at the pharate stage with loss of the head.

(Kametaka et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Afti^NIG.3002R

Afti^NIG.8538R

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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