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General Information
Symbol
Dmel\RpS6dsRNA.Sym.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0268400
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences positioned at either end of the coding region drive expression of a 654bp RpS6 fragment in both sense and antisense orientation, resulting in the generation of dsRNA.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of RpS6dsRNA.Sym.Scer\UAS under the control of Scer\GAL4GMR.PU results in a smaller eye than normal which has a glassy appearance and necrotic patches.

Expression of RpS6dsRNA.Sym.Scer\UAS under the control of Scer\GAL4ey.PU does not result in an obvious mutant eye phenotype.

Expression of RpS6dsRNA.Sym.Scer\UAS under the control of Scer\GAL4amn-c651 results in a reduction in the size of the prothoracic gland in larvae compared with controls at the equivalent time point of 5 days after egg deposition (AED). In addition, these animals show an extreme developmental delay compared to wild type: at day 5 AED the larvae are smaller than control larvae, and they continue to grow and feed beyond day 10 to become giant larvae that fail to pupate. Addition of 20-hydroxyecdysone to the food results in a variable restoration of pupariation, but the final steps of metamorphosis, including the formation of adult structures, are not rescued.

Animals in which expression of RpS6dsRNA.Sym.Scer\UAS under the control of Scer\GAL4amn-c651 is delayed until the late second larval instar (using the temperature sensitive Scer\GAL80ts.αTub84B allele) are able to undergo pupation and eclose as large adults. These animals show an increase in eye size and in wing size compared to controls. If the animals are raised in the presence of 20-hydroxyecdysone, the body size of the adults is restored to normal.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement
Reference
Suppressor of
Phenotype Manifest In
Enhancer of
Statement
Reference
Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference

Expression of RpS6dsRNA.Sym.Scer\UAS under the control of Scer\GAL4GMR.PU in a CycEJP homozygous background results in an additive reduction of eye size.

Expression of RpS6dsRNA.Sym.Scer\UAS under the control of Scer\GAL4ey.PU enhances the CycEJP rough eye phenotype.

Expression of RpS6dsRNA.Sym.Scer\UAS under the control of Scer\GAL4Act.PU suppresses the CycEJP eye phenotype.

Expression of RpS6dsRNA.Sym.Scer\UAS under the control of Scer\GAL4amn-c651 from the late second larval instar (expression restricted at earlier stages using the temperature sensitive Scer\GAL80ts.αTub84B allele) partially suppresses the CycEJP small eye phenotype.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
RpS6dsRNA.Sym.Scer\UAS
RpS6dsRNA.Sym.UAS
Name Synonyms
Secondary FlyBase IDs
    References (1)