FlyBase Allele Report: Avic\GFP[RNAi.UAS.V20.1]

General Information

Symbol

Avic\GFP^{RNAi.UAS.V20.1}

Species

A. victoria

Name

FlyBase ID

FBal0268436

Feature type

allele

Associated gene

Avic\GFP

Associated Insertion(s)

Carried in Construct

P{VALIUM20-EGFP.RNAi.1}

Transgenic product class

RNAi_reagent

(Neumüller et al., 2012)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Neumüller et al., 2012)

Mutagen

in vitro construct

(Neumüller et al., 2012)

Progenitor genotype

Carried in construct

P{VALIUM20-EGFP.RNAi.1}

Cytology

Description

UAS regulatory sequences drive expression of a small hairpin RNA which is directed against EGFP (the hairpin contains an inverted repeat of CGGCATCAAGGTGAACTTCAA).

(Neumüller et al., 2012)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Avic\GFP

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

sterile | female, with MESR4^V5.miGFPi, Scer\GAL4^{VP16.nanos.UTR}

(Wissel et al., 2016)

Phenotype Manifest In

embryo, with Rab8^EGFP, Scer\GAL4^{VP16.mat.αTub67C}

(Mavor et al., 2016)

female germline cell | adult stage, with MESR4^V5.miGFPi, Scer\GAL4^{VP16.nanos.UTR}

(Wissel et al., 2016)

female germline cell | adult stage, with MESR4^V5.miGFPi/MESR4⁷⁹, Scer\GAL4^{VP16.nanos.UTR}

(Wissel et al., 2016)

female germline cell | adult stage, with Scer\GAL4^{VP16.nanos.UTR}, Tif-IA^V5.miGFPi

(Wissel et al., 2016)

female germline stem cell | adult stage, with Scer\GAL4^{VP16.nanos.UTR}, Tif-IA^V5.miGFPi

(Wissel et al., 2016)

germarium, with MESR4^V5.miGFPi, Scer\GAL4^{VP16.nanos.UTR}

(Wissel et al., 2016)

germarium, with MESR4^V5.miGFPi/MESR4⁷⁹, Scer\GAL4^{VP16.nanos.UTR}

(Wissel et al., 2016)

neuroblast | third instar larval stage, with Scer\GAL4^wor.PA, lola^{V5.miGFPi.all}

(Wissel et al., 2016)

oocyte, with MESR4^V5.miGFPi, Scer\GAL4^{VP16.nanos.UTR}

(Wissel et al., 2016)

oocyte, with MESR4^V5.miGFPi/MESR4⁷⁹, Scer\GAL4^{VP16.nanos.UTR}

(Wissel et al., 2016)

ovariole, with Scer\GAL4^{VP16.nanos.UTR}, Tif-IA^V5.miGFPi

(Wissel et al., 2016)

ovary, with MESR4^V5.miGFPi, Scer\GAL4^{VP16.nanos.UTR}

(Wissel et al., 2016)

ovary, with MESR4^V5.miGFPi/MESR4⁷⁹, Scer\GAL4^{VP16.nanos.UTR}

(Wissel et al., 2016)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^{VP16.nanos.UTR}, Tif-IA^UASp.cWa, Avic\GFP^{RNAi.UAS.V20.1} rescues Tif-IA^V5.miGFPi

(Wissel et al., 2016)

Scer\GAL4^{VP16.nanos.UTR}, Tif-IA^S610D.UASp, Avic\GFP^{RNAi.UAS.V20.1} rescues Tif-IA^V5.miGFPi

(Wissel et al., 2016)

Scer\GAL4^{VP16.nanos.UTR}, Avic\GFP^{RNAi.UAS.V20.1}, MESR4^UASp.cWa rescues MESR4^V5.miGFPi

(Wissel et al., 2016)

Partially rescues

Tif-IA^S610A.UASp, Scer\GAL4^{VP16.nanos.UTR}, Avic\GFP^{RNAi.UAS.V20.1} partially rescues Tif-IA^V5.miGFPi

(Wissel et al., 2016)

Comments

Images (0)

Mutant

Wild-type

Stocks (3)

Bloomington

66387

w^*; P{Ubi-GFP(S65T)nls}2L, P{UAS-RFP.W}2; P{VALIUM20-EGFP.RNAi.1}attP2

41556

y¹ sc^* v¹ sev²¹; P{VALIUM20-EGFP.RNAi.1}attP2

41555

y¹ sc^* v¹ sev²¹; P{VALIUM20-EGFP.RNAi.1}attP40

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Avic\GFP^{RNAi.UAS.V20.1}

Avic\GFP^{dsRNA.shRNA.Scer\UAS.1}

Avic\GFP^{dsRNA.shRNA.UAS.V20.1}

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

Open Close