FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\MESR4V5.miGFPi
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General Information
Symbol
Dmel\MESR4V5.miGFPi
Species
D. melanogaster
Name
FlyBase ID
FBal0322738
Feature type
allele
Associated gene
Dmel\MESR4
Associated Insertion(s)
TI{TI}MESR4V5.miGFPi
Carried in Construct
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
CRISPR/Cas9
(Wissel et al., 2016)
Progenitor genotype
Associated Insertion(s)
TI{TI}MESR4V5.miGFPi
Cytology
Description

A cassette comprised of the Tag:V5 epitope plus the Tag:miGFPi RNAi-effector sequence has been inserted at the N-terminal end of the MESR4 coding sequence. The presence of the Tag:miGFPi tag allows for conditional knockdown of MESR4 function using an RNAi reagent that targets EGFP.

(Wissel et al., 2016)
Allele components
Component
Use(s)
Tagged with
Tag:miGFPi
Tag:V5
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Avic\GFPdsRNA.shRNA.Scer\UAS.1 RNAi under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 in MESR4V5.miGFPi homozygotes to knock-down MESR4 by the miGFPi method (tagging a gene with a minimal GFP tag and then expressing RNAi against the tag) results in decreased size of ovaries and differentiation defects of germline cells: The ovaries contain pseudo egg chambers with polyploid nurse cells but no oocyte, the germaria are enlarged and filled with undifferentiated fusome-containing cells. Same phenotype is observed upon miGFPi-mediated knock-down of MESR4 in MESR479/MESR4V5.miGFPi transheterozygotes.

      (Wissel et al., 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      MESR4V5.miGFPi is rescued by Scer\GAL4VP16.nanos.UTR/Avic\GFPRNAi.UAS.V20.1/MESR4UASp.cWa

      (Wissel et al., 2016)
      Comments

      The decreased ovary size and germline cells differentiation defects observed in adult MESR4V5.miGFPi females upon Scer\GAL4nos.UTR.T:Hsim\VP16-driven knock-down of MESR4 using the miGFPi method (RNAi is targeted against the GFP tag inserted in a particular gene locus) is fully rescued by co-expression of MESR4Scer\UAS.P\T.cWa.

      (Wissel et al., 2016)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      MESR4V5.miGFPi
      Name Synonyms
      Secondary FlyBase IDs
        References (1)