Eogt^Δ7.4 embryos, lacking both maternal and zygotic Eogt, show a defect in the formation of the innermost layer of the apical extracellular matrix and it's attachment to the epidermis.

Eogt^Δ7.4 homozygotes and Eogt^Δ7.4/Eogt^LL00272 transheterozygotes die mostly during second-instar or second/third instar interface, but some survive until early third-instar. These mutant larvae display cuticle defects and irregular tracheal morphology. Ultrastructural analysis of the larval epidermis reveals disruption of the deposition zone of the endocuticle, leading to separation of the epidermis from the chitin layers. The larval body is not compacted and the body size is comparable to that of wild-type larvae.

Eogt^Δ7.4 embryos, lacking both maternal and zygotic Eogt exhibit largely intact tracheal networks, although the tracheal tubes can occasionally be slightly twisted or bent and dorsal trunks are occasionally broken.