FlyBase Allele Report: Dmel\Rap1[S17A.UAS]

General Information

Symbol

Dmel\Rap1^S17A.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0269539

Feature type

allele

Associated gene

Dmel\Rap1

Associated Insertion(s)

Carried in Construct

P{UAS-Rap1.S17A}

Key Links

Transgenic product class

dominant_negative_variant

(Dupuy et al., 2005)

missense_variant

(Dupuy et al., 2005)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

dominant_negative_variant

(Dupuy et al., 2005)

missense_variant

(Dupuy et al., 2005)

Mutagen

in vitro construct

(Dupuy et al., 2005)

Progenitor genotype

Carried in construct

P{UAS-Rap1.S17A}

Cytology

Description

UASt regulatory sequences drive expression of a dominant-negative form of Rap1 (carries the mutation S17A).

(Dupuy et al., 2005)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Rap1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal, with Scer\GAL4^GMR.PF

(Dupuy et al., 2005)

visible, with Scer\GAL4^GMR.PF

(Dupuy et al., 2005)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PF

(Dupuy et al., 2005)

Detailed Description

Statement

Reference

Expression of R^{S17A.Scer\UAS} under the control of Scer\GAL4^GMR.PF results in varying degrees of lethality depending on the R^{S17A.Scer\UAS} line used. Surviving adults have a strong reduced eye phenotype.

(Dupuy et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Rap1^S17A.UAS, Scer\GAL4^GMR.PF has lethal phenotype, suppressible by Epac^{Act.K549A.UAS.Tag:PM(hKRAS)}, Scer\GAL4^GMR.PF

(Dupuy et al., 2005)

Rap1^S17A.UAS, Scer\GAL4^GMR.PF has visible phenotype, suppressible by Epac^{Act.K549A.UAS.Tag:PM(hKRAS)}, Scer\GAL4^GMR.PF

(Dupuy et al., 2005)

NOT suppressed by

Statement

Reference

Rap1^S17A.UAS, Scer\GAL4^GMR.PF has lethal phenotype, non-suppressible by Ras85D^UAS.cKa, Scer\GAL4^GMR.PF

(Dupuy et al., 2005)

Rap1^S17A.UAS, Scer\GAL4^GMR.PF has lethal phenotype, non-suppressible by Rala^G20V.cMa.UAS, Scer\GAL4^GMR.PF

(Dupuy et al., 2005)

Rap1^S17A.UAS, Scer\GAL4^GMR.PF has lethal phenotype, non-suppressible by PDZ-GEF^EP388, Scer\GAL4^GMR.PF

(Dupuy et al., 2005)

Rap1^S17A.UAS, Scer\GAL4^GMR.PF has lethal phenotype, non-suppressible by C3G^UAS.Tag:MYC, Scer\GAL4^GMR.PF

(Dupuy et al., 2005)

Rap1^S17A.UAS, Scer\GAL4^GMR.PF has lethal phenotype, non-suppressible by PDZ-GEF^EP388/C3G^UAS.Tag:MYC, Scer\GAL4^GMR.PF

(Dupuy et al., 2005)

Phenotype Manifest In

Suppressed by

Statement

Reference

Rap1^S17A.UAS, Scer\GAL4^GMR.PF has eye phenotype, suppressible by Epac^{Act.K549A.UAS.Tag:PM(hKRAS)}, Scer\GAL4^GMR.PF

(Dupuy et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

The lethality caused by expression of R^{S17A.Scer\UAS} under the control of Scer\GAL4^GMR.PF is not rescued by co-expression of any one of the following: Ras85D^Scer\UAS.cKa, Rala^{G20V.cMa.Scer\UAS}, Gef26^EP388 or C3G^{Scer\UAS.T:Hsap\MYC}.

The lethality caused by expression of R^{S17A.Scer\UAS} under the control of Scer\GAL4^GMR.PF is not rescued by simultaneous co-expression of Gef26^EP388 and C3G^{Scer\UAS.T:Hsap\MYC}.

The lethality and reduced eye phenotypes caused by expression of R^{S17A.Scer\UAS} under the control of Scer\GAL4^GMR.PF can be suppressed by co-expression of Epac^{Act.K549A.Scer\UAS.T:Hsap\CAAX}.

(Dupuy et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Rap1^S17A.UAS is rescued by Scer\GAL4^GMR.PF/Rap1^V12.cHa.UAS

(Dupuy et al., 2005)

Partially rescued by

Rap1^S17A.UAS is partially rescued by Rap1^UAS.cAa/Scer\GAL4^GMR.PF

(Dupuy et al., 2005)

Comments

Co-expression of R^Scer\UAS.cAa rescues the lethality caused by expression of R^{S17A.Scer\UAS} under the control of Scer\GAL4^GMR.PF, although the rescued flies have a reduced eye phenotype.

Co-expression of R^{V12.cHa.Scer\UAS} rescues the lethality caused by expression of R^{S17A.Scer\UAS} under the control of Scer\GAL4^GMR.PF, and the rescued flies have normal sized eyes.

(Dupuy et al., 2005)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

R^{S17A.Scer\UAS}

Rap1^{S17A.Scer\UAS}

Rap1^S17A.UAS

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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