FlyBase Allele Report: Dmel\mys[RNAi.Sym.UAS]

General Information

Symbol

Dmel\mys^RNAi.Sym.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0269547

Feature type

allele

Associated gene

Dmel\mys

Associated Insertion(s)

Carried in Construct

P{UAS-mys.RNAi.Sym}

Key Links

Transgenic product class

RNAi_reagent

(Bhandari et al., 2006)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Bhandari et al., 2006)

Mutagen

in vitro construct

(Bhandari et al., 2006)

Progenitor genotype

Carried in construct

P{UAS-mys.RNAi.Sym}

Cytology

Description

UAS regulatory sequences are positioned either side of a 1.2kb fragment from the mys cDNA clone RE55238, driving both sense and antisense expression of the fragment.

(Bhandari et al., 2006)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

mys

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal smell perception, with Scer\GAL4^H24

(Bhandari et al., 2006)

abnormal smell perception, with Scer\GAL4^MT14

(Bhandari et al., 2006)

lethal, with Scer\GAL4^Act.PU

(Bhandari et al., 2006)

lethal, with Scer\GAL4^c739

(Bhandari et al., 2006)

lethal, with Scer\GAL4^dbr-72Y

(Bhandari et al., 2006)

lethal, with Scer\GAL4^Tub.PU

(Bhandari et al., 2006)

partially lethal - majority die, with Scer\GAL4^c309

(Bhandari et al., 2006)

partially lethal - majority die, with Scer\GAL4^MT14

(Bhandari et al., 2006)

viable, with Scer\GAL4^16Y

(Bhandari et al., 2006)

viable, with Scer\GAL4^59Y

(Bhandari et al., 2006)

viable, with Scer\GAL4¹⁰⁷

(Bhandari et al., 2006)

viable, with Scer\GAL4^125Y

(Bhandari et al., 2006)

viable, with Scer\GAL4^c309

(Bhandari et al., 2006)

viable, with Scer\GAL4^CG42237-C133

(Bhandari et al., 2006)

viable, with Scer\GAL4^GH146

(Bhandari et al., 2006)

viable, with Scer\GAL4^Gr5a.8.5

(Bhandari et al., 2006)

viable, with Scer\GAL4^Gr66a.3.153

(Bhandari et al., 2006)

viable, with Scer\GAL4^H24

(Bhandari et al., 2006)

visible, with Scer\GAL4^dpp.blk1

(Bhandari et al., 2006)

visible, with Scer\GAL4^en-e16E

(Bhandari et al., 2006)

Phenotype Manifest In

wing, with Scer\GAL4^dpp.blk1

(Bhandari et al., 2006)

wing, with Scer\GAL4^en-e16E

(Bhandari et al., 2006)

wing vein, with Scer\GAL4^dpp.blk1

(Bhandari et al., 2006)

wing vein, with Scer\GAL4^en-e16E

(Bhandari et al., 2006)

Detailed Description

Statement

Reference

Adults expressing mys^{dsRNA.Sym.Scer\UAS} under the control of Scer\GAL4^dpp.blk1 have a substantial wrinkled area containing vein defects in the middle of the wing. The wrinkled area also contains wing blisters.

Expression of mys^{dsRNA.Sym.Scer\UAS} under the control of Scer\GAL4^en-e16E results in wrinkling, blistering and vein defects in the wing.

Expression of mys^{dsRNA.Sym.Scer\UAS} under the control of one of Scer\GAL4¹⁰⁷, Scer\GAL4^c309, Scer\GAL4^GH146, Scer\GAL4^Gr5a.8.5, Scer\GAL4^Gr66a.3.153, Scer\GAL4^C133, Scer\GAL4^59Y, Scer\GAL4^16Y or Scer\GAL4^125Y does not significantly alter avoidance to benzaldehyde in an olfactory behaviour assay.

Expression of mys^{dsRNA.Sym.Scer\UAS} under the control of Scer\GAL4^MT14 significantly reduces avoidance of benzaldehyde in an olfactory behaviour assay.

Expression of mys^{dsRNA.Sym.Scer\UAS} under the control of Scer\GAL4^H24 significantly blunts attraction to low concentrations of benzaldehyde and significantly reduces aversion to this odorant at high concentrations. Avoidance of 4-methylcyclohexanol and attraction to ethyl acetate are also disrupted and avoidance of 2-heptanone is marginally reduced when expression is driven by Scer\GAL4^H24, while avoidance of linalool and linalool are unaffected. Negative geotaxis is normal in these flies.

(Bhandari et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressor of

Statement

Reference

mys^RNAi.Sym.UAS/Scer\GAL4^slbo.2.6 is a suppressor of border follicle cell phenotype of Scer\GAL4^slbo.2.6, mop^UAS.cCa

(Chen et al., 2012)

mys^RNAi.Sym.UAS/Scer\GAL4^slbo.2.6 is a suppressor of border follicle cell phenotype of Scer\GAL4^slbo.2.6, mop^RNAi.UAS.cCa

(Chen et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of mys^{dsRNA.Sym.Scer\UAS} suppresses the border cell cluster dissociation seen in the developing egg chamber when mop^Scer\UAS.cCa is expressed under the control of Scer\GAL4^slbo.2.6.

Expression of mys^{dsRNA.Sym.Scer\UAS} suppresses the border cell cluster dissociation seen in the developing egg chamber when mop^{dsRNA.Scer\UAS.cCa} is expressed under the control of Scer\GAL4^slbo.2.6.

(Chen et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

mys^RNAi.Sym.UAS is rescued by mys^UAS.cBa/Scer\GAL4^H24

(Bhandari et al., 2006)

Comments

Co-expression of mys^Scer\UAS.cBa rescues the behavioural defects in response to the odorant benzaldehyde which are seen in flies expressing mys^{dsRNA.Sym.Scer\UAS} under the control of Scer\GAL4^H24.

(Bhandari et al., 2006)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

mys^RNAi.Sym.UAS

mys^{dsRNA.Sym.Scer\UAS}

mys^{dsRNA.Sym.UAS}

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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