FlyBase Allele Report: Dmel\Dop1R1[UAS.Tango]

General Information

Symbol

Dmel\Dop1R1^UAS.Tango

Species

D. melanogaster

Name

FlyBase ID

FBal0269826

Feature type

allele

Associated gene

Dmel\Dop1R1

Associated Insertion(s)

Carried in Construct

P{UAS-Dop1R1-Tango}

Also Known As

DopR-Tango

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Inagaki et al., 2012)

Progenitor genotype

Carried in construct

P{UAS-Dop1R1-Tango}

Cytology

Description

UAS regulatory sequences drive expression of two coding regions separated by a 2A peptide sequence that are designed to be used to report neuromodulator release in vivo using the 'Tango' system (PMID:18165312). The upstream coding region encodes a tethered transcription factor which is composed of the Dop1R1 receptor fused via a Tag:CS(TEVp) cleavage site to a lexA::UnkAD driver (the driver is tagged with the Tag:HA epitope) (this coding region is represented in FlyBase by the Dop1R1^UAS.Tango allele). The downstream coding region consists of Arr1 fused to the TEVp protease (this coding region is represented in FlyBase by the Arr1::TEV\NIa^UAS.Tango allele). Upon activation of the Dop1R1 receptor by its ligand, the Arr1 ::TEVp protein is recruited, resulting in cleavage at the Tag:CS(TEVp) site. This allows the Tag:HA-tagged lexA::UnkAD driver to translocate to the nucleus, where it can drive expression of a reporter that is controlled by lexAop regulatory sequences.