FlyBase Allele Report: Dmel\Eph[KD]

General Information

Symbol

Dmel\Eph^KD

Species

D. melanogaster

Name

FlyBase ID

FBal0269902

Feature type

allele

Associated gene

Dmel\Eph

Associated Insertion(s)

TI{TI}Eph^KD

Carried in Construct

Key Links

Allele class

hypomorphic allele - molecular evidence

Mutagen

ends-in gene targeting

Nature of the Allele

Allele class

hypomorphic allele - molecular evidence

(Dearborn et al., 2012)

Mutagen

ends-in gene targeting

(Dearborn et al., 2012)

Progenitor genotype

Associated Insertion(s)

TI{TI}Eph^KD

Cytology

Description

Tandem partial duplication of the Eph locus. One copy is untranslatable and the other bears a 3' deletion encompassing the entire kinase domain. This is predicted to be a partial loss-of-function allele, which still retains reverse-signalling and kinase-independent activities. A w[+] marker and a single FRT site are present between the two copies of Eph.

(Dearborn et al., 2012)

Allele components

Component

Use(s)

Also carries

FRT

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal learning

(Dearborn et al., 2012)

abnormal neuroanatomy | third instar larval stage

(Dearborn et al., 2012)

female semi-sterile

(Dearborn et al., 2012)

partially lethal - majority die

(Dearborn et al., 2012)

Phenotype Manifest In

lobula | third instar larval stage

(Dearborn et al., 2012)

medulla anlage | third instar larval stage

(Dearborn et al., 2012)

Detailed Description

Statement

Reference

Homozygotes display approximately 50% developmental mortality; survivors display normal external adult morphology. Homozygous females exhibit a 60-90% rate of sterility. Olfactory-based learning is significantly impaired. Although adult eye structure and gross retinal development are normal, Eph^KD third instar larvae display variable defects in the centripetal projections of medulla cortical axons toward the developing neuropil: there are gaps in the crescent-shaped neuropil and ectopic axon bundels in the cell body layer surrounding the neuropil.

All Eph^KD mutants examined exhibit at least one gap in the medulla neuropil with the majority showing more than one such gap. Half of these animals have normal lobula architecture. Large HRP[+] cortical inclusions are rare.

(Dearborn et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Eph^KD has abnormal neuroanatomy | third instar larval stage phenotype, enhanceable by Reph¹/Reph[+]

(Dearborn et al., 2012)

Eph^KD has abnormal neuroanatomy | third instar larval stage phenotype, enhanceable by Reph^k08617A/Reph[+]

(Dearborn et al., 2012)

Phenotype Manifest In

Enhanced by

Statement

Reference

Eph^KD has lobula | third instar larval stage phenotype, enhanceable by Reph¹/Reph[+]

(Dearborn et al., 2012)

Eph^KD has lobula | third instar larval stage phenotype, enhanceable by Reph^k08617A/Reph[+]

(Dearborn et al., 2012)

Eph^KD has medulla anlage | third instar larval stage phenotype, enhanceable by Reph¹/Reph[+]

(Bettencourt da Cruz et al., 2008)

Eph^KD has medulla anlage | third instar larval stage phenotype, enhanceable by Reph^k08617A/Reph[+]

(Bettencourt da Cruz et al., 2008)

Suppressor of

Statement

Reference

Eph^KD is a suppressor of eye phenotype of Ephrin^UAS.cDa, Scer\GAL4^hs.2sev

(Dearborn et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

The rough eye phenotype of Scer\GAL4^hs.2sev, Ephrin^Scer\UAS.cDa flies is suppressed by Eph^KD.

All optic lobe phenotypic features associated with Eph^KD are exacerbated by heterozygosity for Reph¹ or Reph^k08617A.

(Dearborn et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Eph^KD

eph^KD

(Dearborn et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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