FlyBase Allele Report: Dmel\Ack[86]

General Information

Symbol

Dmel\Ack⁸⁶

Species

D. melanogaster

Name

FlyBase ID

FBal0269978

Feature type

allele

Associated gene

Dmel\Ack

Associated Insertion(s)

Carried in Construct

Also Known As

DACK⁸⁶

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Abdallah et al., 2013, Zahedi et al., 2008)

Mutagen

P-element activity

(Zahedi et al., 2008)

Progenitor genotype

P{SUPor-P}KG00869

(Zahedi et al., 2008)

Cytology

Description

2.7kb deletion extending from the beginning of the second exon of Ack (54bp after the ATG start codon).

(Abdallah et al., 2013)

Imprecise excision of the progenitor insertion, resulting in a deletion that removes part of Ack.

(Zahedi et al., 2008)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3L:4,107,857..4,110,557 [+]

Comment:

Boundaries are approximate Ack⁸⁶ is described as a 2.7 kb deletion resulting from the imprecise excision of P{SUPor-P}KG00869 that removes the first and part of the second exon of Ack and extends to 54 bases after the ATG start codon. It's not clear if the upstream boundary is at the insertion site or beyond.

(Abdallah et al., 2013)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female fertile

(Abdallah et al., 2013)

male sterile

(Abdallah et al., 2013)

viable

(Zahedi et al., 2008)

visible

(Schoenherr et al., 2012)

Phenotype Manifest In

elongation stage spermatid

(Abdallah et al., 2013)

eye

(Schoenherr et al., 2012)

individualization stage spermatid

(Schoenherr et al., 2012)

spermatozoon

(Abdallah et al., 2013)

sperm individualization complex

(Schoenherr et al., 2012)

Detailed Description

Statement

Reference

Most homozygous flies eclose with no morphological defects. Homozygous females are fertile.

Homozygous males are completely sterile and their seminal vesicles contain no sperm. The maintenance of germ stem cells and germ cell cytokinesis appears normal in the mutant testes. Properly coiled spermatids are not detected in the mutant testes. The total number of elongated cysts in the mutant testes is normal, but the number of pre-investment cone assembly cysts is reduced, and the number of cysts in which the investment cone has departed is increased compared to wild type. This suggests that the spermatids proceed to individualisation early but fail to coil properly, resulting in an accumulation of cysts in which the investment cone has departed.

(Abdallah et al., 2013)

The individualisation complexes are displaced from the outer region of the testis coil to the inner region in mutant individualising spermatids. No mature sperm are present in the seminal vesicle in mutant males.

Homozygotes have slightly smaller eyes than normal.

(Schoenherr et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Ack⁸⁶ has male sterile phenotype, suppressible by Mmus\Tnk2^βTub85D.FL

(Abdallah et al., 2013)

NOT suppressed by

Statement

Reference

Ack⁸⁶ has male sterile phenotype, non-suppressible by Ack-like^{βTub85D.FL.GFP}

(Abdallah et al., 2013)

Ack⁸⁶ has male sterile phenotype, non-suppressible by Mmus\Tnk1^βTub85D.FL

(Abdallah et al., 2013)

Enhancer of

Statement

Reference

Ack⁸⁶/Ack⁸⁶ is an enhancer of visible phenotype of hid^GMR.PG

(Schoenherr et al., 2012)

Other

Statement

Reference

Ack⁸⁶, Mmus\Tnk2^βTub85D.FL has male fertile phenotype

(Abdallah et al., 2013)

Ack[+]/Ack⁸⁶, rpr^GMR.PH has visible phenotype

(Schoenherr et al., 2012)

Ack[+]/Ack⁸⁶, rpr^GMR.PH has lethal | pharate adult stage phenotype

(Schoenherr et al., 2012)

Phenotype Manifest In

Suppressed by

Statement

Reference

Ack⁸⁶ has spermatozoon phenotype, suppressible by Mmus\Tnk2^βTub85D.FL

(Abdallah et al., 2013)

Enhancer of

Statement

Reference

Ack⁸⁶/Ack⁸⁶ is an enhancer of eye phenotype of hid^GMR.PG

(Schoenherr et al., 2012)

Other

Statement

Reference

Ack[+]/Ack⁸⁶, rpr^GMR.PH has eye phenotype

(Schoenherr et al., 2012)

Ack⁸⁶, dpp^hr27/dpp[+] has embryo | dorsal closure stage phenotype

(Zahedi et al., 2008)

Ack⁸⁶, dpp[+]/dpp^hr92 has embryo | dorsal closure stage phenotype

(Zahedi et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

Ack-like^{βTub85D.FL.T:Avic\GFP} fails to rescue the sterility of Ack⁸⁶ males.

(Abdallah et al., 2013)

Homozygosity for Ack⁸⁶ enhances the reduced eye size caused by W^GMR.PG.

Ack⁸⁶/+ flies expressing rpr^GMR.PH fail to eclose. The pharate adults have eyes that are partially ablated. The ablated eye area is replaced by a hole in the head cuticle where eye tissue would normally reside and the optic lobe region of the brain is visible. The few female adult escapers (no male escapers are seen) have an eye that is slightly reduced in size compared to flies expressing rpr^GMR.PH in a wild-type background.

(Schoenherr et al., 2012)

dpp^hr27/+ ; Ack⁸⁶/Ack⁸⁶ embryos show dorsal closure defects at a relatively low frequency, but which is significantly higher than the frequency seen in either dpp^hr27/+ or Ack⁸⁶/Ack⁸⁶ single mutant embryos.

dpp^hr92/+ ; Ack⁸⁶/Ack⁸⁶ embryos show dorsal closure defects at a relatively low frequency, but which is significantly higher than the frequency seen in either dpp^hr92/+ or Ack⁸⁶/Ack⁸⁶ single mutant embryos.

(Zahedi et al., 2008)

Xenogenetic Interactions

Statement

Reference

Mmus\Tnk2^βTub85D.FL restores fertility and sperm formation in Ack⁸⁶ males.

Mmus\Tnk1^βTub85D.FL fails to rescue the sterility of Ack⁸⁶ males.

(Abdallah et al., 2013)

Complementation and Rescue Data

Rescued by

Ack⁸⁶ is rescued by Ack^βTub85D.FL

(Abdallah et al., 2013)

Ack⁸⁶ is rescued by Ack^{βTub85D.FL.mCherry}