FlyBase Allele Report: Dmel\ImpL2[NIG.15009R]

General Information

Symbol

Dmel\ImpL2^NIG.15009R

Species

D. melanogaster

Name

FlyBase ID

FBal0272063

Feature type

allele

Associated gene

Dmel\ImpL2

Associated Insertion(s)

Carried in Construct

P{NIG.15009R}

Also Known As

UAS-ImpL2-RNAi, ImpL2 RNAi, ImpL2^RNAi, ImpL2-i

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Japanese National Institute of Genetics, 2012.5.21)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Japanese National Institute of Genetics, 2012.5.21)

Mutagen

in vitro construct

(Japanese National Institute of Genetics, 2012.5.21)

Progenitor genotype

Carried in construct

P{NIG.15009R}

Cytology

Description

UASt regulatory sequences drive expression of a 500bp inverted repeat.

(Japanese National Institute of Genetics, 2012.5.21)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-15009R

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates intestinal cancer

modeled by yki^{S111A.S168A.S250A.UAS.Tag:V5}

(Wang et al., 2023)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of ImpL2^NIG.15009R in adult intestinal stem cells (under the control of Scer\GAL4^esg-NP5130 and limited to the adult stage using Scer\GAL80^ts.αTub84B) has no effect on trehalose levels.

(Kwon et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

ImpL2^NIG.15009R, Scer\GAL4^esg.PU is a suppressor of visible | adult stage phenotype of Scer\GAL4^esg.PU, yki^{S111A.S168A.S250A.UAS.Tag:V5}

(Wang et al., 2023)

ImpL2^NIG.15009R, Scer\GAL4^NP5130 is a suppressor of visible phenotype of Scer\GAL4^NP5130, yki^{S111A.S168A.S250A.UAS.Tag:V5}

(Kwon et al., 2015)

ImpL2^NIG.15009R, Scer\GAL4^NP5130 is a suppressor of abnormal locomotor behavior | adult stage phenotype of Scer\GAL4^NP5130, yki^{S111A.S168A.S250A.UAS.Tag:V5}

(Kwon et al., 2015)

Phenotype Manifest In

Suppressor of

Statement

Reference

ImpL2^NIG.15009R, Scer\GAL4^NP5130 is a suppressor | partially of dorsal longitudinal indirect flight muscle phenotype of Scer\GAL4^NP5130, yki^{S111A.S168A.S250A.UAS.Tag:V5}

(Saavedra et al., 2023)

ImpL2^NIG.15009R, Scer\GAL4^NP5130 is a suppressor | partially of adult somatic muscle cell phenotype of Scer\GAL4^NP5130, yki^{S111A.S168A.S250A.UAS.Tag:V5}

(Saavedra et al., 2023)

ImpL2^NIG.15009R, Scer\GAL4^esg.PU is a suppressor of adult abdomen phenotype of Scer\GAL4^esg.PU, yki^{S111A.S168A.S250A.UAS.Tag:V5}

(Wang et al., 2023)

ImpL2^NIG.15009R, Scer\GAL4^esg.PU is a suppressor of adult abdominal fat cell phenotype of Scer\GAL4^esg.PU, yki^{S111A.S168A.S250A.UAS.Tag:V5}

(Wang et al., 2023)

ImpL2^NIG.15009R, Scer\GAL4^NP5130 is a suppressor of ovary phenotype of Scer\GAL4^NP5130, yki^{S111A.S168A.S250A.UAS.Tag:V5}

(Kwon et al., 2015)

ImpL2^NIG.15009R, Scer\GAL4^NP5130 is a suppressor of wing phenotype of Scer\GAL4^NP5130, yki^{S111A.S168A.S250A.UAS.Tag:V5}

(Kwon et al., 2015)

ImpL2^NIG.15009R, Scer\GAL4^NP5130 is a suppressor of mitochondrion phenotype of Scer\GAL4^NP5130, yki^{S111A.S168A.S250A.UAS.Tag:V5}

(Kwon et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of ImpL2^NIG.15009R suppresses the increase in trehalose levels seen in flies expressing yki^{S111A.S168A.S250A.Scer\UAS.T:SV5\V5} (under the control of Scer\GAL4^esg-NP5130 and limited to the adult stage using Scer\GAL80^ts.αTub84B). The overall shape of the midguts is unchanged.

The wasting of ovaries seen in flies expressing yki^{S111A.S168A.S250A.Scer\UAS.T:SV5\V5} (under the control of Scer\GAL4^esg-NP5130 and limited to the adult stage using Scer\GAL80^ts.αTub84B) is suppressed by ImpL2^NIG.15009R.

The climbing defects and downturned wings seen in flies expressing yki^{S111A.S168A.S250A.Scer\UAS.T:SV5\V5} (under the control of Scer\GAL4^esg-NP5130 and limited to the adult stage using Scer\GAL80^ts.αTub84B) are suppressed by ImpL2^NIG.15009R.

The mitochondrial defects seen in flies expressing yki^{S111A.S168A.S250A.Scer\UAS.T:SV5\V5} (under the control of Scer\GAL4^esg-NP5130 and limited to the adult stage using Scer\GAL80^ts.αTub84B) are suppressed by ImpL2^NIG.15009R. Muscle ATP levels are also reduced.

The bloating phenotype seen in flies expressing yki^{S111A.S168A.S250A.Scer\UAS.T:SV5\V5} (under the control of Scer\GAL4^esg-NP5130 and limited to the adult stage using Scer\GAL80^ts.αTub84B) is suppressed by ImpL2^NIG.15009R.

(Kwon et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

ImpL2^NIG.15009R

ImpL2^RNAi

(Figueroa-Clarevega and Bilder, 2015, Owusu-Ansah et al., 2013)

Name Synonyms

Secondary FlyBase IDs

References (12)

Report Sections

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