FlyBase Allele Report: Dmel\icln[NIG.4924R]

General Information

Symbol

Dmel\icln^NIG.4924R

Species

D. melanogaster

Name

FlyBase ID

FBal0274440

Feature type

allele

Associated gene

Dmel\icln

Associated Insertion(s)

Carried in Construct

P{NIG.4924R}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Japanese National Institute of Genetics, 2012.5.21)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Japanese National Institute of Genetics, 2012.5.21)

Mutagen

in vitro construct

(Japanese National Institute of Genetics, 2012.5.21)

Progenitor genotype

Carried in construct

P{NIG.4924R}

Cytology

Description

UASt regulatory sequences drive expression of a 500bp inverted repeat.

(Japanese National Institute of Genetics, 2012.5.21)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-4924R

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal flight, with Dcr-2^UAS.cUa, Scer\GAL4^Mef2.PR

(Borg et al., 2016)

abnormal locomotor behavior | adult stage, with Dcr-2^UAS.cUa, Scer\GAL4^Mef2.PR

(Borg et al., 2016)

lethal, with Dcr-2^UAS.cUa, Scer\GAL4^Mef2.PR

(Borg et al., 2016)

lethal, with Scer\GAL4^c179

(Borg et al., 2016)

lethal, with Scer\GAL4^da.PU

(Borg et al., 2016)

lethal, with Scer\GAL4^how-24B

(Borg et al., 2016)

partially lethal - majority die, with Dcr-2^UAS.cUa, Scer\GAL4^RapGAP1-OK6

(Borg et al., 2016)

partially lethal - majority die, with Scer\GAL4^c179

(Borg et al., 2016)

partially lethal - majority die, with Scer\GAL4^G7

(Borg et al., 2016)

partially lethal - majority die, with Scer\GAL4^how-24B

(Borg et al., 2016)

viable, with Dcr-2^UAS.cUa, Scer\GAL4^elav.PU

(Borg et al., 2016)

viable, with Dcr-2^UAS.cUa, Scer\GAL4^Mef2.PR

(Borg et al., 2016)

viable, with Dcr-2^UAS.cUa, Scer\GAL4^RapGAP1-OK6

(Borg et al., 2016)

viable, with Scer\GAL4^elav.PU

(Borg et al., 2016)

viable, with Scer\GAL4^G7

(Borg et al., 2016)

viable, with Scer\GAL4^Mef2.PR

(Borg et al., 2016)

viable, with Scer\GAL4^RapGAP1-OK6

(Borg et al., 2016)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of icln^NIG.4924R under the control of any of the following: Scer\GAL4^Mef2.PR, Scer\GAL4^RapGAP1-OK6 (both with Dcr-2^Scer\UAS.cUa), Scer\GAL4^c179, Scer\GAL4^how-24B, Scer\GAL4^G7 or Scer\GAL4^da.PU (all without Dcr-2^Scer\UAS.cUa) reduces the adult viability to a varying degree (up to a point of complete lethality in some cases) at at least one of these two temperatures: 29[o]C and 25[o]C (with more severe effects being observed at 29[o]C). Expression under Scer\GAL4^Mef2.PR, Scer\GAL4^RapGAP1-OK6 (both without Dcr-2^Scer\UAS.cUa) or Scer\GAL4^elav.PU (with or without Dcr-2^Scer\UAS.cUa) does not significantly decrease adult viability at either temperature.

Expression of icln^NIG.4924R (together with Dcr-2^Scer\UAS.cUa to enhance RNAi efficiency) under the control of Scer\GAL4^Mef2.PR results in severe flight defects with most flies being completely flightless, the adult flies also display significant drop in locomotor activity both during day and at night compared to controls.

(Borg et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

icln^NIG.4924R is rescued by icln^UAS.cBa/Scer\GAL4^Mef2.PR

(Borg et al., 2016)

Partially rescued by

icln^NIG.4924R is partially rescued by icln^UAS.cBa/Scer\GAL4^da.PU

(Borg et al., 2016)

Comments

The lethality of flies expressing icln^NIG.4924R under the control of Scer\GAL4^da.PU and the flightless phenotype observed in flies expressing icln^Scer\UAS.cBa under the Scer\GAL4^Mef2.PR driver is partially rescued and fully rescued, respectively, by co-expression of icln^Scer\UAS.cBa.

(Borg et al., 2016)

Images (0)

Mutant

Wild-type

Stocks (2)

NIG-Fly

4924R-2

P{NIG.4924R}2

4924R-3

P{NIG.4924R}3

Notes on Origin

Discoverer