FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\edRNAi.UAS.intra
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General Information
Symbol
Dmel\edRNAi.UAS.intra
Species
D. melanogaster
Name
FlyBase ID
FBal0276908
Feature type
allele
Associated gene
Dmel\ed
Associated Insertion(s)
Carried in Construct
P{UAS-ed.RNAi.intra}
Key Links
Transgenic product class
RNAi_reagent
(Chang et al., 2011)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Chang et al., 2011)
Mutagen
in vitro construct
(Chang et al., 2011)
Progenitor genotype
Carried in construct
P{UAS-ed.RNAi.intra}
Cytology
Description

UAS regulatory sequences drive expression of an inverted repeat encoding exon 7 of ed.

(Chang et al., 2011)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
ed
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Larval wing disc clones expressing eddsRNA.Scer\UAS.intra sort out from the surrounding wild type cells, exhibiting both rounded and smooth contours rather than the jagged border seen in controls. The wild type cells surrounding the clone assemble a ring of actomyosin cable at the interface and the clone cells themselves develop an apical constriction that is inversely proportional to the size of the clone.

      edΔintra.Scer\UAS.T:Zzzz\FLAG twinspot clones generated in the dorsal compartment of larval wing discs expressing eddsRNA.Scer\UAS.intra under the control of Scer\GAL4ap-md544 are not surrounded by a prominent actomyosin cable. The clone cells do sort out to some degree from the ed deficient neighbouring cells, but as in wild type the clonal border is jagged. However, the clone cells do show strong apical constriction.

      (Chang et al., 2011)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Suppressed by
      NOT suppressed by
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of fredScer\UAS.T:Zzzz\FLAG rescues the cell sorting phenotype seen in wing disc clones expressing eddsRNA.Scer\UAS.intra. The surrounding wild type cells no longer assemble an actomyosin cable and the boundary between the mutant clone and neighbouring wild type cells remains smooth.

      Expression of ed::NrgScer\UAS.T:Zzzz\FLAG is unable to suppress the cell sorting phenotypes seen in larval wing disc clones expressing eddsRNA.Scer\UAS.intra. An actomyosin cable still forms around the clone and the boundary between the mutant clone and neighbouring wild type cells remains smooth.

      (Chang et al., 2011)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      edRNAi.UAS.intra is rescued by edΔintra.UAS.Tag:FLAG

      (Chang et al., 2011)
      Comments

      Expression of edΔintra.Scer\UAS.T:Zzzz\FLAG rescues the cell sorting phenotypes seen in larval wing disc clones expressing eddsRNA.Scer\UAS.intra. The surrounding wild type cells no longer assemble an actomyosin cable and the boundary between the clone and the surrounding wild type cells is no longer smooth edged, instead appearing jagged as in wild type.

      (Chang et al., 2011)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      edRNAi.UAS.intra
      eddsRNA.Scer\UAS.intra
      eddsRNA.UAS.intra
      Name Synonyms
      Secondary FlyBase IDs
        References (2)