Activating neurons that express temperature-sensitive TrpA1^Scer\UAS.cSa under the control of Scer\GAL4^4-57, and in which the Scer\FRT cassette has been excised using Scer\FLP1^fru, has no effect on courtship and copulation behaviors compared to controls.

Males expressing TrpA1^Scer\UAS.cSa under the control of Scer\GAL4^dsx.Δ2 in the presence of Scer\FLP1^{L.8xEcol\lexAop} and Ecol\lexA^R71G01 (this limits expression to the intersection of Ecol\lexA^R71G01 and Scer\FLP1^{L.8xEcol\lexAop} expression patterns as TrpA1^Scer\UAS.cSa is generated from TrpA1^{Scer\FRT.stop.Scer\UAS.cSa} by the expression of Scer\FLP1^{L.8xEcol\lexAop}) at 30[o]C show significant courtship towards freely-moving females in a vision-based courtship assay (females separated from male targets by a thin transparent plastic barrier). This contrasts with wild-type males which barely court females under these conditions. The mutant males also court constantly rotating rubber band pieces of approximately the size of a fly in this assay.