FlyBase Allele Report: Scer\GAL4[dsx.Δ2]

ScerGAL4^dsx.Δ2 drives expression in a subset of neurons in the adult CNS, including the male-specific P1 (pMP-e) neurons in the brain. These P1 neurons overlap with the expression of fru protein.

(Pan et al., 2012)

Marker for

adult doublesex neuron

(Meissner et al., 2016)

Reflects expression of

dsx

(Zhou et al., 2014, Pan et al., 2012)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal behavior | adult stage | heat sensitive, with Ecol\lexA^GMR71G01, TrpA1^{FRT.stop.UAS.Tag:MYC}

(Hoopfer et al., 2015)

abnormal courtship behavior, with dsx^RNAi.UAS.M2

(Han et al., 2022)

abnormal courtship behavior, with dsx^TH13361.N

(Han et al., 2022)

abnormal courtship behavior | heat sensitive, with Ecol\lexA^GMR71G01, TrpA1^{FRT.stop.UAS.Tag:MYC}

(Hoopfer et al., 2015)

abnormal courtship behavior | male | heat sensitive, with TrpA1^UAS.(B).cKa

(Pan et al., 2012, Pan et al., 2011)

abnormal courtship behavior | male | heat sensitive, with TrpA1^UAS.cSa

(Pan et al., 2012)

abnormal courtship behavior | male | heat sensitive, with TrpA1^UAS.Tag:MYC

(Pan et al., 2012)

abnormal courtship behavior | male limited, with Ctet\tetX^{TNT-LC.FRT.stop.UAS}, Ecol\lexA^GMR71G01, Scer\FLP1^L.8xlexAop

(Zhou et al., 2015)

abnormal courtship behavior | male limited | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.20xUAS.FRT.Venus}, Ecol\lexA^GMR71G01, Scer\FLP1^L.8xlexAop

(Zhou et al., 2015)

abnormal mating, with dsx^RNAi.UAS.M2

(Han et al., 2022)

abnormal mating, with dsx^TH13361.N

(Han et al., 2022)

abnormal mating behavior, with Ctet\tetX^{TNT-LC.FRT.stop.UAS}, Scer\FLP1^L.8xlexAop, Scer\GAL4^GMR41A01

(Zhou et al., 2014)

abnormal mating behavior, with Ctet\tetX^{TNT-LC.FRT.stop.UAS}, Scer\FLP1^L.8xlexAop, Scer\GAL4^GMR71G01

(Zhou et al., 2014)

abnormal mating behavior | heat sensitive, with Scer\FLP1^L.8xlexAop, Scer\GAL4^GMR41A01, TrpA1^{FRT.stop.UAS.Tag:MYC}

(Zhou et al., 2014)

abnormal mating behavior | heat sensitive, with Scer\FLP1^L.8xlexAop, Scer\GAL4^GMR71G01, TrpA1^{FRT.stop.UAS.Tag:MYC}

(Zhou et al., 2014)

abnormal mating behavior | heat sensitive, with TrpA1^UAS.(B).cKa

(Zhou et al., 2014)

abnormal sex-determination | adult stage, with dsx^TH13361.N

(Han et al., 2022, Han et al., 2022)

abnormal sex-determination | adult stage, with dsx^TH13362.N

(Han et al., 2022)

abnormal sex-determination | adult stage, with dsx^{UAS.F.Tag:FLAG}

(Han et al., 2022)

abnormal sex-determination | adult stage, with dsx^{UAS.F.Tag:FLAG}, dsx^{f01649-d09625}

(Han et al., 2022)

abnormal sex-determination | adult stage, with dsx^{UAS.M2.Tag:FLAG}

(Han et al., 2022)

abnormal sex-determination | adult stage, with dsx^{UAS.M2.Tag:FLAG}, dsx^{f01649-d09625}

(Han et al., 2022)

abnormal sex-determination | adult stage, with dsx^{UAS.M.Tag:MYC}

(Han et al., 2022)

abnormal sex-determination | adult stage, with dsx^{UAS.M.Tag:MYC}, dsx^{f01649-d09625}

(Han et al., 2022)

abnormal song, with Ctet\tetX^{TNT-LC.FRT.stop.UAS}, Ecol\lexA^GMR13H01, Scer\FLP1^L.8xlexAop

(Shirangi et al., 2016)

abnormal song, with Ecol\lexA^GMR13H01, Hsap\KCNJ2^{FRT.STOP.10x.UAS.GFP}, Scer\FLP1^L.8xlexAop

(Shirangi et al., 2016)

abnormal song | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.20xUAS.FRT.Venus}, Ecol\lexA^GMR13H01, Scer\FLP1^L.8xlexAop

(Shirangi et al., 2016)

visible | adult stage, with dsx^TH13361.N

(Han et al., 2022)

visible | adult stage, with dsx^TH13362.N

(Han et al., 2022)

visible | adult stage, with dsx^{UAS.F.Tag:FLAG}

(Han et al., 2022)

visible | adult stage, with dsx^{UAS.F.Tag:FLAG}, dsx^{f01649-d09625}

(Han et al., 2022)

visible | adult stage, with dsx^{UAS.M2.Tag:FLAG}

(Han et al., 2022)

visible | adult stage, with dsx^{UAS.M2.Tag:FLAG}, dsx^{f01649-d09625}

(Han et al., 2022)

visible | adult stage, with dsx^{UAS.M.Tag:MYC}

(Han et al., 2022)

visible | adult stage, with dsx^{UAS.M.Tag:MYC}, dsx^{f01649-d09625}

(Han et al., 2022)

Phenotype Manifest In

Detailed Description

Statement

Reference

Inhibition (using Ctet\TeTxLC^FRT.stop.UAS or Hsap\KCNJ2^{FRT.STOP.10x.UAS.GFP}) of Scer\GAL4^dsx.Δ2 neurons expressing Scer\FLP1^L.8xlexAop under the control of Ecol\lexA^GMR13H01 in males results in almost no sine song during courtship, but a similar amount of pulse song to controls; the pulse song has an increase in inter-pulse interval, which is significant in the case of Ctet\TeTxLC^FRT.stop.UAS, but no difference in carrier frequency or amplitude, compared to controls.

Acute optogenetic activation (using Cnoc\ChR1^{Cs.20xUAS.FRT.Venus} with 660 nm (red) light activation) of Scer\GAL4^dsx.Δ2 neurons expressing Scer\FLP1^L.8xlexAop under the control of Ecol\lexA^GMR13H01 in males, in the absence of females, results in single wing extension and sine song similar to that of controls courting a female, but little pulse song.

(Shirangi et al., 2016)

Thermogenetic activation (using TrpA1^{FRT.stop.UAS.Tag:MYC} at 29[o]C with Scer\FLP1^L.8xlexAop) of cells expressing Scer\GAL4^dsx.Δ2 and Ecol\lexA^GMR71G01 in adult males significantly increases aggressive lunging behavior and wing extensions towards other males, compared to controls.

(Hoopfer et al., 2015)

Inhibition (using Ctet\TeTxLC^FRT.stop.UAS with Scer\FLP1^L.8xlexAop) of neurons expressing Ecol\lexA^GMR71G01 and Scer\GAL4^dsx.Δ2 significantly reduces male-male courtship chaining responses to pulse song, compared to controls.

Optogenetic activation (using Cnoc\ChR1^{Cs.20xUAS.FRT.Venus} with Scer\FLP1^L.8xlexAop and activating with 655 nm (red) light) of neurons expressing Ecol\lexA^GMR71G01 and Scer\GAL4^dsx.Δ2 significantly increases male-male courtship chaining, compared to controls.

(Zhou et al., 2015)

Thermogenetic activation (using TrpA1^UAS.(B).cKa at 27[o]C) of Scer\GAL4^dsx.Δ2 cells in adult virgin females significantly increases copulation within 10 min of introduction to a male, compared to baseline, unlike controls.

Thermogenetic activation (using TrpA1^{FRT.stop.UAS.Tag:MYC} with Scer\FLP1^L.8xlexAop at 27[o]C) of cells expressing Scer\GAL4^dsx.Δ2 and either Ecol\lexA^GMR41A01 or Ecol\lexA^GMR71G01 in mature adult virgin females, but not immature or mated females, significantly increases copulation within 10 min of introduction to a male, compared to baseline, unlike controls.

Inhibition (using Ctet\tetX^{TNT-LC.FRT.stop.UAS} with Scer\FLP1^L.8xlexAop) of cells expressing Scer\GAL4^dsx.Δ2 and either Ecol\lexA^GMR41A01 or Ecol\lexA^GMR71G01 in adult virgin females significantly reduces copulation within 10 min of introduction to a male, compared to controls.

(Zhou et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Scer\GAL4^dsx.Δ2

dsx^GAL4(Δ2)

(Zhao et al., 2024, Meissner et al., 2016, Zhou et al., 2014, Pan et al., 2012, Pan et al., 2011)

dsx^GAL4

(Zhang et al., 2018, Zhou et al., 2015, Zhou et al., 2014)

dsx^GAL4Δ2

(Hoopfer et al., 2015)

dsx^Gal4(Δ2)

(Shirangi et al., 2016)

Name Synonyms

Secondary FlyBase IDs

References (18)

Report Sections

Open Close