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General Information
Symbol
Ecol\lexAGMR71G01
Species
E. coli
Name
FlyBase ID
FBal0277015
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
R71G01-LexA
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

A 3844 base pair fragment from the flanking non-coding or intronic region of Vsx2 is fused upstream of a Drosophila core synthetic promoter (DSCP) followed by sequence encoding a lexA::p65 driver. The driver sequence has been optimized for Drosophila codon usage. An Hsp70 transcriptional terminator is present.

Allele components
Product class / Tool use(s)
Associated Sequence Features
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Information
Statement
Reference

A FLP/FRT intersectional strategy shows expression of Ecol\lexAGMR71G01 in adult doublesex pC1 (female and male) neurons when combined with Scer\GAL4dsx.Δ2.

Expression driven by Ecol\lexAGMR71G01 was restricted to a subset of adult fruitless pMP-e (male) neurons when combined with Scer\GAL4dsx.Δ2 in a FLP based intersection strategy.

Ecol\lexAGMR71G01 drives expression in a subset of adult doublesex pC1 (male) neuron that also have expression driven by Ecol\lexAfru.P1.

Ecol\lexAGMR71G01 drives expression in a subset of neurons in the adult CNS, including the male-specific P1 (pMP-e) neurons in the brain. The P1 neurons overlap with the expression of Scer\GAL4fru.P1.B or Scer\GAL4dsx-GAL4 in males. A small number of abdominal ganglion neurons and glial cells are also detected when expressing both Ecol\lexAGMR71G01 and Scer\GAL4dsx-GAL4.

 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Thermogenetic activation (using TrpA1FRT.stop.UAS.Tag:MYC at 29[o]C with Scer\FLP1L.8xlexAop) of cells expressing Scer\GAL4dsx.Δ2 and Ecol\lexAGMR71G01 in adult males significantly increases aggressive lunging behavior and wing extensions towards other males, compared to controls.

Inhibition (using Ctet\TeTxLCFRT.stop.UAS with Scer\FLP1L.8xlexAop) of neurons expressing Ecol\lexAGMR71G01 and Scer\GAL4dsx.Δ2 significantly reduces male-male courtship chaining responses to pulse song, compared to controls.

Optogenetic activation (using Cnoc\ChR1Cs.20xUAS.FRT.Venus with Scer\FLP1L.8xlexAop and activating with 655 nm (red) light) of neurons expressing Ecol\lexAGMR71G01 and Scer\GAL4dsx.Δ2 significantly increases male-male courtship chaining, compared to controls.

Inhibition (using Ctet\tetXTNT-LC.FRT.stop.UAS with Scer\FLP1L.8xlexAop) of cells expressing both Scer\GAL4dsx.Δ2 and Ecol\lexAGMR71G01 in adult virgin females significantly reduces copulation within 10 min of introduction to a male, compared to controls.

Thermogenetic activation (using TrpA1FRT.stop.UAS.Tag:MYC with Scer\FLP1L.8xlexAop at 27[o]C) of cells expressing both Scer\GAL4dsx.Δ2 and Ecol\lexAGMR71G01 in mature adult virgin females, but not immature or mated females, significantly increases copulation within 10 min of introduction to a male, compared to baseline, unlike controls.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Ecol\lexAGMR71G01
Ecol\lexAR71G01
Name Synonyms
Secondary FlyBase IDs
    References (14)