FB2026_02 , released June 18, 2026
Allele: Dmel\TrhnRNAi.Sym.UAS
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General Information
Symbol
Dmel\TrhnRNAi.Sym.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0277070
Feature type
allele
Associated gene
Dmel\Trhn
Associated Insertion(s)
Carried in Construct
P{UAS-Trhn.RNAi.Sym}
Key Links
Transgenic product class
RNAi_reagent
(Neckameyer, 2010)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Neckameyer, 2010)
Mutagen
in vitro construct
(Neckameyer, 2010)
Progenitor genotype
Carried in construct
P{UAS-Trhn.RNAi.Sym}
Cytology
Description

UAS regulatory sequences positioned at either end of the coding region drive expression of a full-length Trh cDNA, in both sense and antisense orientation, resulting in the generation of dsRNA.

(Neckameyer, 2010)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Trhn
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Larvae expressing TrhdsRNA.Sym.Scer\UAS under the control of Scer\GAL4elav-C155 exhibit a reduced feeding rate compared to controls. There is no change in pupal size or time to pupariation, but there is a correlation between the reduced feeding rate and increased pupal survival.

      (Neckameyer and Bhatt, 2012)

      Animals with reduced Trh through expression of TrhdsRNA.Sym.Scer\UAS under the control of Scer\GAL4elav.Switch.PO (included by 6mg/ml RU486) exhibit a feeding rate indistinguishable from controls, when fed with 5-HTP. Changes in 5-HT levels either by feeding 5-HTP or by reducing Trh levels has no effect on locomotion.

      Decreased levels of Trh through expression of TrhdsRNA.Sym.Scer\UAS under the control of Scer\GAL4elav-C155 directly correlate with reduced feeding relative to controls. General motor activity is unaffected. Feeding 5-HTP to these flies results in a highly increased appetite, while locomotor control is unaffected.

      There is a correlative increase in neuronal arborization with decreasing levels of neuronal 5-HT in animals expressing TrhdsRNA.Sym.Scer\UAS under the control of Scer\GAL4elav-C155. In general, the number of total varicosities increase with TrhdsRNA.Sym.Scer\UAS expression.

      Analysis of the architecture of serotonergic fibers projecting to the proventriculus in Scer\GAL4elav.Switch.PO/TrhdsRNA.Sym.Scer\UAS animals, inducing neuronal expression of the transgene during the second instar stage, reveals no differences in arborization of fibers, varicosity number, number of large varicosities, or in varicosity area.

      (Neckameyer, 2010)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of pledsRNA.Sym.Scer\UAS and TrhdsRNA.Sym.Scer\UAS under the control of Scer\GAL4elav-C155, does not affect larval feeding, unlike expression of either single transgene. When the same combination is placed under the control of either Scer\GAL4ple.PF or Scer\GAL4l.Trh.PK, feeding is reduced, consistent with knockdown of only DA or 5-HT synthesis, but not both. Gut fiber architecture is rescued to control levels by the presence of both pledsRNA.Sym.Scer\UAS and TrhdsRNA.Sym.Scer\UAS.

      (Neckameyer and Bhatt, 2012)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      TrhdsRNA.Sym.Scer\UAS
      TrhdsRNA.Sym.UAS
      TrhnRNAi.Sym.UAS
      TrhndsRNA.Sym.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (3)