FlyBase Allele Report: Scer\GAL4[l.Trhn.PK]

FB2026_01 , released March 12, 2026

FB2026_01 , released March 12, 2026

Allele: Scer\GAL4^l.Trhn.PK

General Information

Symbol

Scer\GAL4^l.Trhn.PK

Species

S. cerevisiae

Name

FlyBase ID

FBal0246203

Feature type

allele

Associated gene

Associated Insertion(s)

Carried in Construct

P{Trhn-GAL4.long}

Also Known As

Trh-GAL4, Trhn-Gal4

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Alekseyenko et al., 2010)

Progenitor genotype

Carried in construct

P{Trhn-GAL4.long}

Cytology

Description

A 1.7kb fragment of Trh regulatory sequences is cloned upstream of sequence encoding a GAL4 driver.

(Alekseyenko et al., 2010)

Allele components

Component

Use(s)

Regulatory region(s)

Encoded product / tool

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

serotonergic neuron

(Burgos et al., 2018)

adult serotonergic LP2 neuron

(Alekseyenko et al., 2010)

FMRFamide SE2 neuron

(Alekseyenko et al., 2010)

adult protocerebrum | restricted

(Alekseyenko et al., 2010)

posterior lateral protocerebrum | restricted

(Alekseyenko et al., 2010)

adult serotonergic SEM neuron

(Xu et al., 2016, Alekseyenko et al., 2010)

adult serotonergic SE1 neuron

(Xu et al., 2016, Alekseyenko et al., 2010)

adult brain | restricted

(Xu et al., 2016)

adult serotonergic PMPD neuron

(Xu et al., 2016)

adult serotonergic PMPM neuron | subset

(Xu et al., 2016)

adult serotonergic IP neuron | subset

(Xu et al., 2016)

adult serotonergic PLP neuron

(Xu et al., 2016)

adult serotonergic LP2 neuron | subset

(Xu et al., 2016)

adult serotonergic LP3 neuron | subset

(Xu et al., 2016)

adult serotonergic SE2 neuron

(Xu et al., 2016)

adult serotonergic ALP neuron | subset

(Xu et al., 2016)

adult serotonergic T1 neuron | subset

(Xu et al., 2016)

adult serotonergic T2 neuron | subset

(Xu et al., 2016)

adult serotonergic T3 neuron | subset

(Xu et al., 2016)

serotonergic neuron | subset of adult abdomen

(Xu et al., 2016)

adult serotonergic ADMP neuron

(Xu et al., 2016)

serotonergic IP1 neuron 1

(Xu et al., 2016)

Additional Information

Statement

Reference

Drives expression in the posterior medial protocerebrum (PMP), the AMP (anterior medial protocerebrum) and the ALP (anterior lateral protocerebrum). Drives expression in several identified neurons, including the adult serotonergic LP2, SE1, and SE3 neurons, and the FMRFamide SE2 neuron.

(FlyBase Curators, 2017)

ScerGAL4^l.Trh.PK drives expression in all SP1 neurons, 3-4 SP2 neurons, 4-6 IP neurons, all LP1 neurons, 4-9 LP2 neurons, 0-1 and in three clusters of serotonergic neurons in the protocerebrum, one dorsal to LP1, another in the dorsal part and one the anterior part, where it drives expression in 0-1 adult serotonergic LP3 neuron, 1-3 adult serotonergic AP neurons and all adult serotonergic DP neuron. It also drives expression in small numbers (1-5) of non-serotonergic neurons adjacent to SP1, SP2, IP, LP1, LP2, LP3, T2 and T3.

(Xu et al., 2016)

ScerGAL4^l.Trh.PK is seen to be expressed in the posterior medial protocerebrum (PMP), the AMP (anterior medial protocerebrum) and the ALP (anterior lateral protocerebrum).

(Alekseyenko et al., 2010)

Marker for

serotonergic neuron

(Burgos et al., 2018)

Reflects expression of

(Burgos et al., 2018, Alekseyenko et al., 2010)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult ventral nerve cord, with sbb^GD10734

(Yilmazer et al., 2016)

serotonergic neuron, with sbb^GD10734

(Yilmazer et al., 2016)

Detailed Description

Statement

Reference

Inactivation of a subset of Scer\GAL4^l.Trh.PK cells (using Ctet\tetX^{TNT-LC.FRT.stop.UAS} and Scer\FLP1³³⁵ with Scer\GAL80^tsh-GAL80) in males slightly, but significantly reduces copulation duration, with no effect on the frequency of successful matings or copulation latency, compared to controls.

(Jois et al., 2018)

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Trhn^RNAi.Sym.UAS/Scer\GAL4^l.Trhn.PK is a suppressor of abnormal feeding behavior | larval stage phenotype of Scer\GAL4^ple.PF, ple^RNAi.Sym.UAS

(Neckameyer and Bhatt, 2012)

Phenotype Manifest In

Suppressor of

Statement

Reference

Trhn^RNAi.Sym.UAS/Scer\GAL4^l.Trhn.PK is a suppressor of serotonergic neuron phenotype of Scer\GAL4^ple.PF, ple^RNAi.Sym.UAS

(Neckameyer and Bhatt, 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

Pngl^UASp.cFa/Scer\GAL4^l.Trhn.PK partially rescues Pngl^PL

(Hope et al., 2022)

Fails to rescue

Pngl^C303A.UASp/Scer\GAL4^l.Trhn.PK fails to rescue Pngl^PL

(Hope et al., 2022)

Scer\GAL4^l.Trhn.PK/Ns3^UAS.Venus fails to rescue Ns3^G0431

(Hartl et al., 2013)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

38388

w¹¹¹⁸; P{Trhn-GAL4.long}2

38389

w¹¹¹⁸; P{Trhn-GAL4.long}3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Scer\GAL4^l.Trh.PK

Scer\GAL4^l.Trhn.PK

Name Synonyms

Secondary FlyBase IDs

References (58)

Report Sections