FB2026_02 , released June 18, 2026
Allele: Dmel\Top3α54
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General Information
Symbol
Dmel\Top3α54
Species
D. melanogaster
Name
FlyBase ID
FBal0277291
Feature type
allele
Associated gene
Dmel\Top3α
Associated Insertion(s)
P{?EP}Top3α54
Carried in Construct
Key Links
Genomic Maps

Allele class

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - molecular evidence

(Wu et al., 2010)
Mutagen
P-element activity
(Wu et al., 2010)
Progenitor genotype
P{EP}Top3αEP2272
(Wu et al., 2010)
Associated Insertion(s)
P{?EP}Top3α54
Cytology
Description

Imprecise excision of the progenitor insertion, resulting in a 2437bp deletion of part of the Top3α coding sequence, starting from 203bp downstream of the first translational initiation codon. 331bp of the original insertion remain.

(Wu et al., 2010)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
delins
2L:19,498,441..19,500,877 [+]
Comment:

2437 bp deletion, resulting from the imprecise excision of P{EP}Top3alphaEP2272, starting from 203bp downstream of the first translational initiation codon. 331bp of the original insertion remain.

(Wu et al., 2010)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Homozygotes develop through the third larval instar stage with a 9 day delay compared to heterozygous siblings, and die at pupation. The brains of the homozygous third instar larvae are normal in size, but they completely lack imaginal discs. Salivary gland and fat body nuclei are comparable in size to wild type.

      (Wu et al., 2010)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      Top3α54 is rescued by Top3αEYFP

      (Wu et al., 2010)

      Top3α54 is rescued by Top3αM26L.EYFP

      (Wu et al., 2010)
      Partially rescued by

      Top3α54 is partially rescued by Top3αΔCT2.EYFP

      (Chen et al., 2012)

      Top3α54 is partially rescued by Top3αM1L.EYFP

      (Wu et al., 2010)
      Not rescued by

      Top3α54 is not rescued by Top3αNT.EYFP

      (Chen et al., 2012)
      Comments

      Top3αNT.T:Avic\GFP-EYFP fails to rescue the lethality of Top3α54 homozygotes.

      One copy of Top3αΔCT2.T:Avic\GFP-EYFP rescues the lethality of approximately 5% of Top3α54 homozygotes. Two copies of Top3αΔCT2.T:Avic\GFP-EYFP rescue the lethality of approximately 10% of Top3α54 homozygotes.

      (Chen et al., 2012)

      Top3αM26L.T:Avic\GFP-EYFP fully rescues the viability defects of Top3α54 animals and the rescued animals are fertile.

      Top3αM1L.T:Avic\GFP-EYFP fully rescues the viability defects of Top3α54 animals. However, the rescued flies show fertility defects in both sexes.

      (Wu et al., 2010)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Top3α54
      top3α54
      (Chen et al., 2012, Wu et al., 2010)
      Name Synonyms
      Secondary FlyBase IDs
        References (2)