FlyBase Allele Report: Dmel\gish[D187N.UAS.Tag:MYC]

General Information

Symbol

Dmel\gish^{D187N.UAS.Tag:MYC}

Species

D. melanogaster

Name

FlyBase ID

FBal0277526

Feature type

allele

Associated gene

Dmel\gish

Associated Insertion(s)

Carried in Construct

P{UAS-gish.KD.myc}

Also Known As

UAS-myc-gish^KD

Key Links

Transgenic product class

missense_variant

(Gault et al., 2012)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Gault et al., 2012)

Mutagen

in vitro construct

(Gault et al., 2012)

Progenitor genotype

Carried in construct

P{UAS-gish.KD.myc}

Cytology

Description

UASt regulatory sequences drive expression of a kinase dead D187N mutant of gish that is tagged with Tag:MYC.

(Gault et al., 2012)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

gish

Tagged with

Tag:MYC

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Suppressor of

Statement

Reference

gish^{D187N.UAS.Tag:MYC}/Scer\GAL4^Bx-MS1096 is a non-suppressor of visible phenotype of Scer\GAL4^Bx-MS1096, smo^PKA12.UAS

(Li et al., 2016)

gish^{D187N.UAS.Tag:MYC}/Scer\GAL4^Bx-MS1096 is a non-suppressor of visible phenotype of Scer\GAL4^Bx-MS1096, gish[+]/gish^Δ4, smo^PKA12.UAS

(Li et al., 2016)

Phenotype Manifest In

NOT Suppressor of

Statement

Reference

gish^{D187N.UAS.Tag:MYC}/Scer\GAL4^Bx-MS1096 is a non-suppressor of wing phenotype of Scer\GAL4^Bx-MS1096, smo^PKA12.UAS

(Li et al., 2016)

gish^{D187N.UAS.Tag:MYC}/Scer\GAL4^Bx-MS1096 is a non-suppressor of wing phenotype of Scer\GAL4^Bx-MS1096, gish[+]/gish^Δ4, smo^PKA12.UAS

(Li et al., 2016)

Scer\GAL4^en-e16E/gish^{D187N.UAS.Tag:MYC} is a non-suppressor of wing hair | increased number phenotype of Scer\GAL4^en-e16E, mwh^GD8165

(Gault et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of gish^{D187N.Scer\UAS.T:Hsap\MYC} fails to rescue the fused wing phenotype of flies expressing smo^{PKA12.Scer\UAS} under the control of Scer\GAL4^Bx-MS1096.

The enhancement of the fused wing phenotype of flies expressing smo^{PKA12.Scer\UAS} under the control of Scer\GAL4^Bx-MS1096 by gish^Δ4/+ is not reversed by co-expression of gish^{D187N.Scer\UAS.T:Hsap\MYC}.

(Li et al., 2016)

Co-expression of gish^{D187N.Scer\UAS.T:Hsap\MYC} with mwh^GD8165 via Scer\GAL4^en-e16E fails to suppress the mwh^GD8165-induced wing hair phenotype.

(Gault et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Co-expression of gish^{D187N.Scer\UAS.T:Hsap\MYC} with gish^GD10588 under the control of Scer\GAL4^en-e16E fails to rescue the trichome phenotype.

(Gault et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

41766

w^*; P{UAS-gish.KD.myc}B187-7

41767

P{UAS-gish.KD.myc}B187-12, w^*

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

gish^{D187N.Scer\UAS.T:Hsap\MYC}

gish^{D187N.UAS.Tag:MYC}

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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