FlyBase Allele Report: Dmel\DCTN1-p150[UAS.Tag:HA]

General Information

Symbol

Dmel\DCTN1-p150^UAS.Tag:HA

Species

D. melanogaster

Name

FlyBase ID

FBal0277612

Feature type

allele

Associated gene

Dmel\DCTN1-p150

Associated Insertion(s)

Carried in Construct

P{UAS-DCTN1-p150.HA}

Key Links

Transgenic product class

wild_type

(Lloyd et al., 2012)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Lloyd et al., 2012)

Mutagen

in vitro construct

(Lloyd et al., 2012)

Progenitor genotype

Carried in construct

P{UAS-DCTN1-p150.HA}

Cytology

Description

UAS regulatory sequences drive expression of wild-type DCTN1-p150 which is tagged with a Tag:HA epitope.

(Lloyd et al., 2012)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

DCTN1-p150

Tagged with

Tag:HA

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | adult stage | progressive, with Scer\GAL4^ple.PF

(Hosaka et al., 2017)

abnormal neurophysiology | third instar larval stage, with Scer\GAL4^RapGAP1-OK6

(Hosaka et al., 2017)

abnormal size | third instar larval stage, with Scer\GAL4^Toll-6-D42

(Hosaka et al., 2017)

short lived, with Scer\GAL4^ple.PF

(Hosaka et al., 2017)

Phenotype Manifest In

neuronal dense core vesicle | third instar larval stage, with Scer\GAL4^Toll-6-D42

(Hosaka et al., 2017)

terminal bouton | third instar larval stage, with Scer\GAL4^Toll-6-D42

(Hosaka et al., 2017)

Detailed Description

Statement

Reference

Adults expressing DCTN1-p150^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^ple.PF exhibit a significant decrease in lifespan and a significant, progressive decrease in climbing performance (detected at day 45, but not day 5, post eclosion)but 45 days old adults do not display a significant decrease in spontaneous locomotor activity or significant changes in the number of PPM1/2, PPM3 or PPL1 neurons, as compared to controls.

The third instar larval neuromuscular junctions of individuals expressing DCTN1-p150^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^Toll-6-D42 display a significant increase in the density of dense core vesicles in distal, but not proximal, terminal boutons, without significantly affecting their size, as compared to controls; the respective axonal dense core vesicles exhibit a significant increase in size, as compared to controls.

Neurotransmission across the third instar larval neuromuscular junction of individuals expressing DCTN1-p150^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^RapGAP1-OK6 presents a significant increase in the frequency of miniature excitatory junction potential, but does not display significant defects in miniature excitatory junction potential amplitude, paired-pulse ratio, excitatory junction potential amplitude or quantal content, as compared to controls.

(Hosaka et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

DCTN1-p150^UAS.Tag:HA, Scer\GAL4^ple.PF has abnormal locomotor behavior | adult stage | progressive phenotype, suppressible by TBPH[+]/TBPH^Δ23

(Hosaka et al., 2017)

DCTN1-p150^UAS.Tag:HA, Scer\GAL4^ple.PF has short lived phenotype, suppressible by TBPH[+]/TBPH^Δ23

(Hosaka et al., 2017)

DCTN1-p150^UAS.Tag:HA, Scer\GAL4^Toll-6-D42 has abnormal size | third instar larval stage phenotype, suppressible by TBPH[+]/TBPH^Δ23

(Hosaka et al., 2017)

Suppressor of

Statement

Reference

Scer\GAL4^ple.PF/DCTN1-p150^UAS.Tag:HA is a suppressor of abnormal locomotor behavior | dominant | adult stage | progressive phenotype of TBPH^Δ23

(Hosaka et al., 2017)

Scer\GAL4^ple.PF/DCTN1-p150^UAS.Tag:HA is a suppressor of short lived | dominant phenotype of TBPH^Δ23

(Hosaka et al., 2017)

NOT Suppressor of

Statement

Reference

Scer\GAL4^ple.PF/DCTN1-p150^UAS.Tag:HA is a non-suppressor of abnormal locomotor behavior | dominant | adult stage phenotype of TBPH^Δ23

(Hosaka et al., 2017)

Phenotype Manifest In

Enhanced by

Statement

Reference

DCTN1-p150^UAS.Tag:HA, Scer\GAL4^Toll-6-D42 has neuronal dense core vesicle | third instar larval stage phenotype, enhanceable by TBPH[+]/TBPH^Δ23

(Hosaka et al., 2017)

DCTN1-p150^UAS.Tag:HA, Scer\GAL4^Toll-6-D42 has terminal bouton | third instar larval stage phenotype, enhanceable by TBPH[+]/TBPH^Δ23

(Hosaka et al., 2017)

NOT Enhanced by

Statement

Reference

DCTN1-p150^UAS.Tag:HA, Scer\GAL4^Toll-6-D42 has neuronal dense core vesicle | third instar larval stage phenotype, non-enhanceable by TBPH[+]/TBPH^Δ23

(Hosaka et al., 2017)

DCTN1-p150^UAS.Tag:HA, Scer\GAL4^Toll-6-D42 has terminal bouton | third instar larval stage phenotype, non-enhanceable by TBPH[+]/TBPH^Δ23

(Hosaka et al., 2017)

Suppressed by

Statement

Reference

DCTN1-p150^UAS.Tag:HA, Scer\GAL4^Toll-6-D42 has neuronal dense core vesicle | third instar larval stage phenotype, suppressible by TBPH[+]/TBPH^Δ23

(Hosaka et al., 2017)

Enhancer of

Statement

Reference

DCTN1-p150^UAS.Tag:HA/Scer\GAL4^Toll-6-D42 is an enhancer of terminal bouton | third instar larval stage phenotype of TBPH^Δ23

(Hosaka et al., 2017)

DCTN1-p150^UAS.Tag:HA/Scer\GAL4^Toll-6-D42 is an enhancer of neuronal dense core vesicle | third instar larval stage phenotype of TBPH^Δ23

(Hosaka et al., 2017)

Suppressor of

Statement

Reference

DCTN1-p150^UAS.Tag:HA/Scer\GAL4^Toll-6-D42 is a suppressor of neuronal dense core vesicle | third instar larval stage phenotype of TBPH^Δ23

(Hosaka et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The decreases in adult climbing performance and in lifespan exhibited upon the expression of DCTN1-p150^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^ple.PF or exhibited by TBPH^Δ23 heterozygotes are suppressed in individuals that simultaneously express DCTN1-p150^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^ple.PF and are heterozygous for TBPH^Δ23. The expression of DCTN1-p150^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^ple.PF also partially suppresses the decreased number of PPL1 neurons in the adult brain, but not the spontaneous locomotion defects, presented by 45 days old TBPH^Δ23 heterozygotes.

The increased size of axonal dense core vesicles and the increased density of dense core vesicles in distal terminal boutons at the third instar larval neuromuscular junction of individuals expressing DCTN1-p150^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^Toll-6-D42 are suppressed and enhanced, respectively, by TBPH^Δ23 heterozygosity.

The expression of DCTN1-p150^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^Toll-6-D42 enhances and suppresses the increased density of dense core vesicles at the distal and proximal terminal boutons, respectively, of TBPH^Δ23 heterozygous third instar larval neuromuscular junctions.

(Hosaka et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

DCTN1-p150^{Scer\UAS.T:Ivir\HA1}

DCTN1-p150^UAS.Tag:HA

Gl^{Scer\UAS.T:Ivir\HA1}

p150^WT-HA

(Lloyd et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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