FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\stmAS358A.EGFP
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General Information
Symbol
Dmel\stmAS358A.EGFP
Species
D. melanogaster
Name
FlyBase ID
FBal0277668
Feature type
allele
Associated gene
Dmel\stmA
Associated Insertion(s)
Carried in Construct
P{stmA-EGFP.S358A}
Key Links
Transgenic product class
missense_variant
(Vijayakrishnan et al., 2010)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Vijayakrishnan et al., 2010)
Mutagen
in vitro construct
(Vijayakrishnan et al., 2010)
Progenitor genotype
Carried in construct
P{stmA-EGFP.S358A}
Cytology
Description

Amino acid replacement: S358A.

(Vijayakrishnan et al., 2010)

Nucleotide substitution: A?G.

(Vijayakrishnan et al., 2010)

A genomic fragment of stmA with the native promoter and a mutation within the lipase domain is tagged with a 3'-terminal EGFP sequence and cloned into the pUAST plasmid.

(Vijayakrishnan et al., 2010)

Nucleotide substitution: G?C.

(Vijayakrishnan et al., 2010)
Allele components
Component
Use(s)
Regulatory region(s)
stmA
Encoded product / tool
stmA
Tagged with
EGFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      stmArev499/stmA1 mutants carrying stmAS358A.T:Avic\GFP-EGFP are viable.

      At permissive temperature (25[o]C) stmA1 flies expressing stmAS358A.T:Avic\GFP-EGFP in the Garland cells take TR-vidin tracer into discrete puncta/vesicles to the same level as wild-type controls. At the 37[o]C restrictive temperature, while wild-type cells continue to display robust tracer uptake, no tracer-positive vesicles are found. This indicates that there is a complete block in Garland cell endocytosis in stmA1 flies carrying stmAS358A.T:Avic\GFP-EGFP.

      (Vijayakrishnan et al., 2010)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      stmAS358A.EGFP rescues stmA1/stmArev499

      (Vijayakrishnan et al., 2010)
      Fails to rescue

      stmAS358A.EGFP fails to rescue stmA1

      (Vijayakrishnan et al., 2010)

      stmAS358A.EGFP fails to rescue stmArev499

      (Vijayakrishnan et al., 2010)
      Comments

      stmAS358A.T:Avic\GFP-EGFP-expressing stmA1/stmArev499 animals behave as wild-type, with no paralysis, compared to stmA1/stmArev499 mutants, that experience paralysis within 5 minutes of exposure to the restrictive temperature.

      (Vijayakrishnan et al., 2010)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      rboS358A
      (Zhang et al., 2017)
      stmAS358A.EGFP
      stmAS358A.T:Avic\GFP-EGFP
      Name Synonyms
      Secondary FlyBase IDs
        References (2)