FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Nep4UAS.A
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General Information
Symbol
Dmel\Nep4UAS.A
Species
D. melanogaster
Name
FlyBase ID
FBal0277820
Feature type
allele
Associated gene
Dmel\Nep4
Associated Insertion(s)
Carried in Construct
P{UAS-Nep4.A}
Also Known As
UAS-Nep4A
Key Links
Transgenic product class
wild_type
(Panz et al., 2012)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Panz et al., 2012)
Mutagen
in vitro construct
(Panz et al., 2012)
Progenitor genotype
Carried in construct
P{UAS-Nep4.A}
Cytology
Description

UASt regulatory sequences drive expression of the full-length Nep4 A isoform.

(Panz et al., 2012)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Nep4
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  heart conduction disease
      CEA
      (Schiemann et al., 2022)
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of high levels of Nep4Scer\UAS.A under the control of Scer\GAL4Mef2.5xCD results in a reduced crawling speed in second instar larvae compared to wild type. This reduction appears to be caused by a combination of fewer and weaker muscle contractions than normal.

      The body wall muscles of third instar larvae expressing Nep4Scer\UAS.A under the control of Scer\GAL4Mef2.5xCD show degeneration and a loss of sarcomeric structure. Actin-myosin distribution is disorganised, the I-band pattern is completely disrupted and Z-bands are staggered. Nuclei with dispersed chromatin, swollen and severely fragmented mitochondria and swollen sarcoplasmic reticuli are seen.

      Larvae expressing Nep4Scer\UAS.A under the control of Scer\GAL4how-24B show muscle degeneration.

      (Panz et al., 2012)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Nep4Scer\UAS.A
      Nep4UAS.A
      Name Synonyms
      Secondary FlyBase IDs
        References (3)