FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\diaFH1FH2.UASp.EGFP
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General Information
Symbol
Dmel\diaFH1FH2.UASp.EGFP
Species
D. melanogaster
Name
FlyBase ID
FBal0277863
Feature type
allele
Associated gene
Dmel\dia
Associated Insertion(s)
Carried in Construct
P{UASp-dia.FH1FH2.EGFP}
Key Links
Transgenic product class
inframe_deletion
(Homem and Peifer, 2009)
Mutagen
Nature of the Allele
Transgenic product class
inframe_deletion
(Homem and Peifer, 2009)
Mutagen
in vitro construct
(Homem and Peifer, 2009)
Progenitor genotype
Carried in construct
P{UASp-dia.FH1FH2.EGFP}
Cytology
Description

UASp regulatory sequences drive expression of the FH1 and FH2 domains of dia. The protein is tagged at the N-terminal end with EGFP (the tag is joined to the dia coding region via a HRYTSLYKKAGSAAAPFT linker).

(Homem and Peifer, 2009)
Allele components
Component
Use(s)
Regulatory region(s)
UASp
Encoded product / tool
dia
Tagged with
EGFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of diaFH1FH2.Scer\UAS.P\T.T:Avic\GFP-EGFP under the control of Scer\GAL4en-e16E results in the dynamic lamellipodia and filopodia of the leading edge cells being replaced by lamellipodia with ruffled edges and by fan-shaped lamellipodia with radial actin arrays not seen in wild-type lamellipodia. These lamellipodia are larger than in wild type. Filopodial number, length and lifetime are all significantly decreased compared to wild type. Many of the remaining "filopodia" arise from fan-shaped lamellipodia and do not grow substantially after their appearance, in contrast to wild-type filopodia.

      (Homem and Peifer, 2009)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of enaScer\UAS.T:Avic\GFP and diaFH1FH2.Scer\UAS.P\T.T:Avic\GFP-EGFP under the control of Scer\GAL4en-e16E in embryos results in fewer filopodia but larger lamellipodia at the leading edge.

      (Homem and Peifer, 2009)
      Xenogenetic Interactions
      Statement
      Reference

      Co-expression of Zzzz\actAFP4mito.Scer\UAS.T:Avic\GFP-EGFP largely restores filopodia number and length to wild type in leading edge cells expressing diaFH1FH2.Scer\UAS.P\T.T:Avic\GFP-EGFP under the control of Scer\GAL4en-e16E. Fewer ruffle-like lamellipodia are seen and those that form are nearer wild type in size.

      (Homem and Peifer, 2009)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      diaFH1FH2.Scer\UAS.P\T.T:Avic\GFP-EGFP
      diaFH1FH2.UASp.EGFP
      Name Synonyms
      Secondary FlyBase IDs
        References (3)