FlyBase Allele Report: Dmel\mir-982[UAS.DsRed(Unk)]

General Information

Symbol

Dmel\mir-982^{UAS.DsRed(Unk)}

Species

D. melanogaster

Name

FlyBase ID

FBal0278114

Feature type

allele

Associated gene

Dmel\mir-982

Associated Insertion(s)

Carried in Construct

P{UAS-DsRed-mir-982.L}

Key Links

Transgenic product class

wild_type

(Bejarano et al., 2012)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Bejarano et al., 2012)

Mutagen

in vitro construct

(Bejarano et al., 2012)

Progenitor genotype

Carried in construct

P{UAS-DsRed-mir-982.L}

Cytology

Description

UAS regulatory sequences drive expression of a DsRed(Unk) fluorescent protein. A genomic fragment containing the mir-982 pre-miRNA hairpin has been inserted into the DsRed(Unk) 3'UTR.

(Bejarano et al., 2012)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

mir-982

Tagged with

DsRed(Unk)

red fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal, with Scer\GAL4^da.PU

(Mohammed et al., 2014, Bejarano et al., 2012)

lethal, with Scer\GAL4^elav.PU

(Mohammed et al., 2014)

lethal, with Scer\GAL4^ey.PU

(Mohammed et al., 2014)

visible, with Scer\GAL4^Bx-MS1096

(Bejarano et al., 2012, Bejarano et al., 2012)

visible, with Scer\GAL4^sd-SG29.1

(Bejarano et al., 2012, Bejarano et al., 2012)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PU

(Mohammed et al., 2014)

macrochaeta, with Scer\GAL4^Bx-MS1096

(Mohammed et al., 2014)

macrochaeta, with Scer\GAL4^ptc.PU

(Mohammed et al., 2014)

macrochaeta, with Scer\GAL4^sca.PU

(Mohammed et al., 2014)

microchaeta, with Scer\GAL4^Eq

(Mohammed et al., 2014)

wing, with Scer\GAL4^Bx-MS1096

(Mohammed et al., 2014, Bejarano et al., 2012)

wing, with Scer\GAL4^da.PU

(Mohammed et al., 2014)

wing, with Scer\GAL4^sd.PU

(Mohammed et al., 2014)

wing, with Scer\GAL4^sd-SG29.1

(Bejarano et al., 2012, Bejarano et al., 2012)

wing margin, with Scer\GAL4^Bx-MS1096

(Mohammed et al., 2014)

wing margin, with Scer\GAL4^sd.PU

(Mohammed et al., 2014)

wing margin, with Scer\GAL4^sd-SG29.1

(Bejarano et al., 2012)

wing vein, with Scer\GAL4^Bx-MS1096

(Bejarano et al., 2012)

Detailed Description

Statement

Reference

Expression via Scer\GAL4^Bx-MS1096 results in thin notum macrochaetes and small wing with margin defects.

Expression via Scer\GAL4^GMR.PU results in rough and small eyes.

Expression via Scer\GAL4^elav.PU results in lethality (for one transgenic line).

Expression via Scer\GAL4^ptc.PU results in missing, thin and short macrochaetes.

Expression via Scer\GAL4^da.PU results in lethality and curled wings.

Expression via Scer\GAL4^Eq results in misorganized microchaetes.

Expression via Scer\GAL4^sca.PU results in missing macrochaetes.

Expression via Scer\GAL4^sd.PU results in lethality and wing with a blister or no margin.

(Mohammed et al., 2014)

Expression of mir-982^{Scer\UAS.T:Disc\RFP} under the control of Scer\GAL4^Bx-MS1096 results in smaller wings than normal.

Expression of mir-982^{Scer\UAS.T:Disc\RFP} under the control of Scer\GAL4^sd-SG29.1 results in a loss of wing margin and wing notching.

Expression of mir-982^{Scer\UAS.T:Disc\RFP} under the control of Scer\GAL4^ptc-559.1 does not result in a mutant phenotype.

(Bejarano et al., 2012)

Expression of mir-982^{Scer\UAS.T:Disc\RFP} under the control of Scer\GAL4^Bx-MS1096 results in wing vein thickening.

Expression of mir-982^{Scer\UAS.T:Disc\RFP} under the control of Scer\GAL4^sd-SG29.1 results in wing notching.

(Bejarano et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

mir-982^{Scer\UAS.T:Disc\RFP}

mir-982^{UAS.DsRed(Unk)}

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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