FlyBase Allele Report: Dmel\Khc[63]

General Information

Symbol

Dmel\Khc⁶³

Species

D. melanogaster

Name

FlyBase ID

FBal0278208

Feature type

allele

Associated gene

Dmel\Khc

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Djagaeva et al., 2012)

Progenitor genotype

Cytology

Description

Amino acid replacement: G249E.

(Djagaeva et al., 2012)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:16,269,517..16,269,517 [-]

Nucleotide change:

G16269517A

Reported nucleotide change:

C12157022T

Amino acid change:

G249E | Khc-PA

Reported amino acid change:

G249E

Comment:

Reported base location of mutation is relative to noncoding strand.

(Djagaeva et al., 2012)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cytokinesis (with Khc²⁷)

(Gallaud et al., 2014)

abnormal mitotic cell cycle (with Khc²⁷)

(Gallaud et al., 2014)

abnormal mitotic cell cycle | larval stage (with Khc²⁷)

(Métivier et al., 2019)

lethal - all die before end of pupal stage (with Df(2R)Jp6)

(Djagaeva et al., 2012)

some die during pupal stage (with Df(2R)Jp6)

(Djagaeva et al., 2012)

Phenotype Manifest In

larval brain (with Khc²⁷)

(Gallaud et al., 2014)

larval brain | larval stage (with Khc²⁷)

(Métivier et al., 2019)

Detailed Description

Statement

Reference

Khc²⁷/Khc⁶³ larval brain neuroblasts exhibit centrosome separation defects compared to controls.

(Métivier et al., 2019)

Khc²⁷/Khc⁶³ mutant brains exhibit centrosome separation defects, that lead to a small angle between centrosomes at nuclear envelope breakdown but that have no effect on spindle assembly.

(Gallaud et al., 2014)

Khc⁶³/Khc²⁷ larvae show reduced flux of neurosecretory dense core vesicles along the axons compared to wild type.

(Djagaeva et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Khc²⁷/Khc⁶³ has abnormal mitotic cell cycle | larval stage phenotype, suppressible by ens^UAS.Venus/Scer\GAL4^69B

(Métivier et al., 2019)

Phenotype Manifest In

Suppressed by

Statement

Reference

Khc²⁷/Khc⁶³ has larval brain | larval stage phenotype, suppressible by ens^UAS.Venus/Scer\GAL4^69B

(Métivier et al., 2019)

NOT Suppressor of

Statement

Reference

Khc²⁷/Khc⁶³ is a non-suppressor of mitotic spindle | third instar larval stage phenotype of Scer\GAL4^69B, ens^UAS.Venus

(Métivier et al., 2019)

Khc²⁷/Khc⁶³ is a non-suppressor of larval brain | third instar larval stage phenotype of Scer\GAL4^69B, ens^UAS.Venus

(Métivier et al., 2019)

Khc²⁷/Khc⁶³ is a non-suppressor of larval neuroblast | third instar larval stage phenotype of Scer\GAL4^69B, ens^UAS.Venus

(Métivier et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Khc⁶³

(Métivier et al., 2019, Gallaud et al., 2014, Djagaeva et al., 2012)

khc⁶³

(Hannaford et al., 2022)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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